PMID- 25533544
OWN - NLM
STAT- MEDLINE
DCOM- 20150521
LR  - 20171116
IS  - 1096-0945 (Electronic)
IS  - 0014-4800 (Linking)
VI  - 98
IP  - 1
DP  - 2015 Feb
TI  - Vacuolar protein sorting 4B regulates apoptosis of intestinal epithelial cells 
      via p38 MAPK in Crohn's disease.
PG  - 55-64
LID - S0014-4800(14)00204-4 [pii]
LID - 10.1016/j.yexmp.2014.12.007 [doi]
AB  - Vacuolar protein sorting 4B (VPS4B), a member of ATPase family proteins, 
      reportedly possesses multiple biological functions, such as regulating the 
      development of breast cancer and non-small-cell lung cancer, participating in 
      Parkinson's disease, and modulating neuronal apoptosis after cerebral ischemia. 
      However, its expression and potential functions in Crohn's disease (CD) has not 
      been understood. In this study, we reported for the first time that VPS4B was 
      over-expressed in intestinal epithelial cell (IECs) of patients with CD. In 
      TNBS-induced mouse colitis models, we observed the up-regulation of VPS4B was 
      accompanied with the elevated levels of IEC apoptotic markers (active caspase-3 
      and cleaved PARP) and phosphorylated p38 in colitis IECs. Co-localization of 
      VPS4B and active caspase-3 in IECs of the TNBS group further indicated the 
      possible involvement of VPS4B in IEC apoptosis. Employing the TNF-alpha-treated HT29 
      cells as an in vitro IEC apoptosis model, we confirmed the positive correlation 
      of VPS4B with caspase-dependent cellular apoptosis. Knocking VPS4B down by siRNA 
      significantly alleviated TNF-alpha-induced p38 phosphorylation and cellular apoptosis 
      in HT29 cells. Taken together, our findings suggested that VPS4B may facilitate 
      the IEC apoptosis in CD via p38 MAPK signaling pathway.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Zhang, Dongmei
AU  - Zhang D
AD  - Department of Pathogen Biology, Medical College, Jiangsu Province Key Laboratory 
      for Inflammation and Molecular Drug Target, Nantong University, 19 Qixiu Road, 
      Nantong 226001, Jiangsu Province, People's Republic of China.
FAU - Wang, Liang
AU  - Wang L
AD  - Department of Gastroenterology, Affiliated Hospital of Nantong University, 20 
      Xisi Road, Nantong 226001, Jiangsu Province, People's Republic of China.
FAU - Yan, Lijun
AU  - Yan L
AD  - Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical 
      University, 300 Guangzhou Road, Nanjing 210000, Jiangsu Province, People's 
      Republic of China.
FAU - Miao, Xianjing
AU  - Miao X
AD  - Department of Gastroenterology, Affiliated Hospital of Nantong University, 20 
      Xisi Road, Nantong 226001, Jiangsu Province, People's Republic of China.
FAU - Gong, Chen
AU  - Gong C
AD  - Department of Gastroenterology, Affiliated Hospital of Nantong University, 20 
      Xisi Road, Nantong 226001, Jiangsu Province, People's Republic of China.
FAU - Xiao, Min
AU  - Xiao M
AD  - National Glycoengineering Research Center, State Key Laboratory of Microbial 
      Technology, Shandong University, Jinan 250100, People's Republic of China.
FAU - Ni, Runzhou
AU  - Ni R
AD  - Department of Gastroenterology, Affiliated Hospital of Nantong University, 20 
      Xisi Road, Nantong 226001, Jiangsu Province, People's Republic of China.
FAU - Tang, Qiyun
AU  - Tang Q
AD  - Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical 
      University, 300 Guangzhou Road, Nanjing 210000, Jiangsu Province, People's 
      Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141220
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.4.- (ATPases Associated with Diverse Cellular Activities)
RN  - EC 3.6.4.6 (VPS4B protein, human)
SB  - IM
MH  - ATPases Associated with Diverse Cellular Activities
MH  - Adenosine Triphosphatases/antagonists & inhibitors/genetics/*metabolism
MH  - Animals
MH  - *Apoptosis
MH  - Blotting, Western
MH  - Caspase 3/metabolism
MH  - Cell Proliferation
MH  - Colitis/chemically induced/metabolism/pathology
MH  - Crohn Disease/drug therapy/metabolism/*pathology
MH  - Endosomal Sorting Complexes Required for Transport/antagonists & 
      inhibitors/genetics/*metabolism
MH  - Epithelial Cells/drug effects/metabolism/*pathology
MH  - Female
MH  - HT29 Cells
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Intestinal Mucosa/drug effects/metabolism/*pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - RNA, Small Interfering/genetics
MH  - Signal Transduction
MH  - Trinitrobenzenesulfonic Acid/toxicity
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - p38 Mitogen-Activated Protein Kinases/*metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Crohn's disease
OT  - Intestinal epithelial cell
OT  - Phosphorylated p38
OT  - VPS4B
EDAT- 2014/12/24 06:00
MHDA- 2015/05/23 06:00
CRDT- 2014/12/24 06:00
PHST- 2014/12/15 00:00 [received]
PHST- 2014/12/18 00:00 [accepted]
PHST- 2014/12/24 06:00 [entrez]
PHST- 2014/12/24 06:00 [pubmed]
PHST- 2015/05/23 06:00 [medline]
AID - S0014-4800(14)00204-4 [pii]
AID - 10.1016/j.yexmp.2014.12.007 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2015 Feb;98(1):55-64. doi: 10.1016/j.yexmp.2014.12.007. Epub 2014 
      Dec 20.