PMID- 25518708
OWN - NLM
STAT- MEDLINE
DCOM- 20160203
LR  - 20150520
IS  - 1607-842X (Electronic)
IS  - 0891-6934 (Linking)
VI  - 48
IP  - 4
DP  - 2015 Jun
TI  - B cells produce less IL-10, IL-6 and TNF-alpha in myasthenia gravis.
PG  - 201-7
LID - 10.3109/08916934.2014.992517 [doi]
AB  - B cells from myasthenia gravis (MG) patients with autoantibodies (Aab) against 
      acetylcholine receptor (AChR), muscle-specific kinase (MuSK) or with no 
      detectable Aab were investigated as cytokine producing cells in this study. B 
      cells were evaluated for memory phenotypes and expressions of IL-10, IL-6 and 
      IL-12A. Induced productions of IL-10, IL-6, IL-12p40, TNF-alpha and LT from isolated 
      B cells in vitro were measured by immunoassays. MG patients receiving 
      immunosuppressive treatment had higher proportions of memory B cells compared 
      with healthy controls and untreated patients. With CD40 stimulation MG patients 
      produced significantly lower levels of IL-10, IL-6. With CD40 and B cell receptor 
      stimulation of B cells, TNF-alpha production also decreased in addition to these 
      cytokines. The lower levels of these cytokine productions were not related to 
      treatment. Our results confirm a disturbance of B cell subpopulations in MG 
      subgroups on immunosuppressive treatment. B cell derived IL-10, IL-6 and TNF-alpha 
      are down-regulated in MG, irrespective of different antibody productions. 
      Ineffective cytokine production by B cells may be a susceptibility factor in 
      dysregulation of autoimmune Aab production.
FAU - Yilmaz, Vuslat
AU  - Yilmaz V
AD  - Department of Physiology , and.
FAU - Oflazer, Piraye
AU  - Oflazer P
FAU - Aysal, Fikret
AU  - Aysal F
FAU - Parman, Yesim G
AU  - Parman YG
FAU - Direskeneli, Haner
AU  - Direskeneli H
FAU - Deymeer, Feza
AU  - Deymeer F
FAU - Saruhan-Direskeneli, Guher
AU  - Saruhan-Direskeneli G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141218
PL  - England
TA  - Autoimmunity
JT  - Autoimmunity
JID - 8900070
RN  - 0 (Autoantibodies)
RN  - 0 (Cytokines)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Interleukin-6)
RN  - 0 (Receptors, Cholinergic)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Autoantibodies/immunology
MH  - B-Lymphocytes/*immunology/*metabolism
MH  - Child
MH  - Cytokines/biosynthesis/genetics
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Immunophenotyping
MH  - Immunosuppressive Agents/therapeutic use
MH  - Interleukin-10/*biosynthesis/genetics
MH  - Interleukin-6/*biosynthesis/genetics
MH  - Lymphocyte Count
MH  - Male
MH  - Middle Aged
MH  - Myasthenia Gravis/diagnosis/genetics/*immunology/*metabolism/therapy
MH  - Phenotype
MH  - Receptors, Cholinergic/immunology
MH  - Tumor Necrosis Factor-alpha/*biosynthesis/genetics
MH  - Young Adult
OTO - NOTNLM
OT  - Autoantibody
OT  - B cell
OT  - cytokine
OT  - myasthenia gravis
EDAT- 2014/12/19 06:00
MHDA- 2016/02/04 06:00
CRDT- 2014/12/19 06:00
PHST- 2014/12/19 06:00 [entrez]
PHST- 2014/12/19 06:00 [pubmed]
PHST- 2016/02/04 06:00 [medline]
AID - 10.3109/08916934.2014.992517 [doi]
PST - ppublish
SO  - Autoimmunity. 2015 Jun;48(4):201-7. doi: 10.3109/08916934.2014.992517. Epub 2014 
      Dec 18.