PMID- 25518993
OWN - NLM
STAT- MEDLINE
DCOM- 20150917
LR  - 20161125
IS  - 1091-7691 (Electronic)
IS  - 0895-8378 (Linking)
VI  - 27
IP  - 1
DP  - 2015 Jan
TI  - Roles of oxidative damage and mitochondria-mediated apoptosis in 
      ethylbenzene-induced hepatotoxic effects in rat.
PG  - 64-73
LID - 10.3109/08958378.2014.986314 [doi]
AB  - The mechanisms underlying hepatoxic effects of ethylbenzene still remain unknown. 
      We investigated the toxic effects of ethylbenzene on liver and explored the 
      mechanism of mitochondria-mediated apoptosis pathway. Forty male Sprague-Dawley 
      rats were used as an in vivo model with ethylbenzene inhalation of 0, 
      433.5 mg/m(3), 4335 mg/m(3) and 6500 mg/m(3) for 13 weeks. Levels of 
      malondialdehyde, glutathione, glutathione peroxidase and superoxide dismutase 
      were assayed. Meanwhile, the ultrastructure of hepatic tissues was observed and 
      cell apoptosis was detected via terminal deoxynucleotidyl transferase-mediated 
      dUTP nick end labeling (TUNEL) assay. Furthermore, we investigated the expression 
      levels of mRNA and protein of bax, bcl-2, cytochrome c, caspase-9 and caspase-3 
      in rat liver tissues. Compared with control group, the malondialdehyde levels 
      were significantly elevated while glutathione levels and activities of 
      glutathione peroxidase and superoxide dismutase were decreased, respectively. The 
      mitochondria of liver appeared swollen with vacuolar structure and loss of 
      cristae in 6500 mg/m(3) ethylbenzene-treated group, and ethylbenzene induced a 
      significant increase in the percentage of apoptotic cells as compared to the 
      control group. In addition, enhanced mRNA and protein expression levels of all 
      measured genes were observed in ethylbenzene-treated groups except the decreased 
      bcl-2 expression levels. Our results indicated that ethylbenzene may induce 
      oxidative damage and apoptosis in rat liver. Mitochondrial-mediated pathway was 
      involved in the apoptosis process.
FAU - Zhang, Ming
AU  - Zhang M
AD  - Tianjin Centers for Disease Control and Prevention , Tianjin , P. R. China.
FAU - Wang, Yanrang
AU  - Wang Y
FAU - Yang, Deyi
AU  - Yang D
FAU - Zhang, Jingshu
AU  - Zhang J
FAU - Gu, Qing
AU  - Gu Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141218
PL  - England
TA  - Inhal Toxicol
JT  - Inhalation toxicology
JID - 8910739
RN  - 0 (Bax protein, rat)
RN  - 0 (Benzene Derivatives)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (RNA, Messenger)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.6.1.1 (Aspartate Aminotransferases)
RN  - EC 2.6.1.2 (Alanine Transaminase)
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Casp9 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspase 9)
RN  - GAN16C9B8O (Glutathione)
RN  - L5I45M5G0O (ethylbenzene)
SB  - IM
MH  - Alanine Transaminase/blood
MH  - Animals
MH  - Apoptosis/*drug effects/physiology
MH  - Aspartate Aminotransferases/blood
MH  - Benzene Derivatives/*toxicity
MH  - Caspase 3/genetics/metabolism
MH  - Caspase 9/genetics/metabolism
MH  - Chemical and Drug Induced Liver Injury/*etiology/genetics/metabolism/pathology
MH  - Glutathione/metabolism
MH  - Glutathione Peroxidase/metabolism
MH  - Liver/drug effects/metabolism/pathology/ultrastructure
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Mitochondria/*metabolism/pathology
MH  - Proto-Oncogene Proteins c-bcl-2/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats, Sprague-Dawley
MH  - Superoxide Dismutase/metabolism
MH  - bcl-2-Associated X Protein/genetics/metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - ethylbenzene
OT  - hepatotoxicity
OT  - mitochondria
OT  - oxidative damage
OT  - rat
EDAT- 2014/12/19 06:00
MHDA- 2015/09/18 06:00
CRDT- 2014/12/19 06:00
PHST- 2014/12/19 06:00 [entrez]
PHST- 2014/12/19 06:00 [pubmed]
PHST- 2015/09/18 06:00 [medline]
AID - 10.3109/08958378.2014.986314 [doi]
PST - ppublish
SO  - Inhal Toxicol. 2015 Jan;27(1):64-73. doi: 10.3109/08958378.2014.986314. Epub 2014 
      Dec 18.