PMID- 25527973
OWN - NLM
STAT- MEDLINE
DCOM- 20150921
LR  - 20181113
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 20
DP  - 2014 Dec 21
TI  - NF-kappaB signaling pathway confers neuroblastoma cells migration and invasion 
      ability via the regulation of CXCR4.
PG  - 2746-52
LID - 10.12659/MSM.892597 [doi]
AB  - BACKGROUND: Accumulating evidence implicates the transcription factor NF-kappaB as a 
      positive mediator of tumor metastasis, but the molecular mechanism(s) involved in 
      this process remains largely unknown. In this study, we investigated the role of 
      NF-kappaB signaling pathway in the regulation of CXC chemokine receptor-4 (CXCR4) in 
      neuroblastoma metastasis. MATERIAL AND METHODS: NF-kappaB, CXCR4 mRNA and protein 
      expression were measured by RT-PCR, and Western blot. Tumor necrosis factor-alpha 
      (TNF-alpha) was used to induce the upregulation of NF-kappaB and CXCR4. The knockdown of 
      NF-kappaB and CXCR4 was achieved by PDTC. Transwell assay was used to investigate the 
      role of NF-kappaB (P65) in neuroblastoma cell migration and invasion. An in vitro 
      co-culture system was established to investigate the role of tumor 
      microenvironment in regulation of the NF-kappaB signaling pathway. RESULTS: 
      Over-expression of NF-kappaB (p65) promoted tumor migration and invasion through the 
      upregulation of CXCR4; however, knockdown of NF-kappaB(P65) inhibited tumor migration 
      and invasion through blocking the expression of CXCR4. Consistently, in the 
      co-culture system, the expression of CXCR4 was partly dependent on the expression 
      of NF-kappaB (p65). CONCLUSIONS: Our studies reveal critical roles for the NF-kappaB 
      signaling pathway in neuroblastoma migration and invasion. The mechanism may be 
      through up-regulation of CXCR4, mediated by the NF-kappaB signaling pathways. 
      Targeting NF-kappaB signalling pathways and ultimately CXCR4 could be a strategy in 
      neuroblastoma therapy.
FAU - Zhi, Yunlai
AU  - Zhi Y
AD  - Department of Pediatric Surgery, Affiliated Hospital of Qingdao University, 
      Qingdao, Shandong, China (mainland).
FAU - Duan, Yuhe
AU  - Duan Y
AD  - Department of Pediatric Surgery, Affiliated Hospital of Qingdao University, 
      Qingdao, Shandong, China (mainland).
FAU - Zhou, Xianjun
AU  - Zhou X
AD  - Department of Pediatric Surgery, Affiliated Hospital of Qingdao University, 
      Qingdao, Shandong, China (mainland).
FAU - Yin, Xiaofeng
AU  - Yin X
AD  - Department of Neurosurgery, Affiliated Hospital of Medical College, Qingdao 
      University, Qingdao, Shandong, China (mainland).
FAU - Guan, Ge
AU  - Guan G
AD  - Department of Organ Transplantation Center, Affiliated Hospital of Qingdao 
      University, Qingdao, Shandong, China (mainland).
FAU - Zhang, Hong
AU  - Zhang H
AD  - Department of Pediatric Surgery, Affiliated Hospital of Qingdao University, 
      Qingdao, Shandong, China (mainland).
FAU - Dong, Qian
AU  - Dong Q
AD  - Department of Pediatric Surgery, Affiliated Hospital of Qingdao University, 
      Qingdao, Shandong, China (mainland).
FAU - Yang, Kun
AU  - Yang K
AD  - Department of Central Laboratory, Affiliated Hospital of Qingdao University, 
      Qingdao, Shandong, China (mainland).
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141221
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (Chemokine CXCL12)
RN  - 0 (NF-kappa B)
RN  - 0 (Receptors, CXCR4)
SB  - IM
MH  - Brain Neoplasms/metabolism/*pathology
MH  - Cell Line, Tumor
MH  - *Cell Movement/drug effects
MH  - Chemokine CXCL12/pharmacology
MH  - Coculture Techniques
MH  - Humans
MH  - Macrophages/drug effects/metabolism
MH  - NF-kappa B/*metabolism
MH  - Neoplasm Invasiveness
MH  - Neuroblastoma/metabolism/*pathology
MH  - Receptors, CXCR4/*metabolism
MH  - *Signal Transduction/drug effects
MH  - Up-Regulation
PMC - PMC4280060
EDAT- 2014/12/22 06:00
MHDA- 2015/09/22 06:00
PMCR- 2014/12/21
CRDT- 2014/12/22 06:00
PHST- 2014/12/22 06:00 [entrez]
PHST- 2014/12/22 06:00 [pubmed]
PHST- 2015/09/22 06:00 [medline]
PHST- 2014/12/21 00:00 [pmc-release]
AID - 892597 [pii]
AID - 10.12659/MSM.892597 [doi]
PST - epublish
SO  - Med Sci Monit. 2014 Dec 21;20:2746-52. doi: 10.12659/MSM.892597.