PMID- 2553184
OWN - NLM
STAT- MEDLINE
DCOM- 19891219
LR  - 20190510
IS  - 0007-1188 (Print)
IS  - 0007-1188 (Linking)
VI  - 98
IP  - 1
DP  - 1989 Sep
TI  - Intracellular platelet-activating factor regulates eicosanoid generation in 
      guinea-pig resident peritoneal macrophages.
PG  - 141-8
AB  - 1. The role of intracellular platelet-activating factor (Paf) in arachidonic acid 
      (AA) mobilization from guinea-pig peritoneal macrophages has been investigated by 
      use of the potent and selective Paf receptor antagonists, WEB 2086 and CV 6209. 
      2. Adherent macrophages contained cell-associated Paf which was increased by 
      exposure to formyl-methionyl-leucyl-phenylalanine (fMLP), endotoxin and the 
      ionophore, A23187. However, only endotoxin and A23187 caused release of 
      detectable amounts of Paf into the extracellular medium. 3. Exogenous Paf and 
      each of the above stimuli mobilized previously incorporated [14C]-AA and 
      increased the generation of prostacyclin (PGI2) in resident macrophages. 4. WEB 
      2086 (10-100 microM) and CV 6209 (0.1-10 microM) reduced both basal and 
      stimulated PGI2 generation and WEB 2086 inhibited the mobilization of [14C]-AA. 
      In addition, WEB 2086 (10 microM) inhibited fMLP-and Paf-induced superoxide anion 
      generation. Responses to A23187 were not inhibited by either antagonist. 5. 
      Activation of macrophages by fMLP caused a short burst of intracellular Paf 
      generation but none was detected in the supernatants. The time-course of PGI2 
      synthesis followed closely that of Paf. 6. These data suggest that intracellular 
      Paf generation is important for subsequent AA mobilization and may have a wider 
      role in signal transduction processes.
FAU - Stewart, A G
AU  - Stewart AG
AD  - Department of Physiology, University of Melbourne, Victoria, Australia.
FAU - Phillips, W A
AU  - Phillips WA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Pharmacol
JT  - British journal of pharmacology
JID - 7502536
RN  - 0 (Arachidonic Acids)
RN  - 0 (Azepines)
RN  - 0 (Culture Media)
RN  - 0 (Eicosanoids)
RN  - 0 (Platelet Activating Factor)
RN  - 0 (Triazines)
RN  - 0 (Triazoles)
RN  - 105219-56-5 (WEB 2086)
RN  - 11062-77-4 (Superoxides)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 58962-34-8 (6-Ketoprostaglandin F1 alpha)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - 6-Ketoprostaglandin F1 alpha/biosynthesis
MH  - Animals
MH  - Arachidonic Acid
MH  - Arachidonic Acids/*metabolism
MH  - Azepines/pharmacology
MH  - Calcimycin/pharmacology
MH  - Chromatography, High Pressure Liquid
MH  - Culture Media
MH  - Dinoprostone/biosynthesis
MH  - Eicosanoids/*biosynthesis
MH  - Guinea Pigs
MH  - In Vitro Techniques
MH  - Macrophages/drug effects/*metabolism
MH  - Male
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Peritoneal Cavity/cytology
MH  - Platelet Activating Factor/antagonists & inhibitors/biosynthesis/*physiology
MH  - Superoxides/metabolism
MH  - Triazines/pharmacology
MH  - *Triazoles
PMC - PMC1854646
EDAT- 1989/09/01 00:00
MHDA- 1989/09/01 00:01
PMCR- 1990/09/01
CRDT- 1989/09/01 00:00
PHST- 1989/09/01 00:00 [pubmed]
PHST- 1989/09/01 00:01 [medline]
PHST- 1989/09/01 00:00 [entrez]
PHST- 1990/09/01 00:00 [pmc-release]
AID - 10.1111/j.1476-5381.1989.tb16874.x [doi]
PST - ppublish
SO  - Br J Pharmacol. 1989 Sep;98(1):141-8. doi: 10.1111/j.1476-5381.1989.tb16874.x.