PMID- 25536319
OWN - NLM
STAT- MEDLINE
DCOM- 20160307
LR  - 20211203
IS  - 1802-9973 (Electronic)
IS  - 0862-8408 (Linking)
VI  - 64
IP  - 3
DP  - 2015
TI  - Activation of Nrf2 by ischemic preconditioning and sulforaphane in renal 
      ischemia/reperfusion injury: a comparative experimental study.
PG  - 313-23
AB  - Objectives of the study were to investigate impact of ischemic preconditioning 
      (Ipre) and sulforaphane (SFN) and combination of them on nuclear factor 2 
      erythroid related factor 2 (Nrf2) gene and its dependent genes, heme oxygenase-1 
      (HO1) and NADPH-quinone oxidoreductase1 (NQO-1) and inflammatory cytokines 
      TNF-alpha, IL1beta, and intercellular adhesion molecule-1 (ICAM1) and caspase-3 
      in renal ischemia/reperfusion (I/R) injury. Ninety male Sprague Dawely rats were 
      classified into 5 groups (each consists of 18 rats): sham, control, Ipre, 
      sulforaphane and Sulfo+Ipre. Each group was subdivided into 3 subgroups each 
      containing 6 rats according to time of harvesting kidney and taking blood 
      samples; 24 h, 48 h, and 7 days subgroups. Renal functions including serum 
      creatinine, BUN were measured at basal conditions and by the end of experiment. 
      Expression of Nrf2, HO-1, NQO-1, TNF-alpha, IL-1beta, and ICAM-1 was measured by 
      real time PCR in kidney tissues by the end of experiment. Also, 
      immunohistochemical localization of caspase-3 and chemical assay of 
      malondialdehyde (MDA), GSH and SOD activity were measured in kidney tissues. Both 
      Ipre and SFN improved kidney functions, enhanced the expression of Nrf2, HO-1, 
      and NQO-1, attenuated the expression of inflammatory (TNF-alpha, IL-1, and 
      ICAM-1) and apoptotic (caspase-3) markers. However, the effect of sulforaphane 
      was more powerful than Ipre. Also, a combination of them caused more improvement 
      in antioxidant genes expression and more attenuation in inflammatory genes but 
      not caspase-3 than each one did separately. Sulforaphane showed more powerful 
      effect in renoprotection against I/R injury than Ipre as well as there might be a 
      synergism between them at the molecular but not at the function level.
FAU - Shokeir, A A
AU  - Shokeir AA
AD  - Urology and Nephrology Center, Mansoura University and Physiology Department, 
      Faculty of Medicine, Mansoura University, Mansoura, Egypt. zizomenna28@yahoo.com; 
      menhag@mans.edu.eg.
FAU - Barakat, N
AU  - Barakat N
FAU - Hussein, A M
AU  - Hussein AM
FAU - Awadalla, A
AU  - Awadalla A
FAU - Harraz, A M
AU  - Harraz AM
FAU - Khater, S
AU  - Khater S
FAU - Hemmaid, K
AU  - Hemmaid K
FAU - Kamal, A I
AU  - Kamal AI
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141222
PL  - Czech Republic
TA  - Physiol Res
JT  - Physiological research
JID - 9112413
RN  - 0 (Cytokines)
RN  - 0 (Isothiocyanates)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, rat)
RN  - 0 (Sulfoxides)
RN  - GA49J4310U (sulforaphane)
SB  - IM
MH  - Acute Kidney Injury/diagnosis/*immunology/*therapy
MH  - Animals
MH  - Combined Modality Therapy
MH  - Cytokines/immunology
MH  - Ischemic Preconditioning/*methods
MH  - Isothiocyanates/*administration & dosage
MH  - Male
MH  - NF-E2-Related Factor 2/*immunology
MH  - Oxidative Stress/drug effects/immunology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/diagnosis/*immunology/therapy
MH  - Sulfoxides
MH  - Treatment Outcome
EDAT- 2014/12/24 06:00
MHDA- 2016/03/08 06:00
CRDT- 2014/12/24 06:00
PHST- 2014/12/24 06:00 [entrez]
PHST- 2014/12/24 06:00 [pubmed]
PHST- 2016/03/08 06:00 [medline]
AID - 932834 [pii]
AID - 10.33549/physiolres.932834 [doi]
PST - ppublish
SO  - Physiol Res. 2015;64(3):313-23. doi: 10.33549/physiolres.932834. Epub 2014 Dec 
      22.