PMID- 2553710
OWN - NLM
STAT- MEDLINE
DCOM- 19891128
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 264
IP  - 31
DP  - 1989 Nov 5
TI  - Mass changes in inositol tetrakis- and pentakisphosphate isomers induced by 
      chemotactic peptide stimulation in HL-60 cells.
PG  - 18489-93
AB  - Absolute concentrations of inositol phosphate isomers (InsP(s] were quantified in 
      the myeloid cell line HL-60 using the metal-dye detection technique. Stimulation 
      with the chemotactic peptide formyl-methionyl-leucyl-phenylalanine (fMLP) led to 
      distinct alterations in at least seven different inositol phosphate species. 
      Whereas the intracellular concentrations of the tetrakisphosphate isomers 
      (InsP4(s] were found below the micromolar range, inositol 1,3,4,5,6-pentakis- and 
      hexakisphosphate levels were about two orders of magnitude higher (36 and 54 +/- 
      2 microM (mean +/- S.D.), respectively). The three InsP4(s) showed distinct 
      kinetic pattern upon receptor activation, the transient elevation of inositol 
      1,3,4,5-tetrakisphosphate being faster both in onset and in redecrease than 
      inositol 1,3,4,6-tetrakisphosphate. Whereas the two latter isomers reached 
      maximally 2.75 and 2.9 +/- 0.2 microM, respectively, 1 min after stimulation, 
      inositol 3,4,5,6-tetrakisphosphate remained elevated (3.5 +/- 0.4 microM) up to 5 
      min after fMLP. Unexpected changes in highly phosphorylated InsP(s) were 
      observed, notably a rise in inositol 1,3,4,5,6-pentakisphosphate and in inositol 
      hexakisphosphate to 52 +/- 3 and 60 +/- 1 microM, respectively. In terms of mass, 
      the increases in highly phosphorylated inositols are by far highest among all 
      InsP(s). Combining radiotracer method with mass determination it was observed 
      that the specific radioactivity of various InsP(s) was different and changed 
      markedly upon fMLP stimulation, in spite of a prolonged labeling period leading 
      to apparent isotopic steady state. The data presented demonstrate agonist-induced 
      elevations of highly phosphorylated InsP(s) and suggest that inositol 
      1,4,5-trisphosphate, product of receptor-activated phospholipase C, is 
      metabolized rather via phosphorylation than only by dephosphorylation pathways.
FAU - Pittet, D
AU  - Pittet D
AD  - Infectious Diseases Division, University Hospital, Geneve, Switzerland.
FAU - Schlegel, W
AU  - Schlegel W
FAU - Lew, D P
AU  - Lew DP
FAU - Monod, A
AU  - Monod A
FAU - Mayr, G W
AU  - Mayr GW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Inositol Phosphates)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Resorcinols)
RN  - 102850-29-3 (inositol-1,3,4,5-tetrakisphosphate)
RN  - 110298-84-5 (inositol-1,3,4,6-tetrakisphosphate)
RN  - 112791-61-4 (inositol-3,4,5,6-tetrakisphosphate)
RN  - 25663-09-6 (inositol pentaphosphate)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - N68T40H95T (4-(2-pyridylazo)resorcinol)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Granulocytes/metabolism
MH  - Humans
MH  - Inositol Phosphates/analysis/*metabolism
MH  - Isomerism
MH  - Kinetics
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Phosphorylation
MH  - Receptors, Cell Surface/physiology
MH  - Resorcinols
MH  - Tumor Cells, Cultured
EDAT- 1989/11/05 00:00
MHDA- 1989/11/05 00:01
CRDT- 1989/11/05 00:00
PHST- 1989/11/05 00:00 [pubmed]
PHST- 1989/11/05 00:01 [medline]
PHST- 1989/11/05 00:00 [entrez]
AID - S0021-9258(18)51493-9 [pii]
PST - ppublish
SO  - J Biol Chem. 1989 Nov 5;264(31):18489-93.