PMID- 25535832
OWN - NLM
STAT- MEDLINE
DCOM- 20150429
LR  - 20150221
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Linking)
VI  - 156
IP  - 3
DP  - 2015 Mar
TI  - Acyl ghrelin acts in the brain to control liver function and peripheral glucose 
      homeostasis in male mice.
PG  - 858-68
LID - 10.1210/en.2014-1733 [doi]
AB  - Recent evidence suggests that peripheral ghrelin regulates glucose metabolism. 
      Here, we designed experiments to examine how central acyl ghrelin infusion 
      affects peripheral glucose metabolism under pair-fed or ad libitum feeding 
      conditions. Mice received intracerebroventricular (icv) infusion of artificial 
      cerebrospinal fluid (aCSF), ghrelin, and allowed to eat ad libitum (icv ghrelin 
      ad lib) or ghrelin and pair-fed to the aCSF group (icv ghrelin pf). Minipumps 
      delivered acyl ghrelin at a dose of 0.25 mug/h at 0.5 muL/h for 7 days. There was 
      no difference in daily blood glucose, insulin, glucagon, triglycerides, or 
      nonesterified fatty acids. Body weight gain and food intake was significantly 
      higher in icv ghrelin ad lib mice. However, both icv ghrelin ad lib and icv 
      ghrelin pf groups exhibited heavier white adipose mass. Icv ghrelin pf mice 
      exhibited better glucose tolerance than aCSF or icv ghrelin ad lib mice during a 
      glucose tolerance test, although both icv ghrelin ad lib and icv ghrelin pf 
      increased insulin release during the glucose tolerance test. Central acyl ghrelin 
      infusion and pair feeding also increased breakdown of liver glycogen and 
      triglyceride, and regulated genes involved in hepatic lipid and glucose 
      metabolism. Icv ghrelin pf mice had an increase in plasma blood glucose during a 
      pyruvate tolerance test relative to icv ghrelin ad lib or aCSF mice. Our results 
      suggest that under conditions of negative energy (icv ghrelin pf), central acyl 
      ghrelin engages a neural circuit that influences hepatic glucose function. 
      Metabolic status affects the ability of central acyl ghrelin to regulate 
      peripheral glucose homeostasis.
FAU - Stark, Romana
AU  - Stark R
AD  - Department of Physiology (R.S., A.R., S.H.L., Q.W., Z.B.A.), Monash University, 
      Clayton, Victoria 3800, Australia; Traditional Chinese Medicine Department 
      (Q.W.), Peking Union Medical College Hospital, Dongcheng District, Beijing 
      100730, China; Department of Animal Physiology (C.P., A.T.), Faculty of Biology, 
      Phillips University, D-35043 Marburg, Germany; and Department of Physiology 
      (A.T.), Otago School of Medical Sciences, University of Otago, Dunedin 9054, New 
      Zealand.
FAU - Reichenbach, Alex
AU  - Reichenbach A
FAU - Lockie, Sarah H
AU  - Lockie SH
FAU - Pracht, Corinna
AU  - Pracht C
FAU - Wu, Qunli
AU  - Wu Q
FAU - Tups, Alexander
AU  - Tups A
FAU - Andrews, Zane B
AU  - Andrews ZB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141223
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Blood Glucose)
RN  - 0 (Ghrelin)
RN  - 0 (Insulin)
RN  - 0 (Leptin)
RN  - 8558G7RUTR (Pyruvic Acid)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Acylation
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Ghrelin/blood/*pharmacology
MH  - Glucose/*metabolism
MH  - Glucose Tolerance Test
MH  - Homeostasis/*drug effects
MH  - Insulin/blood/metabolism
MH  - Leptin/blood/metabolism
MH  - Liver/*drug effects/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Pyruvic Acid/metabolism
EDAT- 2014/12/24 06:00
MHDA- 2015/04/30 06:00
CRDT- 2014/12/24 06:00
PHST- 2014/12/24 06:00 [entrez]
PHST- 2014/12/24 06:00 [pubmed]
PHST- 2015/04/30 06:00 [medline]
AID - 10.1210/en.2014-1733 [doi]
PST - ppublish
SO  - Endocrinology. 2015 Mar;156(3):858-68. doi: 10.1210/en.2014-1733. Epub 2014 Dec 
      23.