PMID- 25516264
OWN - NLM
STAT- MEDLINE
DCOM- 20150916
LR  - 20240102
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 11
IP  - 4
DP  - 2015 Apr
TI  - Sann-Joong-Kuey-Jian-Tang decreases the protein expression of mammalian target of 
      rapamycin but increases microtubule associated protein II light chain 3 
      expression to inhibit human BxPC‑3 pancreatic carcinoma cells.
PG  - 3160-6
LID - 10.3892/mmr.2014.3090 [doi]
AB  - Sann‑Joong‑Kuey‑Jian‑Tang (SJKJT), a Traditional Chinese Medicinal prescription, 
      has been used for the treatment of lymphadenopathy and solid tumors, and has 
      shown therapeutic potential in a number of human malignant tumor cell lines, such 
      as Hep‑G2 hepatocellular carcinoma cells. Previous mechanistic studies 
      demonstrated that SJKJT inhibited the proliferation of BxPC‑3 pancreatic 
      carcinoma cells through the extrinsic and intrinsic apoptotic pathways in vitro. 
      SJKJT was also shown to be cytotoxic to colo 205 colon cancer cells by inducing 
      autophagy in vitro. The present study therefore investigated molecular mechanisms 
      of autophagy in human BxPC‑3 pancreatic cancer cells treated with SJKJT. The 
      cytotoxic effects of SJKJT on BxPC‑3 human pancreatic carcinoma cells were 
      evaluated using an MTT assay. Furthermore, the expression of autophagy‑associated 
      proteins, including mammalian target of rapamycin (mTOR), beclin‑1, 
      autophagocytosis‑associated protein (Atg)3, Atg7, Atg5‑Atg12 and 
      microtubule‑associated protein II light chain 3 (LC3‑II), was assessed using 
      western blot analysis. The results demonstrated that BxPC‑3 cells treated with 
      SJKJT exhibited decreased expression levels of mTOR and increased expression of 
      LC3‑II protein. In addition, the expression of the beclin‑1, Atg3, Atg7 and 
      Atg5‑Atg12 proteins was increased during the first 24 h, but decreased from 48 to 
      72 h. The results showed that SJKJT inhibited the proliferation of human BxPC‑3 
      pancreatic cancer cells in vitro. A possible underlying molecular mechanism may 
      be the induction of autophagy. Further investigation into the therapeutic 
      potential of SJKJT in human pancreatic cancer is required.
FAU - Su, Chin-Cheng
AU  - Su CC
AD  - Tumor Research Center of Integrative Medicine, Changhua Christian Hospital, 
      Changhua, Taiwan 50006, R.O.C.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141215
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (BECN1 protein, human)
RN  - 0 (Beclin-1)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (MAP1LC3A protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (sann-joong-kuey-jian-tang)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Antineoplastic Agents/*pharmacology/toxicity
MH  - Apoptosis Regulatory Proteins/genetics/metabolism
MH  - Beclin-1
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Drugs, Chinese Herbal/*pharmacology/toxicity
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/genetics/metabolism
MH  - Membrane Proteins/genetics/metabolism
MH  - Microtubule-Associated Proteins/genetics/*metabolism
MH  - Pancreatic Neoplasms/drug therapy/*metabolism
MH  - TOR Serine-Threonine Kinases/genetics/*metabolism
EDAT- 2014/12/18 06:00
MHDA- 2015/09/17 06:00
CRDT- 2014/12/18 06:00
PHST- 2014/02/18 00:00 [received]
PHST- 2014/11/19 00:00 [accepted]
PHST- 2014/12/18 06:00 [entrez]
PHST- 2014/12/18 06:00 [pubmed]
PHST- 2015/09/17 06:00 [medline]
AID - 10.3892/mmr.2014.3090 [doi]
PST - ppublish
SO  - Mol Med Rep. 2015 Apr;11(4):3160-6. doi: 10.3892/mmr.2014.3090. Epub 2014 Dec 15.