PMID- 25521239
OWN - NLM
STAT- MEDLINE
DCOM- 20150831
LR  - 20181113
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 19
IP  - 2
DP  - 2015 Feb
TI  - An integrated assessment of histopathological changes of the enteric 
      neuromuscular compartment in experimental colitis.
PG  - 485-500
LID - 10.1111/jcmm.12428 [doi]
AB  - Bowel inflammatory fibrosis has been largely investigated, but an integrated 
      assessment of remodelling in inflamed colon is lacking. This study evaluated 
      tissue and cellular changes occurring in colonic wall upon induction of colitis, 
      with a focus on neuromuscular compartment. Colitis was elicited in rats by 
      2,4-dinitrobenzenesulfonic acid (DNBS). After 6 and 21 days, the following 
      parameters were assessed on paraffin sections from colonic samples: tissue injury 
      and inflammatory infiltration by histology; collagen and elastic fibres by 
      histochemistry; HuC/D, glial fibrillar acidic protein (GFAP), proliferating cell 
      nuclear antigen (PCNA), nestin, substance P (SP), von Willebrand factor, c-Kit 
      and transmembrane 16A/Anoctamin1 (TMEM16A/ANO1) by immunohistochemistry. 
      TMEM16A/ANO1 was also examined in isolated colonic smooth muscle cells (ICSMCs). 
      On day 6, inflammatory alterations and fibrosis were present in DNBS-treated 
      rats; colonic wall thickening and fibrotic remodelling were evident on day 21. 
      Colitis was associated with both an increase in collagen fibres and a decrease in 
      elastic fibres. Moreover, the neuromuscular compartment of inflamed colon 
      displayed a significant decrease in neuron density and increase in 
      GFAP/PCNA-positive glia of myenteric ganglia, enhanced expression of neural SP, 
      blood vessel remodelling, reduced c-Kit- and TMEM16A/ANO1-positive interstitial 
      cells of Cajal (ICCs), as well as an increase in TMEM16A/ANO1 expression in 
      muscle tissues and ICSMCs. The present findings provide an integrated view of the 
      inflammatory and fibrotic processes occurring in the colonic neuromuscular 
      compartment of rats with DNBS-induced colitis. These morphological alterations 
      may represent a suitable basis for understanding early pathophysiological events 
      related to bowel inflammatory fibrosis.
CI  - (c) 2014 The Authors. Journal of Cellular and Molecular Medicine published by John 
      Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
FAU - Ippolito, Chiara
AU  - Ippolito C
AD  - Unit of Histology, Department of Clinical and Experimental Medicine, University 
      of Pisa, Pisa, Italy.
FAU - Segnani, Cristina
AU  - Segnani C
FAU - Errede, Mariella
AU  - Errede M
FAU - Virgintino, Daniela
AU  - Virgintino D
FAU - Colucci, Rocchina
AU  - Colucci R
FAU - Fornai, Matteo
AU  - Fornai M
FAU - Antonioli, Luca
AU  - Antonioli L
FAU - Blandizzi, Corrado
AU  - Blandizzi C
FAU - Dolfi, Amelio
AU  - Dolfi A
FAU - Bernardini, Nunzia
AU  - Bernardini N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141217
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
SB  - IM
MH  - Animals
MH  - Colitis/*pathology
MH  - Colon/pathology
MH  - Inflammation/pathology
MH  - Male
MH  - Myocytes, Smooth Muscle/*pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC4407593
OTO - NOTNLM
OT  - DNBS
OT  - colonic inflammatory fibrosis
OT  - myenteric ganglia
OT  - neuromuscular compartment
OT  - wall remodelling
EDAT- 2014/12/19 06:00
MHDA- 2015/09/01 06:00
PMCR- 2015/02/01
CRDT- 2014/12/19 06:00
PHST- 2013/07/26 00:00 [received]
PHST- 2014/08/14 00:00 [accepted]
PHST- 2014/12/19 06:00 [entrez]
PHST- 2014/12/19 06:00 [pubmed]
PHST- 2015/09/01 06:00 [medline]
PHST- 2015/02/01 00:00 [pmc-release]
AID - 10.1111/jcmm.12428 [doi]
PST - ppublish
SO  - J Cell Mol Med. 2015 Feb;19(2):485-500. doi: 10.1111/jcmm.12428. Epub 2014 Dec 
      17.