PMID- 25531651
OWN - NLM
STAT- MEDLINE
DCOM- 20150826
LR  - 20240314
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 12
DP  - 2014
TI  - Chronic exercise increases plasma brain-derived neurotrophic factor levels, 
      pancreatic islet size, and insulin tolerance in a TrkB-dependent manner.
PG  - e115177
LID - 10.1371/journal.pone.0115177 [doi]
LID - e115177
AB  - BACKGROUND: Physical exercise improves glucose metabolism and insulin 
      sensitivity. Brain-derived neurotrophic factor (BDNF) enhances insulin activity 
      in diabetic rodents. Because physical exercise modifies BDNF production, this 
      study aimed to investigate the effects of chronic exercise on plasma BDNF levels 
      and the possible effects on insulin tolerance modification in healthy rats. 
      METHODS: Wistar rats were divided into five groups: control (sedentary, C); 
      moderate- intensity training (MIT); MIT plus K252A TrkB blocker (MITK); 
      high-intensity training (HIT); and HIT plus K252a (HITK). Training comprised 8 
      weeks of treadmill running. Plasma BDNF levels (ELISA assay), glucose tolerance, 
      insulin tolerance, and immunohistochemistry for insulin and the pancreatic islet 
      area were evaluated in all groups. In addition, Bdnf mRNA expression in the 
      skeletal muscle was measured. PRINCIPAL FINDINGS: Chronic treadmill exercise 
      significantly increased plasma BDNF levels and insulin tolerance, and both 
      effects were attenuated by TrkB blocking. In the MIT and HIT groups, a 
      significant TrkB-dependent pancreatic islet enlargement was observed. MIT rats 
      exhibited increased liver glycogen levels following insulin administration in a 
      TrkB-independent manner. CONCLUSIONS/SIGNIFICANCE: Chronic physical exercise 
      exerted remarkable effects on insulin regulation by inducing significant 
      increases in the pancreatic islet size and insulin sensitivity in a 
      TrkB-dependent manner. A threshold for the induction of BNDF in response to 
      physical exercise exists in certain muscle groups. To the best of our knowledge, 
      these are the first results to reveal a role for TrkB in the chronic 
      exercise-mediated insulin regulation in healthy rats.
FAU - Jimenez-Maldonado, Alberto
AU  - Jimenez-Maldonado A
AD  - Centro Universitario de Investigaciones Biomedicas, Universidad de Colima, 
      Colima, Mexico.
FAU - de Alvarez-Buylla, Elena Roces
AU  - de Alvarez-Buylla ER
AD  - Centro Universitario de Investigaciones Biomedicas, Universidad de Colima, 
      Colima, Mexico.
FAU - Montero, Sergio
AU  - Montero S
AD  - Centro Universitario de Investigaciones Biomedicas, Universidad de Colima, 
      Colima, Mexico.
FAU - Melnikov, Valery
AU  - Melnikov V
AD  - Facultad de Medicina, Universidad de Colima, Colima, Mexico.
FAU - Castro-Rodriguez, Elena
AU  - Castro-Rodriguez E
AD  - Centro Universitario de Investigaciones Biomedicas, Universidad de Colima, 
      Colima, Mexico.
FAU - Gamboa-Dominguez, Armando
AU  - Gamboa-Dominguez A
AD  - Depto de Patologia, Instituto Nacional de Nutricion y Ciencias Medicas "Salvador 
      Zubiran," Mexico City, Mexico D.F.
FAU - Rodriguez-Hernandez, Alejandrina
AU  - Rodriguez-Hernandez A
AD  - Facultad de Medicina, Universidad de Colima, Colima, Mexico.
FAU - Lemus, Monica
AU  - Lemus M
AD  - Centro Universitario de Investigaciones Biomedicas, Universidad de Colima, 
      Colima, Mexico.
FAU - Murguia, Jesus Muniz
AU  - Murguia JM
AD  - Centro Universitario de Investigaciones Biomedicas, Universidad de Colima, 
      Colima, Mexico.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141222
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Insulin)
RN  - 0 (RNA, Messenger)
RN  - 9005-79-2 (Glycogen)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
EIN - PLoS One. 2015;10(3):e0119047. PMID: 25756350
MH  - Animals
MH  - Area Under Curve
MH  - Brain-Derived Neurotrophic Factor/*blood/genetics/metabolism
MH  - Glucose Tolerance Test
MH  - Glycogen/metabolism
MH  - Immunohistochemistry
MH  - Insulin/*metabolism/pharmacology
MH  - Insulin Resistance
MH  - Islets of Langerhans/anatomy & histology/*pathology
MH  - Liver/drug effects/metabolism
MH  - Male
MH  - Muscle, Skeletal/metabolism
MH  - Organ Size
MH  - Physical Conditioning, Animal
MH  - RNA, Messenger/metabolism
MH  - ROC Curve
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkB/antagonists & inhibitors/genetics/*metabolism
PMC - PMC4274083
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/12/23 06:00
MHDA- 2015/08/27 06:00
PMCR- 2014/12/22
CRDT- 2014/12/23 06:00
PHST- 2014/02/19 00:00 [received]
PHST- 2014/11/19 00:00 [accepted]
PHST- 2014/12/23 06:00 [entrez]
PHST- 2014/12/23 06:00 [pubmed]
PHST- 2015/08/27 06:00 [medline]
PHST- 2014/12/22 00:00 [pmc-release]
AID - PONE-D-14-07919 [pii]
AID - 10.1371/journal.pone.0115177 [doi]
PST - epublish
SO  - PLoS One. 2014 Dec 22;9(12):e115177. doi: 10.1371/journal.pone.0115177. 
      eCollection 2014.