PMID- 25524212
OWN - NLM
STAT- MEDLINE
DCOM- 20150915
LR  - 20150914
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 185
IP  - 1
DP  - 2015 Jan
TI  - Expression of human leukocyte antigen class I in endocrine and exocrine 
      pancreatic tissue at onset of type 1 diabetes.
PG  - 129-38
AB  - The cause of type 1 diabetes remains unknown. To dissect the link between 
      hyperexpression of human leukocyte antigen (HLA) class I on the islet cells, we 
      examined its expression in subjects with recent-onset type 1 diabetes. IHC showed 
      seemingly pronounced hyperexpression in subjects with recent-onset type 1 
      diabetes, as well as in some nondiabetic subjects. In all subjects, HLA class I 
      expression on exocrine tissue was low. However, no difference in the level of HLA 
      class I expression was found between islet and exocrine tissue using Western 
      blot, flow cytometry, real-time quantitative PCR, or RNA sequencing analyses. 
      Also, the level of HLA class I expression on the messenger level was not 
      increased in islets from subjects with recent-onset type 1 diabetes compared with 
      that in nondiabetic subjects. Consistently, the HLA class I specific enhanceosome 
      (NLRC5) and related transcription factors, as well as interferons, were not 
      enhanced in islets from recent-onset type 1 diabetic subjects. In conclusion, a 
      discrepancy in HLA class I expression in islets assessed by IHC was observed 
      compared with that using quantitative techniques showing similar expression of 
      HLA class I in islets and exocrine tissue in subjects with recent-onset type 1 
      diabetes, nor could any differences be found between type 1 diabetic and 
      nondiabetic subjects. Results presented provide important clues for a better 
      understanding on how this complex disease develops.
FAU - Skog, Oskar
AU  - Skog O
AD  - Department of Immunology, Genetics, and Pathology, Uppsala University, Uppsala, 
      Sweden.
FAU - Korsgren, Stella
AU  - Korsgren S
FAU - Wiberg, Anna
AU  - Wiberg A
FAU - Danielsson, Angelika
AU  - Danielsson A
FAU - Edwin, Bjorn
AU  - Edwin B
FAU - Buanes, Trond
AU  - Buanes T
FAU - Krogvold, Lars
AU  - Krogvold L
FAU - Korsgren, Olle
AU  - Korsgren O
FAU - Dahl-Jorgensen, Knut
AU  - Dahl-Jorgensen K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (NLRC5 protein, human)
RN  - 0 (Transcription Factors)
RN  - 9008-11-1 (Interferons)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child
MH  - Diabetes Mellitus, Type 1/diagnosis/*metabolism
MH  - *Genes, MHC Class I
MH  - Humans
MH  - Interferons/metabolism
MH  - Intracellular Signaling Peptides and Proteins/metabolism
MH  - Islets of Langerhans/*metabolism
MH  - Middle Aged
MH  - Pancreas, Exocrine/*metabolism
MH  - Transcription Factors/metabolism
MH  - Transcriptome
MH  - Young Adult
EDAT- 2014/12/20 06:00
MHDA- 2015/09/16 06:00
CRDT- 2014/12/20 06:00
PHST- 2014/05/15 00:00 [received]
PHST- 2014/08/28 00:00 [revised]
PHST- 2014/09/09 00:00 [accepted]
PHST- 2014/12/20 06:00 [entrez]
PHST- 2014/12/20 06:00 [pubmed]
PHST- 2015/09/16 06:00 [medline]
AID - S0002-9440(14)00548-3 [pii]
AID - 10.1016/j.ajpath.2014.09.004 [doi]
PST - ppublish
SO  - Am J Pathol. 2015 Jan;185(1):129-38. doi: 10.1016/j.ajpath.2014.09.004.