PMID- 25542136
OWN - NLM
STAT- MEDLINE
DCOM- 20160222
LR  - 20181113
IS  - 1573-4994 (Electronic)
IS  - 1053-0509 (Linking)
VI  - 25
IP  - 1
DP  - 2015 Jan
TI  - An acridine orange spore germination fluorescence microscopy versus spectral 
      paradox.
PG  - 211-6
LID - 10.1007/s10895-014-1498-5 [doi]
AB  - The metachromatic fluorophore acridine orange (AO) has demonstrated green 
      fluorescent staining of dormant Bacillus spores and orange to red staining of 
      transcriptionally active vegetative cells when used in the mid-micoMolar range. 
      Despite the microscopic observation of numerous bright orange to red fluorescent 
      vegetative cells following germination induction, no clear spectral emission 
      peaks > 590 nm have ever been reported for spectrofluorometric analysis involving 
      AO in conjunction with spore germination. This microscopy versus 
      spectrofluorometry paradox is documented in the present report and hypotheses are 
      put forth to explain the very weak spectral changes in the red region which do 
      not appear to correlate with the abundant orange-red fluorescence of nascent 
      vegetative cells seen through the fluorescence microscope.
FAU - Bruno, John G
AU  - Bruno JG
AD  - Operational Technologies Corporation, 4100 NW Loop 410, Suite 230, San Antonio, 
      TX, 78229, USA, john.bruno@otcorp.com.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20141228
PL  - Netherlands
TA  - J Fluoresc
JT  - Journal of fluorescence
JID - 9201341
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*chemistry
MH  - Bacillus thuringiensis/*cytology/*physiology
MH  - Microscopy, Fluorescence
MH  - Spectrometry, Fluorescence
MH  - Spores, Bacterial/cytology/physiology
EDAT- 2014/12/30 06:00
MHDA- 2016/02/24 06:00
CRDT- 2014/12/28 06:00
PHST- 2014/10/11 00:00 [received]
PHST- 2014/12/05 00:00 [accepted]
PHST- 2014/12/28 06:00 [entrez]
PHST- 2014/12/30 06:00 [pubmed]
PHST- 2016/02/24 06:00 [medline]
AID - 10.1007/s10895-014-1498-5 [doi]
PST - ppublish
SO  - J Fluoresc. 2015 Jan;25(1):211-6. doi: 10.1007/s10895-014-1498-5. Epub 2014 Dec 
      28.