PMID- 25530314
OWN - NLM
STAT- MEDLINE
DCOM- 20150507
LR  - 20150224
IS  - 1528-1167 (Electronic)
IS  - 0013-9580 (Linking)
VI  - 56
IP  - 2
DP  - 2015 Feb
TI  - Large-scale analysis of viral nucleic acid spectrum in temporal lobe epilepsy 
      biopsies.
PG  - 234-43
LID - 10.1111/epi.12890 [doi]
AB  - OBJECTIVE: Chronic inflammatory processes are important promotors of temporal 
      lobe epilepsy (TLE) development. Based on human herpesvirus 6 (HHV-6) DNA 
      detection in brain tissue from patients with TLE, an association of persistent 
      viral infection with TLE has been discussed. Individual studies reported 
      increased HHV-6 DNA in patients with clinical signs of previous inflammatory 
      brain reaction, that is, febrile seizures or meningoencephalitis. However, 
      detection rates vary considerably between different studies. Here we performed a 
      large-scale analysis of viral DNA/RNA spectrum in high-quality TLE biopsies. In 
      addition to all Herpesviridae, we addressed potentially relevant neurotropic RNA 
      viruses. METHODS: DNA and RNA were extracted from 346 fresh-frozen tissue samples 
      removed by epilepsy surgery. Real-time polymerase chain reaction (PCR) and nested 
      PCR were performed for Herpesviridae and RNA viruses, respectively. Clinical data 
      were analyzed for earlier signs of inflammatory brain reactions. Fresh-frozen 
      hippocampal tissue samples from patients without chronic central nervous system 
      (CNS) disease served as controls (n = 62). Seven previous PCR studies with 
      overall 178 TLE patients were additionally analyzed regarding a correlation of 
      clinical parameters and HHV-6 detection. RESULTS: PCR revealed HHV-6B DNA in 34 
      specimens (9.8%) from TLE patients. HHV-6B DNA was also present in eight control 
      samples (12.9%; p > 0.05), but showed a lower virus concentration (p < 0.001). 
      Other herpesviruses and RNA viruses were virtually absent. In patients with 
      clinical signs of previous brain inflammation, HHV-6B DNA was observed in 15.0%, 
      whereas only 6.3% of the samples from patients without febrile seizures or 
      meningoencephalitis were positive for HHV-6B DNA (p < 0.05). A meta-analysis of 
      the eight HHV-6 PCR studies revealed similar results. SIGNIFICANCE: This 
      biopsy-based study shows no differences in frequency of HHV-6B DNA detection 
      between TLE patients and controls. These results do not support the hypothesis of 
      a persistent HHV-6B infection as a major pathogenetic factor in TLE. However, the 
      higher virus load in TLE patients and the increased detection rate of HHV-6B DNA 
      in patients with previous inflammatory brain reactions require further 
      investigations.
CI  - Wiley Periodicals, Inc. (c) 2014 International League Against Epilepsy.
FAU - Esposito, Laura
AU  - Esposito L
AD  - Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany.
FAU - Drexler, Jan F
AU  - Drexler JF
FAU - Braganza, Oliver
AU  - Braganza O
FAU - Doberentz, Elke
AU  - Doberentz E
FAU - Grote, Alexander
AU  - Grote A
FAU - Widman, Guido
AU  - Widman G
FAU - Drosten, Christian
AU  - Drosten C
FAU - Eis-Hubinger, Anna M
AU  - Eis-Hubinger AM
FAU - Schoch, Susanne
AU  - Schoch S
FAU - Elger, Christian E
AU  - Elger CE
FAU - Becker, Albert J
AU  - Becker AJ
FAU - Niehusmann, Pitt
AU  - Niehusmann P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141220
PL  - United States
TA  - Epilepsia
JT  - Epilepsia
JID - 2983306R
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Biopsy
MH  - DNA, Viral/*analysis
MH  - Epilepsy, Temporal Lobe/*pathology/virology
MH  - Female
MH  - Herpesvirus 6, Human/*genetics
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction/methods
MH  - Young Adult
OTO - NOTNLM
OT  - Encephalitis
OT  - Epileptogenesis
OT  - Febrile seizures
OT  - HHV-6
OT  - Inflammation
EDAT- 2014/12/23 06:00
MHDA- 2015/05/08 06:00
CRDT- 2014/12/23 06:00
PHST- 2014/11/10 00:00 [accepted]
PHST- 2014/12/23 06:00 [entrez]
PHST- 2014/12/23 06:00 [pubmed]
PHST- 2015/05/08 06:00 [medline]
AID - 10.1111/epi.12890 [doi]
PST - ppublish
SO  - Epilepsia. 2015 Feb;56(2):234-43. doi: 10.1111/epi.12890. Epub 2014 Dec 20.