PMID- 25538065
OWN - NLM
STAT- MEDLINE
DCOM- 20150921
LR  - 20181202
IS  - 1878-3619 (Electronic)
IS  - 0959-2989 (Linking)
VI  - 25
IP  - 1 Suppl
DP  - 2015
TI  - Differentiation of human umbilical cord-derived mesenchymal stem cells into 
      hepatocytes in vitro.
PG  - 145-57
LID - 10.3233/BME-141249 [doi]
AB  - OBJECTIVE: The stem cell based therapy is a potential alternative to liver 
      transplantation. The aim of the study is to investigate the hepatocytic 
      differentiation ability of human umbilical cord derived mesenchymal stem cells 
      (HUC-MSCs) in vitro. METHODS: The HUC-MSCs were isolated from Wharton's jelly of 
      human umbilical cord. The cells were identified by assessing the stem cell 
      markers. The HUC-MSCs were characterized by multipotency of differentiation. We 
      modified the hepatogenic differentiation protocol and examine the function of 
      differentiated cell by Periodic acid-Schiff (PAS) staining and Low-Density 
      Lipoprotein (LDL) uptake. The protein expressions of Total protein (TP), Albumin 
      (ALB), Globulin (GLB), Urea (BUN) and alpha-fetoprotein (AFP) were also detected. 
      RESULTS: The cells of the hepatogenic differentiation group showed the function 
      of hepatocyte. Protein expressions of TP, ALB, GLB BUN and AFP improve that the 
      HUC-MSCs are able to different into the functional hepatogenic-like cell. 
      CONCLUSION: The above founding indicated that the HUC-MSCs are able to different 
      into the functional hepatogenic-like cell by present method.
FAU - Zheng, Guan
AU  - Zheng G
AD  - Biomedical Research Center, First People's Hospital of Kunming (Affiliated 
      Calmette Hospital of Kunming Medical University), Yunnan, China.
FAU - Liu, Yang
AU  - Liu Y
AD  - Biomedical Research Center, First People's Hospital of Kunming (Affiliated 
      Calmette Hospital of Kunming Medical University), Yunnan, China.
FAU - Jing, Qian
AU  - Jing Q
AD  - Biomedical Research Center, First People's Hospital of Kunming (Affiliated 
      Calmette Hospital of Kunming Medical University), Yunnan, China.
FAU - Zhang, Lei
AU  - Zhang L
AD  - Biomedical Research Center, First People's Hospital of Kunming (Affiliated 
      Calmette Hospital of Kunming Medical University), Yunnan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biomed Mater Eng
JT  - Bio-medical materials and engineering
JID - 9104021
SB  - IM
MH  - Cell Proliferation/physiology
MH  - Cells, Cultured
MH  - Hepatocytes/*cytology/*physiology
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/*physiology
MH  - Tissue Engineering/*methods
MH  - Wharton Jelly/*cytology
OTO - NOTNLM
OT  - Human umbilical cord-derived mesenchymal stem cells (HUC-MSCs)
OT  - hepatocytes
OT  - hepatocytic differentiation
EDAT- 2014/12/30 06:00
MHDA- 2015/09/22 06:00
CRDT- 2014/12/25 06:00
PHST- 2014/12/25 06:00 [entrez]
PHST- 2014/12/30 06:00 [pubmed]
PHST- 2015/09/22 06:00 [medline]
AID - 3586271P110242K1 [pii]
AID - 10.3233/BME-141249 [doi]
PST - ppublish
SO  - Biomed Mater Eng. 2015;25(1 Suppl):145-57. doi: 10.3233/BME-141249.