PMID- 25533996 OWN - NLM STAT- MEDLINE DCOM- 20151001 LR - 20150202 IS - 1095-9947 (Electronic) IS - 1050-4648 (Linking) VI - 43 IP - 1 DP - 2015 Mar TI - Analysis of the transcriptomic profilings of Mandarin fish (Siniperca chuatsi) infected with Flavobacterium columnare with an emphasis on immune responses. PG - 111-9 LID - S1050-4648(14)00477-X [pii] LID - 10.1016/j.fsi.2014.12.006 [doi] AB - Flavobacterium columnare (FC) is the causative pathogen of columnaris which has caused great economic loss in fish culture worldwide, including in Mandarin fish (Siniperca chuatsi) culture. In the present study, the transcriptomic profiles of the head kidneys from FC-infected and non-infected Mandarin fish were obtained using HiSeq 2000 (Illumina). Totally 31,168 unigenes with high quality were obtained. Genes involved in protein folding, metabolism and energy, immune responses, oxidoreductase activity, cell growth and death were identified as enriched classes. 1019 differently expressed genes between the two groups were identified, including 603 up-regulated and 416 down-regulated genes. 27 differently expressed immune related genes were scrutinized, including 17 up-regulated and 10 down-regulated genes. Six of the differently expressed genes were further validated by qRT-PCR. The roles of the immune related genes were discussed. Identification of the host genes in response to FC infection will shed a new light on the prevention of columnaris. CI - Copyright (c) 2014 Elsevier Ltd. All rights reserved. FAU - Zhou, Weidong AU - Zhou W AD - Wuhan Fishery Research Institute, 430207 Wuhan, Hubei, China. FAU - Zhang, Yulei AU - Zhang Y AD - Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural University, 430070 Wuhan, Hubei, China; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, 430070 Wuhan, Hubei, China. FAU - Wen, Yi AU - Wen Y AD - Division of Science and Technology, Beijing Normal University - Hong Kong Baptist University United International College, 519085 Zhuhai, Guangdong, China. FAU - Ji, Wei AU - Ji W AD - Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural University, 430070 Wuhan, Hubei, China; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, 430070 Wuhan, Hubei, China. FAU - Zhou, Yang AU - Zhou Y AD - Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, 430070 Wuhan, Hubei, China. FAU - Ji, Yachan AU - Ji Y AD - Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, 430070 Wuhan, Hubei, China. FAU - Liu, Xiaoling AU - Liu X AD - Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, 430070 Wuhan, Hubei, China. FAU - Wang, Weimin AU - Wang W AD - China Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, 430070 Wuhan, Hubei, China. FAU - Asim, Muhammad AU - Asim M AD - Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural University, 430070 Wuhan, Hubei, China; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, 430070 Wuhan, Hubei, China. FAU - Liang, Xufang AU - Liang X AD - Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural University, 430070 Wuhan, Hubei, China; China Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, 430070 Wuhan, Hubei, China. Electronic address: xfliang@mail.hzau.edu.cn. FAU - Ai, Taoshan AU - Ai T AD - Wuhan Fishery Research Institute, 430207 Wuhan, Hubei, China. FAU - Lin, Li AU - Lin L AD - Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural University, 430070 Wuhan, Hubei, China; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, 430070 Wuhan, Hubei, China; China Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, 430070 Wuhan, Hubei, China. Electronic address: linli@mail.hzau.edu.cn. LA - eng SI - GENBANK/KP121415 SI - GENBANK/KP121416 SI - GENBANK/KP121417 SI - GENBANK/KP121418 SI - GENBANK/KP121419 SI - GENBANK/KP121420 SI - GENBANK/KP121421 SI - GENBANK/KP121422 SI - GENBANK/KP121423 SI - GENBANK/KP121424 SI - GENBANK/KP121425 SI - GENBANK/KP121426 SI - GENBANK/KP121427 SI - GENBANK/KP121428 SI - GENBANK/KP121429 SI - GENBANK/KP121430 SI - GENBANK/KP121431 SI - GENBANK/KP121432 SI - GENBANK/KP121433 SI - GENBANK/KP121434 SI - GENBANK/KP121435 SI - GENBANK/KP121436 SI - GENBANK/KP121437 SI - GENBANK/KP121438 SI - GENBANK/KP121439 SI - GENBANK/KP121440 SI - GENBANK/KP121441 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141219 PL - England TA - Fish Shellfish Immunol JT - Fish & shellfish immunology JID - 9505220 RN - 0 (Fish Proteins) SB - IM MH - Animals MH - Fish Diseases/*immunology/microbiology MH - Fish Proteins/*genetics/metabolism MH - Flavobacteriaceae Infections/immunology/microbiology/*veterinary MH - Flavobacterium/*physiology MH - *Immunity, Innate MH - Molecular Sequence Data MH - *Perciformes MH - Sequence Analysis, DNA/veterinary MH - Transcriptome OTO - NOTNLM OT - Differently expressed genes OT - Flavobacterium columnare OT - Immune responses OT - Siniperca chuatsi OT - Transcriptome EDAT- 2014/12/24 06:00 MHDA- 2015/10/02 06:00 CRDT- 2014/12/24 06:00 PHST- 2014/09/13 00:00 [received] PHST- 2014/11/20 00:00 [revised] PHST- 2014/12/11 00:00 [accepted] PHST- 2014/12/24 06:00 [entrez] PHST- 2014/12/24 06:00 [pubmed] PHST- 2015/10/02 06:00 [medline] AID - S1050-4648(14)00477-X [pii] AID - 10.1016/j.fsi.2014.12.006 [doi] PST - ppublish SO - Fish Shellfish Immunol. 2015 Mar;43(1):111-9. doi: 10.1016/j.fsi.2014.12.006. Epub 2014 Dec 19.