PMID- 25526690
OWN - NLM
STAT- MEDLINE
DCOM- 20160321
LR  - 20150309
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Linking)
VI  - 29
IP  - 3
DP  - 2015 Apr
TI  - Oxidative and pro-inflammatory effects of cobalt and titanium oxide nanoparticles 
      on aortic and venous endothelial cells.
PG  - 426-37
LID - S0887-2333(14)00247-1 [pii]
LID - 10.1016/j.tiv.2014.12.007 [doi]
AB  - Ultra-fine particles have recently been included among the risk factors for the 
      development of endothelium inflammation and atherosclerosis, and cobalt (CoNPs) 
      and titanium oxide nanoparticles (TiNPs) have attracted attention because of 
      their wide range of applications. We investigated their toxicity profiles in two 
      primary endothelial cell lines derived from human aorta (HAECs) and human 
      umbilical vein (HUVECs) by comparing cell viability, oxidative stress, the 
      expression of adhesion molecules and the release of chemokines during NP 
      exposure. Both NPs were very rapidly internalised, and significantly increased 
      adhesion molecule (ICAM-1, VCAM-1, E-selectin) mRNA and protein levels and the 
      release of monocyte chemoattractant protein-1 (MCP-1) and interleukin 8 (IL-8). 
      However, unlike the TiNPs, the CoNPs also induced time- and 
      concentration-dependent metabolic impairment and oxidative stress without any 
      evident signs of cell death or the induction of apoptosis. There were differences 
      between the HAECs and HUVECs in terms of the extent of oxidative stress-related 
      enzyme and vascular adhesion molecule expression, ROS production, and 
      pro-inflammatory cytokine release despite the similar rate of NP internalisation, 
      thus indicating endothelium heterogeneity in response to exogenous stimuli. Our 
      data indicate that NPs can induce endothelial inflammatory responses via various 
      pathways not involving only oxidative stress.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Alinovi, Rossella
AU  - Alinovi R
AD  - Department of Clinical and Experimental Medicine, University of Parma, Italy.
FAU - Goldoni, Matteo
AU  - Goldoni M
AD  - Department of Clinical and Experimental Medicine, University of Parma, Italy. 
      Electronic address: matteo.goldoni@unipr.it.
FAU - Pinelli, Silvana
AU  - Pinelli S
AD  - Department of Clinical and Experimental Medicine, University of Parma, Italy.
FAU - Campanini, Marco
AU  - Campanini M
AD  - IMEM-CNR Institute, Parma, Italy.
FAU - Aliatis, Irene
AU  - Aliatis I
AD  - Department of Physics and Earth Sciences, University of Parma, Italy.
FAU - Bersani, Danilo
AU  - Bersani D
AD  - Department of Physics and Earth Sciences, University of Parma, Italy.
FAU - Lottici, Pier Paolo
AU  - Lottici PP
AD  - Department of Physics and Earth Sciences, University of Parma, Italy.
FAU - Iavicoli, Sergio
AU  - Iavicoli S
AD  - Italian Workers' Compensation Authority (INAIL), Research Area, Department of 
      Occupational Hygiene, Rome, Italy.
FAU - Petyx, Marta
AU  - Petyx M
AD  - Italian Workers' Compensation Authority (INAIL), Research Area, Department of 
      Occupational Hygiene, Rome, Italy.
FAU - Mozzoni, Paola
AU  - Mozzoni P
AD  - Department of Clinical and Experimental Medicine, University of Parma, Italy; 
      Italian Workers' Compensation Authority (INAIL), Research Center at the 
      University of Parma, Italy.
FAU - Mutti, Antonio
AU  - Mutti A
AD  - Department of Clinical and Experimental Medicine, University of Parma, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141216
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 15FIX9V2JP (titanium dioxide)
RN  - 3G0H8C9362 (Cobalt)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Aorta, Thoracic/cytology/drug effects
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cobalt/*toxicity
MH  - Endothelial Cells/*drug effects
MH  - Human Umbilical Vein Endothelial Cells/drug effects
MH  - Humans
MH  - Inflammation/*chemically induced/pathology
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Nanoparticles/*toxicity
MH  - Oxidative Stress/*drug effects
MH  - Particle Size
MH  - Titanium/*toxicity
OTO - NOTNLM
OT  - Cobalt oxide nanoparticles
OT  - Endothelial cells
OT  - Inflammation
OT  - Oxidative stress
OT  - Titanium oxide nanoparticles
EDAT- 2014/12/21 06:00
MHDA- 2016/03/22 06:00
CRDT- 2014/12/21 06:00
PHST- 2014/08/08 00:00 [received]
PHST- 2014/12/05 00:00 [revised]
PHST- 2014/12/08 00:00 [accepted]
PHST- 2014/12/21 06:00 [entrez]
PHST- 2014/12/21 06:00 [pubmed]
PHST- 2016/03/22 06:00 [medline]
AID - S0887-2333(14)00247-1 [pii]
AID - 10.1016/j.tiv.2014.12.007 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2015 Apr;29(3):426-37. doi: 10.1016/j.tiv.2014.12.007. Epub 
      2014 Dec 16.