PMID- 25515798
OWN - NLM
STAT- MEDLINE
DCOM- 20151125
LR  - 20150319
IS  - 1573-6776 (Electronic)
IS  - 0141-5492 (Linking)
VI  - 37
IP  - 4
DP  - 2015 Apr
TI  - Improvement on the thermal stability and activity of plant cytosolic ascorbate 
      peroxidase 1 by tailing hyper-acidic fusion partners.
PG  - 891-8
LID - 10.1007/s10529-014-1754-4 [doi]
AB  - Cytosolic ascorbate peroxidase 1 (APX1) plays a crucial role in regulating the 
      level of plant cellular reactive oxygen species and its thermolability is 
      proposed to cause plant heat-susceptibility. Herein, several hyper-acidic fusion 
      partners, such as the C-terminal peptide tails, were evaluated for their effects 
      on the thermal stability and activity of APX1 from Jatropha curcas and 
      Arabidopsis. The hyper-acidic fusion partners efficiently improved the 
      thermostability and prevented thermal inactivation of APX1 in both plant species 
      with an elevated heat tolerance of at least 2  degrees C. These hyper-acidified 
      thermostable APX1 fusion variants are of considerable biotechnological potential 
      and can provide a new route to enhance the heat tolerance of plant species 
      especially of inherent thermo-sensitivity.
FAU - Zhang, Mengru
AU  - Zhang M
AD  - Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan 
      Province, Engineering Research Center of Sustainable Development and Utilization 
      of Biomass Energy, Ministry of Education, School of Life Sciences, Yunnan Normal 
      University, Kunming, 650500, Yunnan, China, zrcxfx@sina.com.
FAU - Gong, Ming
AU  - Gong M
FAU - Yang, Yumei
AU  - Yang Y
FAU - Li, Xujuan
AU  - Li X
FAU - Wang, Haibo
AU  - Wang H
FAU - Zou, Zhurong
AU  - Zou Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141217
PL  - Netherlands
TA  - Biotechnol Lett
JT  - Biotechnology letters
JID - 8008051
RN  - 0 (Plant Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 1.11.1.11 (Ascorbate Peroxidases)
SB  - IM
MH  - Arabidopsis/*enzymology/radiation effects
MH  - Ascorbate Peroxidases/*chemistry/genetics/*metabolism
MH  - Enzyme Stability
MH  - Jatropha/*enzymology/radiation effects
MH  - Plant Proteins/chemistry/genetics/metabolism
MH  - Recombinant Fusion Proteins/*chemistry/genetics/*metabolism
MH  - Temperature
EDAT- 2014/12/18 06:00
MHDA- 2015/12/15 06:00
CRDT- 2014/12/18 06:00
PHST- 2014/11/07 00:00 [received]
PHST- 2014/12/11 00:00 [accepted]
PHST- 2014/12/18 06:00 [entrez]
PHST- 2014/12/18 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - 10.1007/s10529-014-1754-4 [doi]
PST - ppublish
SO  - Biotechnol Lett. 2015 Apr;37(4):891-8. doi: 10.1007/s10529-014-1754-4. Epub 2014 
      Dec 17.