PMID- 25520250
OWN - NLM
STAT- MEDLINE
DCOM- 20160322
LR  - 20181113
IS  - 1476-5497 (Electronic)
IS  - 0307-0565 (Print)
IS  - 0307-0565 (Linking)
VI  - 39
IP  - 5
DP  - 2015 May
TI  - Epigenetic patterns in successful weight loss maintainers: a pilot study.
PG  - 865-868
LID - 10.1038/ijo.2014.213 [doi]
AB  - DNA methylation changes occur in animal models of calorie restriction, simulating 
      human dieting, and in human subjects undergoing behavioral weight loss 
      interventions. This suggests that obese (OB) individuals may possess unique 
      epigenetic patterns that may vary with weight loss. Here, we examine whether 
      methylation patterns in leukocytes differ in individuals who lost sufficient 
      weight to go from OB to normal weight (NW; successful weight loss maintainers; 
      SWLMs) vs currently OB or NW individuals. This study examined peripheral blood 
      mononuclear cell (PBMC) methylation patterns in NW (n=16, current/lifetime BMI 
      18.5-24.9) and OB individuals (n=16, current body mass index (BMI)⩾30), and SWLM 
      (n=16, current BMI 18.5-24.9, lifetime maximum BMI ⩾30, average weight loss 57.4 
      lbs) using an Illumina Infinium HumanMethylation450 BeadArray. No leukocyte 
      population-adjusted epigenome-wide analyses were significant; however, 
      potentially differentially methylated loci across the groups were observed in 
      ryanodine receptor-1 (RYR1; P=1.54E-6), myelin protein zero-like 3 (MPZL3; 
      P=4.70E-6) and alpha 3c tubulin (TUBA3C; P=4.78E-6). In 32 obesity-related 
      candidate genes, differential methylation patterns were found in brain-derived 
      neurotrophic factor (BDNF; gene-wide P=0.00018). In RYR1, TUBA3C and BDNF, SWLM 
      differed from OB but not NW. In this preliminary investigation, leukocyte SWLM 
      DNA methylation patterns more closely resembled NW than OB individuals in three 
      gene regions. These results suggest that PBMC methylation is associated with 
      weight status.
FAU - Huang, Yen-Tsung
AU  - Huang YT
AD  - Department of Epidemiology, School of Public Health, Brown University, 
      Providence, Rhode Island, USA.
FAU - Maccani, Jennifer Z J
AU  - Maccani JZJ
AD  - Tobacco Center of Regulatory Science, Department of Public Health Sciences, 
      Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA.
FAU - Hawley, Nicola L
AU  - Hawley NL
AD  - Department of Psychiatry and Human Behavior, Alpert Medical School of Brown 
      University, Providence, Rhode Island, USA.
AD  - The Miriam Hospital Weight Control and Diabetes Research Center, Providence, 
      Rhode Island, USA.
AD  - Department of Chronic Disease Epidemiology, Yale School of Public Health, New 
      Haven, Connecticut, USA.
FAU - Wing, Rena R
AU  - Wing RR
AD  - Department of Psychiatry and Human Behavior, Alpert Medical School of Brown 
      University, Providence, Rhode Island, USA.
AD  - The Miriam Hospital Weight Control and Diabetes Research Center, Providence, 
      Rhode Island, USA.
FAU - Kelsey, Karl T
AU  - Kelsey KT
AD  - Department of Epidemiology, School of Public Health, Brown University, 
      Providence, Rhode Island, USA.
AD  - Department of Pathology and Laboratory Medicine, Brown University, Providence, 
      Rhode Island, USA.
FAU - McCaffery, Jeanne M
AU  - McCaffery JM
AD  - Department of Psychiatry and Human Behavior, Alpert Medical School of Brown 
      University, Providence, Rhode Island, USA.
AD  - The Miriam Hospital Weight Control and Diabetes Research Center, Providence, 
      Rhode Island, USA.
LA  - eng
GR  - R01 DK066787/DK/NIDDK NIH HHS/United States
GR  - T32HL076134-04/HL/NHLBI NIH HHS/United States
GR  - U01 DK056992/DK/NIDDK NIH HHS/United States
GR  - T32 HL076134/HL/NHLBI NIH HHS/United States
GR  - P50-DA-036107-01/DA/NIDA NIH HHS/United States
GR  - DK066787/DK/NIDDK NIH HHS/United States
GR  - P50 DA036107/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20141218
PL  - England
TA  - Int J Obes (Lond)
JT  - International journal of obesity (2005)
JID - 101256108
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
MH  - Adult
MH  - Body Mass Index
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Caloric Restriction
MH  - DNA Methylation
MH  - *Epigenomics
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*metabolism
MH  - Obesity/genetics/*metabolism/prevention & control/psychology
MH  - Pilot Projects
MH  - *Weight Loss/genetics
PMC - PMC4422763
MID - NIHMS646219
EDAT- 2014/12/19 06:00
MHDA- 2016/03/24 06:00
PMCR- 2015/11/01
CRDT- 2014/12/19 06:00
PHST- 2014/09/10 00:00 [received]
PHST- 2014/11/20 00:00 [revised]
PHST- 2014/11/26 00:00 [accepted]
PHST- 2014/12/19 06:00 [entrez]
PHST- 2014/12/19 06:00 [pubmed]
PHST- 2016/03/24 06:00 [medline]
PHST- 2015/11/01 00:00 [pmc-release]
AID - 10.1038/ijo.2014.213 [doi]
PST - ppublish
SO  - Int J Obes (Lond). 2015 May;39(5):865-868. doi: 10.1038/ijo.2014.213. Epub 2014 
      Dec 18.