PMID- 2554064
OWN - NLM
STAT- MEDLINE
DCOM- 19891124
LR  - 20190817
IS  - 0022-4731 (Print)
IS  - 0022-4731 (Linking)
VI  - 33
IP  - 4A
DP  - 1989 Oct
TI  - Expression of hydroxysteroid sulphotransferase is related to estrogen receptor 
      status in human mammary cancer.
PG  - 637-42
AB  - A positive correlation between the expression of estrogen sulphotransferase (EC 
      2.8: 2.4) and the estrogen receptor (ER) in human breast cancer tissues was 
      previously demonstrated. We have now established that a similar correlation 
      exists between the expression of hydroxysteroid sulphotransferase (EC 2.8: 2.2) 
      and ER in such tissues. Enzyme activity was present in 93% of the ER + tumor 
      cytosols (mean 59 +/- 44 (SD) pmol dehydroepiandrosterone sulphate formed per mg 
      protein per 2 h (n = 42). Activity was detected in 68% of ER - tumors and this 
      was significantly lower (mean 21 +/- 26 (SD) (n = 19), P less than 0.001) than 
      the former group. Metabolism of estradiol-17 beta (E2) and the adrenal-derived 
      estrogen 5-androstene-3 beta, 17 beta-diol (ADIOL), which is a substrate for 
      hydroxysteroid sulphotransferase but not estrogen sulphotransferase, was studied 
      in four ER + human mammary cancer cell lines (MCF-7, T47-D, MDA-MB-361 and 
      ZR-75-1) and four ER-human mammary cell lines (BT-20, MDA-MB-231, MDA-MB-330 and 
      HBL-100), employing steroid concentrations of 1 nM. At this concentration, 
      formation of ester sulphates was a major route of metabolism in the ER + cell 
      lines; E2 yielding a mean of 6.5 pmol estrogen monosulphates/mg DNA in 16 h and 
      ADIOL yielding a mean of 9.4 pmol C19-5-ene steroid monosulphates/mg DNA in 16 h. 
      In three of the four ER - cell lines, formation of sulphates from E2 occurred at 
      an eight-fold lower rate (mean 0.8 pmol estrogen sulphates/mg DNA in 16 h), 
      whereas MDA-MB-330 cells did not form estrogen sulphates. Only one of the four 
      ER- cell lines (BT-20) sulphurylated ADIOL and this was at a 12-fold lower rate 
      compared to the mean value for the ER + cel lines. Oxidation of E2 and ADIOL 
      occurred in all cell lines and was generally the major route of metabolism in the 
      ER - cells. A significant correlation between formation of estrone and 
      dehydroepiandrosterone occurred for all cell lines (r = 0.98, P less than 0.001) 
      indicating that the same 17 beta-hydroxysteroid dehydrogenase was probably 
      involved. Since ADIOL is estrogenic in a number of systems at the concentration 
      found in the blood of Western women (approximately 2 nM), the coordinated 
      expression of hydroxysteroid sulphotransferase, estrogen sulphotransferase, and 
      ER, supports the concept of a functional relationship between estrogen action via 
      ER and sulphurylation reactions.
FAU - Adams, J B
AU  - Adams JB
AD  - School of Biochemistry, University of New South Wales, Syndey, Australia.
FAU - Phillips, N S
AU  - Phillips NS
FAU - Pewnim, T
AU  - Pewnim T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Steroid Biochem
JT  - Journal of steroid biochemistry
JID - 0260125
RN  - 0 (Receptors, Estrogen)
RN  - EC 1.1.- (17-Hydroxysteroid Dehydrogenases)
RN  - EC 2.8.1.- (Sulfurtransferases)
RN  - EC 2.8.2.- (Sulfotransferases)
RN  - EC 2.8.2.2 (alcohol sulfotransferase)
RN  - EC 2.8.2.4 (estrone sulfotransferase)
SB  - IM
MH  - 17-Hydroxysteroid Dehydrogenases/metabolism
MH  - Breast Neoplasms/*metabolism
MH  - Chromatography, Thin Layer
MH  - Humans
MH  - Receptors, Estrogen/*physiology
MH  - *Sulfotransferases
MH  - Sulfurtransferases/*biosynthesis
MH  - Tumor Cells, Cultured
EDAT- 1989/10/01 00:00
MHDA- 1989/10/01 00:01
CRDT- 1989/10/01 00:00
PHST- 1989/10/01 00:00 [pubmed]
PHST- 1989/10/01 00:01 [medline]
PHST- 1989/10/01 00:00 [entrez]
AID - 10.1016/0022-4731(89)90053-8 [doi]
PST - ppublish
SO  - J Steroid Biochem. 1989 Oct;33(4A):637-42. doi: 10.1016/0022-4731(89)90053-8.