PMID- 25515846
OWN - NLM
STAT- MEDLINE
DCOM- 20151204
LR  - 20150311
IS  - 2192-2659 (Electronic)
IS  - 2192-2640 (Linking)
VI  - 4
IP  - 4
DP  - 2015 Mar 11
TI  - Biomineral coating increases bone formation by ex vivo BMP-7 gene therapy in 
      rapid prototyped poly(L-lactic acid) (PLLA) and poly(epsilon-caprolactone) (PCL) porous 
      scaffolds.
PG  - 621-32
LID - 10.1002/adhm.201400424 [doi]
AB  - Porousbiodegradable polymer scaffolds are widely utilized for bone tissue 
      engineering, but are not osteoconductive like calcium phosphate scaffolds. We 
      combine indirect solid freeform fabrication (SFF), ex vivo gene therapy, with 
      biomineral coating to compare the effect of biomineral coating on bone 
      regeneration for Poly (L-lactic acid) (PLLA) and Poly (epsilon-caprolactone) (PCL) 
      scaffolds with the same porous architecture. Scanning electron microscope (SEM) 
      and micro-computed tomography (mu-CT) demonstrate PLLA and PCL scaffolds have the 
      same porous architecture and are completely coated. All scaffolds are seeded with 
      human gingival fibroblasts (HGF) transduced with adenovirus encoded with either 
      bone morphogenetic protein 7 (BMP-7) or green fluorescent protein (GFP), and 
      implanted into mice subcutaneously for 3 and 10 weeks. Only scaffolds with BMP-7 
      transduced HGFs show mineralized tissue formation. At 3 weeks some blood 
      vessel-like structures are observed in coated PLLA and PCL scaffolds, but there 
      is no significant difference in bone ingrowth between the coated and uncoated 
      scaffolds for either PLLA or PCL. At 10 weeks, however, coated scaffolds (both 
      PLLA and PCL) have significantly more bone ingrowth than uncoated scaffolds, 
      which have more fibrous tissue. Coated PLLA scaffolds have improved mechanical 
      properties compared with uncoated PLLA scaffolds due to increased bone ingrowth.
CI  - (c) 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Saito, Eiji
AU  - Saito E
AD  - Department of Biomedical Engineering, 1101 Beal Ave. University of Michigan, Ann 
      Arbor, MI, 48109-2099, USA.
FAU - Suarez-Gonzalez, Darilis
AU  - Suarez-Gonzalez D
FAU - Murphy, William L
AU  - Murphy WL
FAU - Hollister, Scott J
AU  - Hollister SJ
LA  - eng
GR  - R01 AR 053379/AR/NIAMS NIH HHS/United States
GR  - R21 DE 022439/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20141216
PL  - Germany
TA  - Adv Healthc Mater
JT  - Advanced healthcare materials
JID - 101581613
RN  - 0 (Bone Morphogenetic Protein 7)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Minerals)
RN  - 0 (Polyesters)
RN  - 0 (poly(lactic acid-co-epsilon-caprolactone))
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Adenoviridae/metabolism
MH  - Animals
MH  - Bone Morphogenetic Protein 7/*genetics/*therapeutic use
MH  - Coated Materials, Biocompatible/*pharmacology
MH  - Elastic Modulus
MH  - Female
MH  - *Genetic Therapy
MH  - Green Fluorescent Proteins/metabolism
MH  - Humans
MH  - Materials Testing
MH  - Mice
MH  - Minerals/*pharmacology
MH  - Osteogenesis/*drug effects
MH  - Polyesters/*chemistry
MH  - Porosity
MH  - Spectrometry, X-Ray Emission
MH  - Tissue Scaffolds/*chemistry
MH  - X-Ray Diffraction
MH  - X-Ray Microtomography
OTO - NOTNLM
OT  - biomineralization
OT  - medical applications
OT  - polymeric materials
OT  - surface modification
OT  - tissue engineering
EDAT- 2014/12/18 06:00
MHDA- 2015/12/15 06:00
CRDT- 2014/12/18 06:00
PHST- 2014/07/21 00:00 [received]
PHST- 2014/12/18 06:00 [entrez]
PHST- 2014/12/18 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - 10.1002/adhm.201400424 [doi]
PST - ppublish
SO  - Adv Healthc Mater. 2015 Mar 11;4(4):621-32. doi: 10.1002/adhm.201400424. Epub 
      2014 Dec 16.