PMID- 25529354
OWN - NLM
STAT- MEDLINE
DCOM- 20150409
LR  - 20220321
IS  - 1934-6638 (Electronic)
IS  - 1934-662X (Linking)
VI  - 123
IP  - 2
DP  - 2015 Feb
TI  - Detection of ALK rearrangements in malignant pleural effusion cell blocks from 
      patients with advanced non-small cell lung cancer: a comparison of Ventana 
      immunohistochemistry and fluorescence in situ hybridization.
PG  - 117-22
LID - 10.1002/cncy.21510 [doi]
AB  - BACKGROUND: Surgical resections or tumor biopsies are often not available for 
      patients with late-stage non-small cell lung cancer (NSCLC). Cytological 
      specimens, such as malignant pleural effusion (MPE) cell blocks, are critical for 
      molecular testing. Currently, diagnostic methods to identify anaplastic lymphoma 
      kinase (ALK) rearrangements include fluorescence in situ hybridization (FISH), 
      real-time reverse transcriptase-polymerase chain reaction (RT-PCR), and 
      immunohistochemistry (IHC). In the current study, the authors compared Ventana 
      ALK IHC assays and ALK FISH to detect ALK rearrangements in MPE cell blocks from 
      patients with advanced NSCLC. METHODS: The ALK IHC assay and ALK FISH were 
      performed on 63 MPE cell blocks. RT-PCR analysis was performed as additional 
      validation in cases in which a discrepancy was observed between the IHC assay and 
      FISH results. RESULTS: The Ventana ALK IHC assay was found to be informative for 
      all 63 samples, and 8 cases were positive. Fifty-eight cases were interpretable 
      for FISH detection, and 6 were positive. The concordance between IHC and FISH was 
      100% among the 58 cases. Of the 5 uninterpretable ALK FISH cases, 2 cases and 3 
      cases, respectively, were ALK IHC positive and negative. One of the 2 ALK 
      IHC-positive cases also demonstrated a positive result in the RT-PCR assay and 
      the patient benefited from crizotinib treatment. CONCLUSIONS: MPE cell blocks can 
      be used successfully for the detection of ALK rearrangement when tumor tissue is 
      not available. The Ventana ALK IHC assay is an effective screening method for ALK 
      rearrangement in MPE cell blocks from patients with advanced NSCLC, demonstrating 
      high agreement with FISH results.
CI  - (c) 2014 American Cancer Society.
FAU - Wang, Weiya
AU  - Wang W
AD  - Department of Pathology, West China Hospital of Sichuan University, Chengdu, 
      China.
FAU - Tang, Yuan
AU  - Tang Y
FAU - Li, Jinnan
AU  - Li J
FAU - Jiang, Lili
AU  - Jiang L
FAU - Jiang, Yong
AU  - Jiang Y
FAU - Su, Xueying
AU  - Su X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141219
PL  - United States
TA  - Cancer Cytopathol
JT  - Cancer cytopathology
JID - 101499453
RN  - EC 2.7.10.1 (ALK protein, human)
RN  - EC 2.7.10.1 (Anaplastic Lymphoma Kinase)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Anaplastic Lymphoma Kinase
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*metabolism/pathology
MH  - Pleural Effusion, Malignant/*metabolism
MH  - Receptor Protein-Tyrosine Kinases/*metabolism
MH  - Small Cell Lung Carcinoma/*metabolism/pathology
OTO - NOTNLM
OT  - Ventana immunohistochemistry (IHC) assay
OT  - anaplastic lymphoma kinase (ALK)
OT  - cell block
OT  - fluorescence
OT  - fluorescence in situ hybridization (FISH)
OT  - malignant pleural effusion
OT  - non-small cell lung cancer
EDAT- 2014/12/23 06:00
MHDA- 2015/04/10 06:00
CRDT- 2014/12/23 06:00
PHST- 2014/09/10 00:00 [received]
PHST- 2014/11/20 00:00 [revised]
PHST- 2014/12/01 00:00 [accepted]
PHST- 2014/12/23 06:00 [entrez]
PHST- 2014/12/23 06:00 [pubmed]
PHST- 2015/04/10 06:00 [medline]
AID - 10.1002/cncy.21510 [doi]
PST - ppublish
SO  - Cancer Cytopathol. 2015 Feb;123(2):117-22. doi: 10.1002/cncy.21510. Epub 2014 Dec 
      19.