PMID- 25541196
OWN - NLM
STAT- MEDLINE
DCOM- 20150501
LR  - 20240104
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 43
IP  - 2
DP  - 2015 Jan
TI  - Cmr4 is the slicer in the RNA-targeting Cmr CRISPR complex.
PG  - 1257-67
LID - 10.1093/nar/gku1355 [doi]
AB  - Clustered regularly interspaced short palindromic repeat (CRISPR) loci and 
      CRISPR-associated (Cas) proteins form an adaptive immune system that protects 
      prokaryotes against plasmids and viruses. The Cmr complex, a type III-B effector 
      complex, uses the CRISPR RNA (crRNA) as a guide to target RNA. Here, we show that 
      the Cmr complex of Pyrococcus furiosus cleaves RNA at multiple sites that are 6 
      nt apart and are positioned relative to the 5'-end of the crRNA. We identified 
      Cmr4 as the slicer and determined its crystal structure at 2.8 A resolution. In 
      the crystal, Cmr4 forms a helical filament that most likely reflects its 
      structural organization in the Cmr complex. The putative active site is located 
      at the inner surface of the filament where the guide and substrate RNA are 
      thought to bind. The filament structure of Cmr4 accounts for multiple periodic 
      cleavage sites on the substrate. Our study provides new insights into the 
      structure and mechanism of the RNA-targeting Cmr complex.
CI  - (c) The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic 
      Acids Research.
FAU - Zhu, Xing
AU  - Zhu X
AD  - National Institute of Biological Sciences at Beijing, Beijing 102206, China 
      Department of Biochemistry and Molecular Biology, College of Life Sciences, 
      Beijing Normal University, Beijing 100875, China Key Laboratory of RNA Biology, 
      Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
FAU - Ye, Keqiong
AU  - Ye K
AD  - National Institute of Biological Sciences at Beijing, Beijing 102206, China Key 
      Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 
      Beijing 100101, China yekeqiong@nibs.ac.cn.
LA  - eng
SI  - PDB/4WNZ
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141224
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (CRISPR-Associated Proteins)
RN  - 63231-63-0 (RNA)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - CRISPR-Associated Proteins/*chemistry/*metabolism
MH  - *CRISPR-Cas Systems
MH  - Models, Molecular
MH  - Pyrococcus furiosus/enzymology
MH  - RNA/metabolism
MH  - *RNA Cleavage
MH  - Ribonucleases/*chemistry/*metabolism
PMC - PMC4333402
EDAT- 2014/12/30 06:00
MHDA- 2015/05/02 06:00
PMCR- 2014/12/24
CRDT- 2014/12/27 06:00
PHST- 2014/12/27 06:00 [entrez]
PHST- 2014/12/30 06:00 [pubmed]
PHST- 2015/05/02 06:00 [medline]
PHST- 2014/12/24 00:00 [pmc-release]
AID - gku1355 [pii]
AID - 10.1093/nar/gku1355 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2015 Jan;43(2):1257-67. doi: 10.1093/nar/gku1355. Epub 2014 
      Dec 24.