PMID- 25529677 OWN - NLM STAT- MEDLINE DCOM- 20150806 LR - 20181202 IS - 1873-7072 (Electronic) IS - 0308-8146 (Linking) VI - 174 DP - 2015 May 1 TI - Dynamic behaviour and residual pattern of thiamethoxam and its metabolite clothianidin in Swiss chard using liquid chromatography-tandem mass spectrometry. PG - 248-55 LID - S0308-8146(14)01781-6 [pii] LID - 10.1016/j.foodchem.2014.11.052 [doi] AB - A simultaneous method was developed to analyse thiamethoxam and its metabolite clothianidin in Swiss chard using tandem mass spectrometry (in the positive electrospray ionisation mode using multiple reaction monitoring mode) to estimate the dissipation pattern and the pre-harvest residue limit (PHRL). Thiamethoxam (10%, WG) was sprayed on Swiss chard grown in two different areas under greenhouse conditions at the recommended dose rate of 10 g/20 L water. Samples were collected randomly up to 14 days post-application, extracted using quick, easy, cheap, effective, rugged, safe (QuEChERS) acetate-buffered method and purified via a dispersive solid phase extraction (d-SPE) procedure. Matrix matched calibration showed good linearity with determination coefficients (R(2)) ⩾ 0.998. The limits of detection (LOD) and quantification (LOQ) were 0.007 and 0.02 mg/kg. The method was validated in triplicate at two different spiked concentration levels. Good recoveries (n=3) of 87.48-105.61% with relative standard deviations (RSDs) < 10 were obtained for both analytes. The rate of disappearance of total thiamethoxam residues in/on Swiss chard was best described by first-order kinetics with half-lives of 6.3 and 4.2 days. We predicted from the PHRL curves that if the residues were <19.21 or 26.98 mg/kg at 10 days before harvest, then total thiamethoxam concentrations would be below the maximum residue limits during harvest. CI - Copyright (c) 2014 Elsevier Ltd. All rights reserved. FAU - Rahman, Md Musfiqur AU - Rahman MM AD - Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea. FAU - Farha, Waziha AU - Farha W AD - Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea. FAU - Abd El-Aty, A M AU - Abd El-Aty AM AD - Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea; Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt; Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Republic of Korea. Electronic address: abdelaty44@hotmail.com. FAU - Kabir, Md Humayun AU - Kabir MH AD - Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea. FAU - Im, So Jeong AU - Im SJ AD - Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea. FAU - Jung, Da-I AU - Jung DI AD - Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea. FAU - Choi, Jeong-Heui AU - Choi JH AD - Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea. FAU - Kim, Sung-Woo AU - Kim SW AD - Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea. FAU - Son, Young Wook AU - Son YW AD - Food Standard Division, Ministry of Food and Drug Safety, Chungcheongbuk-do 363-700, Republic of Korea. FAU - Kwon, Chan-Hyeok AU - Kwon CH AD - Food Standard Division, Ministry of Food and Drug Safety, Chungcheongbuk-do 363-700, Republic of Korea. FAU - Shin, Ho-Chul AU - Shin HC AD - Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Republic of Korea. FAU - Shim, Jae-Han AU - Shim JH AD - Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea. Electronic address: jhshim@chonnam.ac.kr. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141114 PL - England TA - Food Chem JT - Food chemistry JID - 7702639 RN - 0 (Guanidines) RN - 0 (Neonicotinoids) RN - 0 (Nitro Compounds) RN - 0 (Oxazines) RN - 0 (Pesticide Residues) RN - 0 (Thiazoles) RN - 2V9906ABKQ (clothianidin) RN - 747IC8B487 (Thiamethoxam) SB - IM MH - Beta vulgaris/*chemistry MH - Chromatography, Liquid/*methods MH - Guanidines/*analysis MH - Neonicotinoids MH - Nitro Compounds/*analysis MH - Oxazines/*analysis MH - Pesticide Residues/*analysis MH - Tandem Mass Spectrometry/*methods MH - Thiamethoxam MH - Thiazoles/*analysis OTO - NOTNLM OT - Clothianidin OT - Dissipation OT - Half-life OT - LC/MS/MS OT - Metabolite OT - Swiss chard OT - Thiamethoxam EDAT- 2014/12/23 06:00 MHDA- 2015/08/08 06:00 CRDT- 2014/12/23 06:00 PHST- 2014/08/06 00:00 [received] PHST- 2014/11/05 00:00 [revised] PHST- 2014/11/08 00:00 [accepted] PHST- 2014/12/23 06:00 [entrez] PHST- 2014/12/23 06:00 [pubmed] PHST- 2015/08/08 06:00 [medline] AID - S0308-8146(14)01781-6 [pii] AID - 10.1016/j.foodchem.2014.11.052 [doi] PST - ppublish SO - Food Chem. 2015 May 1;174:248-55. doi: 10.1016/j.foodchem.2014.11.052. Epub 2014 Nov 14.