PMID- 2553491
OWN - NLM
STAT- MEDLINE
DCOM- 19891221
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 257
IP  - 1
DP  - 1989 Oct 23
TI  - Granulocyte/macrophage colony-stimulating factor primes human neutrophils for 
      increased diacylglycerol generation in response to chemoattractant.
PG  - 188-90
AB  - Pretreatment of human neutrophils with granulocyte macrophage-colony stimulating 
      factor (GM-CSF) augments several biological responses to chemoattractants (e.g. 
      the respiratory burst, degranulation, and chemotaxis). However, little is known 
      regarding the intracellular effects of priming with GM-CSF. In the present study, 
      we have investigated the effects of GM-CSF on the generation of diacylglycerol 
      (DAG), a proposed mediator of neutrophil responses. GM-CSF alone produced only a 
      small increase in cellular DAG mass, which was most apparent after 30 min. GM-CSF 
      pretreatment (60 min), however, caused a striking augmentation in DAG generation 
      in response to the chemoattractant formyl-methionyl-leucyl-phenylalanine (fMLP), 
      compared with neutrophils preincubated without GM-CSF. The augmentation in DAG 
      generation correlated with an enhancement by GM-CSF of superoxide generation in 
      response to fMLP. The data suggest that GM-CSF may exert some of its biological 
      effects by enhancing DAG generation in response to a second agonist.
FAU - Tyagi, S R
AU  - Tyagi SR
AD  - Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 
      30322.
FAU - Winton, E F
AU  - Winton EF
FAU - Lambeth, J D
AU  - Lambeth JD
LA  - eng
GR  - CA46508/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Colony-Stimulating Factors)
RN  - 0 (Diglycerides)
RN  - 0 (Glycerides)
RN  - 0 (Growth Substances)
RN  - 11062-77-4 (Superoxides)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Chemotaxis, Leukocyte/*drug effects
MH  - Colony-Stimulating Factors/*pharmacology
MH  - Diglycerides/*blood
MH  - Glycerides/*blood
MH  - Granulocyte-Macrophage Colony-Stimulating Factor
MH  - Growth Substances/*pharmacology
MH  - Humans
MH  - In Vitro Techniques
MH  - Kinetics
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Neutrophils/drug effects/metabolism/*physiology
MH  - Superoxides/blood
EDAT- 1989/10/23 00:00
MHDA- 1989/10/23 00:01
CRDT- 1989/10/23 00:00
PHST- 1989/10/23 00:00 [pubmed]
PHST- 1989/10/23 00:01 [medline]
PHST- 1989/10/23 00:00 [entrez]
AID - 0014-5793(89)81817-4 [pii]
AID - 10.1016/0014-5793(89)81817-4 [doi]
PST - ppublish
SO  - FEBS Lett. 1989 Oct 23;257(1):188-90. doi: 10.1016/0014-5793(89)81817-4.