PMID- 25523128
OWN - NLM
STAT- MEDLINE
DCOM- 20160217
LR  - 20191210
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 17
IP  - 7
DP  - 2015 Jul
TI  - In situ DNA-hybridization chain reaction (HCR): a facilitated in situ HCR system 
      for the detection of environmental microorganisms.
PG  - 2532-41
LID - 10.1111/1462-2920.12745 [doi]
AB  - In situ detection of microorganisms by fluorescence in situ hybridization (FISH) 
      is a powerful tool for environmental microbiology, but analyses can be hampered 
      by low rRNA content in target organisms, especially in oligotrophic environments. 
      Here, we present a non-enzymatic, hybridization chain reaction (HCR)-based signal 
      amplified in situ whole-cell detection technique (in situ DNA-HCR). The 
      components of the amplification buffer were optimized to polymerize DNA amplifier 
      probes for in situ DNA-HCR. In situ hybridization of initiator probes followed by 
      signal amplification via HCR produced bright signals with high specificity and 
      probe permeation into cells. The detection rates for Bacteria in a seawater 
      sample and Archaea in anaerobic sludge samples were comparable with or greater 
      than those obtained by catalyzed reporter deposition (CARD)-FISH or standard 
      FISH. Detection of multiple organisms (Bacteria, Archaea and Methanosaetaceae) in 
      an anaerobic sludge sample was achieved by simultaneous in situ DNA-HCR. In 
      summary, in situ DNA-HCR is a simple and easy technique for detecting single 
      microbial cells and enhancing understanding of the ecology and behaviour of 
      environmental microorganisms in situ.
CI  - (c) 2014 Society for Applied Microbiology and John Wiley & Sons Ltd.
FAU - Yamaguchi, Tsuyoshi
AU  - Yamaguchi T
AD  - Department of Environmental Systems Engineering, Nagaoka University of 
      Technology, 1603-1 Kamitomioka, Nagaoka, Niigata, 940-2188, Japan.
FAU - Kawakami, Shuji
AU  - Kawakami S
AD  - Department of Environmental Systems Engineering, Nagaoka University of 
      Technology, 1603-1 Kamitomioka, Nagaoka, Niigata, 940-2188, Japan.
AD  - Department of Construction Systems Engineering, Anan National College of 
      Technology, 265 Aoki Minobayashi, Anan, Tokushima, 774-0017, Japan.
FAU - Hatamoto, Masashi
AU  - Hatamoto M
AD  - Department of Environmental Systems Engineering, Nagaoka University of 
      Technology, 1603-1 Kamitomioka, Nagaoka, Niigata, 940-2188, Japan.
FAU - Imachi, Hiroyuki
AU  - Imachi H
AD  - Department of Subsurface Geobiology Analysis and Research (D-SUGAR), Japan Agency 
      for Marine-Earth Science & Technology (JAMSTEC), Yokosuka, Kanagawa, 237-0061, 
      Japan.
FAU - Takahashi, Masanobu
AU  - Takahashi M
AD  - Department of Environmental Systems Engineering, Nagaoka University of 
      Technology, 1603-1 Kamitomioka, Nagaoka, Niigata, 940-2188, Japan.
AD  - Department of Civil and Environmental Engineering, Tohoku University, 6-6-06 
      Aoba, Sendai, Miyagi, 980-8579, Japan.
FAU - Araki, Nobuo
AU  - Araki N
AD  - Department of Civil Engineering, Nagaoka National College of Technology, 888 
      Nishikatagai, Nagaoka, Niigata, 940-8532, Japan.
FAU - Yamaguchi, Takashi
AU  - Yamaguchi T
AD  - Department of Environmental Systems Engineering, Nagaoka University of 
      Technology, 1603-1 Kamitomioka, Nagaoka, Niigata, 940-2188, Japan.
FAU - Kubota, Kengo
AU  - Kubota K
AD  - Department of Civil and Environmental Engineering, Tohoku University, 6-6-06 
      Aoba, Sendai, Miyagi, 980-8579, Japan.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150211
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Sewage)
SB  - IM
MH  - Archaea/classification/genetics/*isolation & purification
MH  - Bacteria/classification/genetics/*isolation & purification
MH  - DNA, Archaeal/genetics
MH  - DNA, Bacterial/genetics
MH  - Environmental Microbiology
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Methanosarcinales/classification/genetics/*isolation & purification
MH  - Oligonucleotide Probes/genetics
MH  - Seawater/microbiology
MH  - Sensitivity and Specificity
MH  - Sewage/microbiology
EDAT- 2014/12/20 06:00
MHDA- 2016/02/18 06:00
CRDT- 2014/12/20 06:00
PHST- 2014/03/19 00:00 [received]
PHST- 2014/11/22 00:00 [revised]
PHST- 2014/12/06 00:00 [accepted]
PHST- 2014/12/20 06:00 [entrez]
PHST- 2014/12/20 06:00 [pubmed]
PHST- 2016/02/18 06:00 [medline]
AID - 10.1111/1462-2920.12745 [doi]
PST - ppublish
SO  - Environ Microbiol. 2015 Jul;17(7):2532-41. doi: 10.1111/1462-2920.12745. Epub 
      2015 Feb 11.