PMID- 25529897
OWN - NLM
STAT- MEDLINE
DCOM- 20150903
LR  - 20150105
IS  - 1872-8081 (Electronic)
IS  - 0951-6433 (Linking)
VI  - 40
IP  - 6
DP  - 2014 Nov-Dec
TI  - Chebulagic acid from Terminalia chebula enhances insulin mediated glucose uptake 
      in 3T3-L1 adipocytes via PPARgamma signaling pathway.
PG  - 646-57
LID - 10.1002/biof.1193 [doi]
AB  - The thiazolidinedione (TZDs) class of drugs are very effective for the treatment 
      of type 2 diabetes mellitus (T2DM). But due to the adverse effects of synthetic 
      TZDs, their use is strictly regulated. The therapeutic actions of TZDs are 
      mediated via modulation of peroxisome proliferator-activated receptor gamma 
      (PPARgamma). Naturally occurring PPARgamma modulators are more desirable as they lack the 
      serious adverse effects caused by TZDs. This has prompted the exploitation of 
      medicinal plants used in traditional medicine, for their potential PPARgamma 
      activity. In the present work, we studied chebulagic acid (CHA) isolated from 
      fruits of Terminalia chebula with respect to its effect on adipogenesis, glucose 
      transport, and endocrine function of adipocyte. The mRNA expression profile of 
      PPARgamma target gene CCAAT/enhancer-binding protein alpha (C/EBP-alpha) was analyzed by 
      qRT-PCR. The putative binding mode and the potential ligand-target interactions 
      of CHA, with PPARgamma was analyzed using docking software (Autodock and iGEMDOCKv2). 
      The results showed that CHA enhances PPARgamma signaling and adipogenesis dose 
      dependently but in a moderate way, less than rosiglitazone. GLUT4 expression and 
      adiponectin secretion was increased by CHA treatment. The mRNA expression of 
      PPARgamma target gene C/EBP-alpha was increased in CHA -treated adipocytes. The 
      comparison of results of various parameters of adipogenesis, insulin sensitivity, 
      endocrine function and molecular docking experiments of roziglitazone and 
      chebulagic acid indicate that the latter behaves like partial PPARgamma agonist which 
      could be exploited for phytoceutical development against T2DM.
CI  - (c) 2014 International Union of Biochemistry and Molecular Biology.
FAU - Shyni, Gangadharan Leela
AU  - Shyni GL
AD  - Biochemistry and Cell Culture Laboratory, Agroprocessing and Natural Products 
      Division, Council of Scientific and Industrial Research-National Institute for 
      Interdisciplinary Science and Technology (CSIR-NIIST), Thiruvananthapuram, 
      Kerala, 695019, India.
FAU - Kavitha, Sasidharan
AU  - Kavitha S
FAU - Indu, Sasidharan
AU  - Indu S
FAU - Arya, Anil Das
AU  - Arya AD
FAU - Anusree, Sasidharan Suseela
AU  - Anusree SS
FAU - Vineetha, Vadavanath Prabhakaran
AU  - Vineetha VP
FAU - Vandana, Sankar
AU  - Vandana S
FAU - Sundaresan, Andikannu
AU  - Sundaresan A
FAU - Raghu, Kozhiparambil Gopalan
AU  - Raghu KG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141220
PL  - Netherlands
TA  - Biofactors
JT  - BioFactors (Oxford, England)
JID - 8807441
RN  - 0 (Adiponectin)
RN  - 0 (Adipoq protein, mouse)
RN  - 0 (Benzopyrans)
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (CEBPA protein, mouse)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Glucosides)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
RN  - 0 (PPAR gamma)
RN  - 0 (Plant Extracts)
RN  - 0 (Slc2a4 protein, mouse)
RN  - 0 (Solvents)
RN  - 23094-71-5 (chebulagic acid)
RN  - IY9XDZ35W2 (Glucose)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/cytology/*drug effects/metabolism
MH  - Adipogenesis/drug effects
MH  - Adiponectin/agonists/genetics/metabolism
MH  - Animals
MH  - Benzopyrans/isolation & purification/*pharmacology
MH  - Biological Transport/drug effects
MH  - CCAAT-Enhancer-Binding Proteins/agonists/genetics/metabolism
MH  - Cell Differentiation
MH  - Fruit/chemistry
MH  - Gene Expression Regulation
MH  - Glucose/*metabolism
MH  - Glucose Transporter Type 4/agonists/genetics/metabolism
MH  - Glucosides/isolation & purification/*pharmacology
MH  - Hypoglycemic Agents/isolation & purification/*pharmacology
MH  - Insulin/*metabolism/pharmacology
MH  - Insulin Resistance
MH  - Methanol
MH  - Mice
MH  - Molecular Docking Simulation
MH  - PPAR gamma/*agonists/genetics/metabolism
MH  - Plant Extracts/chemistry
MH  - Signal Transduction
MH  - Solvents
MH  - Terminalia/*chemistry
OTO - NOTNLM
OT  - C/EBP-alpha
OT  - GLUT4
OT  - PPARgamma
OT  - adipogenesis
OT  - adiponectin
OT  - chebulagic acid
OT  - type 2 diabetes
EDAT- 2014/12/23 06:00
MHDA- 2015/09/04 06:00
CRDT- 2014/12/23 06:00
PHST- 2014/09/04 00:00 [received]
PHST- 2014/11/08 00:00 [accepted]
PHST- 2014/12/23 06:00 [entrez]
PHST- 2014/12/23 06:00 [pubmed]
PHST- 2015/09/04 06:00 [medline]
AID - 10.1002/biof.1193 [doi]
PST - ppublish
SO  - Biofactors. 2014 Nov-Dec;40(6):646-57. doi: 10.1002/biof.1193. Epub 2014 Dec 20.