PMID- 25515203
OWN - NLM
STAT- MEDLINE
DCOM- 20151228
LR  - 20220331
IS  - 1616-5195 (Electronic)
IS  - 1616-5187 (Linking)
VI  - 15
IP  - 4
DP  - 2015 Apr
TI  - PEG-Poly(L-alanine) thermogel as a 3D scaffold of bone-marrow-derived mesenchymal 
      stem cells.
PG  - 464-72
LID - 10.1002/mabi.201400426 [doi]
AB  - Bone-marrow-derived mesenchymal stem cells (BMSCs) were cultured in 
      three-dimensional (3D) scaffolds formed by temperature-sensitive sol-to-gel 
      transition of BMSC-suspended poly(ethylene glycol)-poly(L-alanine) (PEG-PA) 
      aqueous solutions. A commercialized thermogelling 3D scaffold of Matrigel was 
      used for the comparative study. The cells maintained their spherical shapes in 
      the PEG-PA thermogel, whereas fibrous cell morphologies were observed in the 
      Matrigel. Type II collagen and myogenic differentiation factor 1 were dominantly 
      expressed in the PEG-PA thermogel. On the other hand, a significant extent of 
      type III beta-tubulin was expressed in the Matrigel in addition to type II collagen 
      and myogenic differentiation factor 1. After confirming the dominant chondrogenic 
      differentiation of the BMSCs in the PEG-PA thermogel in in vitro study, in vivo 
      study was performed for injectable tissue engineering application of the 
      BMSCs/PEG-PA system. The cell-growing implant was formed in situ by subcutaneous 
      injection of the BMSC-suspended PEG-PA aqueous solution to mice. In vivo study 
      also proved the excellent expressions of chondrogenic biomarkers including 
      collagen type II and sulfated glycosaminoglycan in the mouse model. This paper 
      suggests that the PEG-PA thermogel is a very promising as a 3D culture matrix as 
      well as an injectable tissue-engineering system for preferential chondrogenic 
      differentiation of the BMSCs.
CI  - (c) 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Park, Min Hee
AU  - Park MH
AD  - Department of Chemistry and Nano Science, Ewha Global Top 5 Research Program, 
      Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul, 120-750, Korea.
FAU - Moon, Hyo Jung
AU  - Moon HJ
FAU - Park, Jin Hye
AU  - Park JH
FAU - Shinde, Usha Pramod
AU  - Shinde UP
FAU - Ko, Du Young
AU  - Ko du Y
FAU - Jeong, Byeongmoon
AU  - Jeong B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141216
PL  - Germany
TA  - Macromol Biosci
JT  - Macromolecular bioscience
JID - 101135941
RN  - 0 (Drug Combinations)
RN  - 0 (Laminin)
RN  - 0 (Peptides)
RN  - 0 (Proteoglycans)
RN  - 119978-18-6 (matrigel)
RN  - 25191-17-7 (polyalanine)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - *Cell Differentiation
MH  - Cell Proliferation
MH  - Chondrogenesis
MH  - Collagen/chemistry
MH  - Drug Combinations
MH  - *In Vitro Techniques
MH  - Laminin/chemistry
MH  - Mesenchymal Stem Cells/*cytology
MH  - Mice
MH  - Peptides/chemistry
MH  - Polyethylene Glycols/chemistry
MH  - Proteoglycans/chemistry
MH  - *Tissue Engineering
MH  - Tissue Scaffolds
OTO - NOTNLM
OT  - 3D culture
OT  - BMSC
OT  - hydrogel
OT  - sol-gel transition
EDAT- 2014/12/18 06:00
MHDA- 2015/12/29 06:00
CRDT- 2014/12/18 06:00
PHST- 2014/09/23 00:00 [received]
PHST- 2014/10/28 00:00 [revised]
PHST- 2014/12/18 06:00 [entrez]
PHST- 2014/12/18 06:00 [pubmed]
PHST- 2015/12/29 06:00 [medline]
AID - 10.1002/mabi.201400426 [doi]
PST - ppublish
SO  - Macromol Biosci. 2015 Apr;15(4):464-72. doi: 10.1002/mabi.201400426. Epub 2014 
      Dec 16.