PMID- 25526237
OWN - NLM
STAT- MEDLINE
DCOM- 20151019
LR  - 20191008
IS  - 1556-1380 (Electronic)
IS  - 1556-0864 (Print)
IS  - 1556-0864 (Linking)
VI  - 10
IP  - 2
DP  - 2015 Feb
TI  - Detection of human polyomavirus 7 in human thymic epithelial tumors.
PG  - 360-6
LID - 10.1097/JTO.0000000000000390 [doi]
AB  - INTRODUCTION: Although the molecular genetics possibly underlying the 
      pathogenesis of human thymoma have been extensively studied, its etiology remains 
      poorly understood. Because murine polyomavirus consistently induces thymomas in 
      mice, we assessed the presence of the novel human polyomavirus 7 (HPyV7) in human 
      thymic epithelial tumors. METHODS: HPyV7-DNA Fluorescence in situ hybridization 
      (FISH), DNA-polymerase chain reaction (PCR), and immunohistochemistry (IHC) were 
      performed in 37 thymomas. Of these, 26 were previously diagnosed with myasthenia 
      gravis (MG). In addition, 20 thymic hyperplasias and 20 fetal thymic tissues were 
      tested. RESULTS: HPyV7-FISH revealed specific nuclear hybridization signals 
      within the neoplastic epithelial cells of 23 thymomas (62.2%). With some 
      exceptions, the HPyV7-FISH data correlated with the HPyV7-DNA PCR. By IHC, large 
      T antigen expression of HPyV7 was detected, and double staining confirmed its 
      expression in the neoplastic epithelial cells. Eighteen of the 26 MG-positive and 
      7 of the 11 MG-negative thymomas were HPyV7-positive. Of the 20 hyperplastic 
      thymi, 40% were HPyV7-positive by PCR as confirmed by FISH and IHC in the 
      follicular lymphocytes. All 20 fetal thymi tested HPyV7-negative. CONCLUSIONS: 
      The presence of HPyV7-DNA and large T antigen expression in the majority of 
      thymomas possibly link HPyV7 to human thymomagenesis. Further investigations are 
      needed to elucidate the possible associations of HPyV7 and MG.
FAU - Rennspiess, Dorit
AU  - Rennspiess D
AD  - Departments of *Pathology, daggerCardiothoracic Surgery, and double daggerPulmonology, GROW-School 
      for Oncology and Developmental Biology, Maastricht University Medical Centre, 
      Maastricht, The Netherlands;  section signDepartment of Internal Medicine IV, University 
      Hospital Aachen, Aachen, Germany;  vertical lineLaboratory of Cellular Oncology, National 
      Cancer Institute, Bethesda, Maryland; and  paragraph signDepartment of Neuroscience, Maastricht 
      University, School of Mental Health and Neuroscience (MHeNS), Maastricht, The 
      Netherlands.
FAU - Pujari, Sreedhar
AU  - Pujari S
FAU - Keijzers, Marlies
AU  - Keijzers M
FAU - Abdul-Hamid, Myrurgia A
AU  - Abdul-Hamid MA
FAU - Hochstenbag, Monique
AU  - Hochstenbag M
FAU - Dingemans, Anne-Marie
AU  - Dingemans AM
FAU - Kurz, Anna Kordelia
AU  - Kurz AK
FAU - Speel, Ernst-Jan
AU  - Speel EJ
FAU - Haugg, Anke
AU  - Haugg A
FAU - Pastrana, Diana V
AU  - Pastrana DV
FAU - Buck, Christopher B
AU  - Buck CB
FAU - De Baets, Marc H
AU  - De Baets MH
FAU - Zur Hausen, Axel
AU  - Zur Hausen A
LA  - eng
GR  - Z99 CA999999/Intramural NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - J Thorac Oncol
JT  - Journal of thoracic oncology : official publication of the International 
      Association for the Study of Lung Cancer
JID - 101274235
RN  - Thymic epithelial tumor
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Molecular Biology
MH  - Neoplasms, Glandular and Epithelial/pathology/*virology
MH  - Polyomavirus/genetics/*isolation & purification
MH  - Polyomavirus Infections/pathology/virology
MH  - Thymus Neoplasms/pathology/*virology
MH  - Tumor Virus Infections/pathology/virology
MH  - Young Adult
PMC - PMC4304941
MID - NIHMS631221
COIS- Conflict of interest: The authors declare no conflict of interest.
EDAT- 2014/12/20 06:00
MHDA- 2015/10/20 06:00
PMCR- 2016/02/01
CRDT- 2014/12/20 06:00
PHST- 2014/12/20 06:00 [entrez]
PHST- 2014/12/20 06:00 [pubmed]
PHST- 2015/10/20 06:00 [medline]
PHST- 2016/02/01 00:00 [pmc-release]
AID - S1556-0864(15)32340-6 [pii]
AID - 10.1097/JTO.0000000000000390 [doi]
PST - ppublish
SO  - J Thorac Oncol. 2015 Feb;10(2):360-6. doi: 10.1097/JTO.0000000000000390.