PMID- 25526196
OWN - NLM
STAT- MEDLINE
DCOM- 20151026
LR  - 20141220
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 13
IP  - 4
DP  - 2014 Dec 18
TI  - Chromosome mapping of 18S rDNA and 5S rDNA by dual-color fluorescence in situ 
      hybridization in the half-smooth tongue sole (Cynoglossus semilaevis).
PG  - 10761-8
LID - 10.4238/2014.December.18.17 [doi]
AB  - Half-smooth tongue sole (Cynoglossus semilaevis) is an important aquaculture 
      flatfish in China. Cytogenetic analysis has revealed that its sex determination 
      system is female heterogametic (ZZ/ZW). The W chromosome is morphologically 
      larger and has been considered evolutionarily younger than any other chromosome 
      in the set. However, the genetic origin and evolution process of this 
      neo-chromosome remains unclear. In this study, 2 tandem arrays of rRNA genes were 
      chosen to address this question. Both the major rDNA (18S rDNA) and the minor 
      rDNA (5S rDNA) were located on the C. semilaevis chromosomes by fluorescence in 
      situ hybridization (FISH). Six 18S rDNA signals were observed on the centromeric 
      regions of 3 pairs of autosomes in both males and females. In females, there was 
      an additional 18S rDNA signal mapping to the telomeric region of the W chromosome 
      long arm. With respect to the 5S rDNA, 12 signals were mapped to the centromeric 
      regions of six pairs of autosomes. Two-color FISH further confirmed that the two 
      pairs of the 5S rDNA signals were correspondingly located at the same positions 
      of the same autosomes as those of the 18S rDNA signals. These results allowed us 
      to speculate about the evolution process of the W chromosome. Chromosome fusions 
      and repetitive sequence accumulations might have occurred in C. semilaevis. The 
      synteny and non-synteny of C. semilaevis 18S rDNA and 5S rDNA might imply the 
      original and evolutionary characteristics of this species. These findings will 
      facilitate studies on karyotype evolution of the order Pleuronectiformes.
FAU - Jiang, L
AU  - Jiang L
AD  - Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 
      Ministry of Education, Qingdao, China.
FAU - Jiang, J
AU  - Jiang J
AD  - Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 
      Ministry of Education, Qingdao, China.
FAU - Liu, J
AU  - Liu J
AD  - Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 
      Ministry of Education, Qingdao, China.
FAU - Yuan, J
AU  - Yuan J
AD  - Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 
      Ministry of Education, Qingdao, China.
FAU - Chen, Y
AU  - Chen Y
AD  - Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 
      Ministry of Education, Qingdao, China.
FAU - Zhang, Q
AU  - Zhang Q
AD  - Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 
      Ministry of Education, Qingdao, China.
FAU - Wang, X
AU  - Wang X
AD  - Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 
      Ministry of Education, Qingdao, China wangxubo@ouc.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141218
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Animals
MH  - Chromosome Mapping/methods
MH  - DNA, Ribosomal/*genetics
MH  - Evolution, Molecular
MH  - Female
MH  - Flatfishes/*genetics
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
EDAT- 2014/12/20 06:00
MHDA- 2015/10/27 06:00
CRDT- 2014/12/20 06:00
PHST- 2014/12/20 06:00 [entrez]
PHST- 2014/12/20 06:00 [pubmed]
PHST- 2015/10/27 06:00 [medline]
AID - gmr4223 [pii]
AID - 10.4238/2014.December.18.17 [doi]
PST - epublish
SO  - Genet Mol Res. 2014 Dec 18;13(4):10761-8. doi: 10.4238/2014.December.18.17.