PMID- 25543847
OWN - NLM
STAT- MEDLINE
DCOM- 20160509
LR  - 20151029
IS  - 1532-2394 (Electronic)
IS  - 0898-2104 (Linking)
VI  - 25
IP  - 4
DP  - 2015
TI  - siRNA delivery to lung-metastasized tumor by systemic injection with cationic 
      liposomes.
PG  - 279-86
LID - 10.3109/08982104.2014.992024 [doi]
AB  - CONTEXT: Cationic liposomes can efficiently deliver siRNA to the lung by 
      intravenous injection of cationic liposome/siRNA complexes (lipoplexes). 
      OBJECTIVE: The aim of this study was to examine a formulation of cationic 
      liposomes for siRNA delivery to lung metastasis of breast tumor. MATERIALS AND 
      METHODS: For the preparation of cationic liposomes, 
      1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) or dimethyldioctadecylammonium 
      bromide (DDAB) as a cationic lipid and cholesterol (Chol) or 
      1,2-dioleoyl-L-alpha-glycero-3-phosphatidylethanolamine (DOPE) as a neutral lipid 
      were used. In vitro and in vivo gene silencing effects by cationic lipoplexes 
      were evaluated after transfection into stably luciferase-expressing human breast 
      tumor MCF-7-Luc cells and after intravenous injection into mice with lung 
      MCF-7-Luc metastasis, respectively. Intracellular localization of siRNA after 
      transfection into MCF-7 cells by cationic lipoplexes and biodistribution of siRNA 
      after intravenous injection of cationic lipoplexes into the mice with lung 
      metastasis were examined by confocal and fluorescent microscopy analyses, 
      respectively. RESULTS: In in vitro transfection, DOTAP/DOPE and DDAB/DOPE 
      lipoplexes of luciferase siRNA strongly suppressed luciferase activity in 
      MCF-7-Luc cells, but DOTAP/Chol and DDAB/Chol lipoplexes did not, although 
      DOTAP/Chol and DDAB/Chol lipoplexes exhibited higher cellular uptake than 
      DOTAP/DOPE and DDAB/DOPE lipoplexes. When their cationic lipoplexes were 
      intravenously injected into mice with lung MCF-7-Luc metastasis, siRNAs were 
      mainly accumulated in the lungs; however, the reduced luciferase activities in 
      the lung-metastasized tumors were observed only by injections of DOTAP/Chol and 
      DOTAP/DOPE lipoplexes, but not by DDAB/Chol and DDAB/DOPE lipoplexes. 
      CONCLUSIONS: DOTAP-based liposomes might be useful as an in vivo siRNA delivery 
      carrier that can induce gene silencing in lung-metastasized tumors.
FAU - Hattori, Yoshiyuki
AU  - Hattori Y
AD  - a Institute of Medicinal Chemistry, Hoshi University , Shinagawa-ku , Tokyo , 
      Japan.
FAU - Nakamura, Ayako
AU  - Nakamura A
AD  - a Institute of Medicinal Chemistry, Hoshi University , Shinagawa-ku , Tokyo , 
      Japan.
FAU - Arai, Shohei
AU  - Arai S
AD  - a Institute of Medicinal Chemistry, Hoshi University , Shinagawa-ku , Tokyo , 
      Japan.
FAU - Kawano, Kumi
AU  - Kawano K
AD  - a Institute of Medicinal Chemistry, Hoshi University , Shinagawa-ku , Tokyo , 
      Japan.
FAU - Maitani, Yoshie
AU  - Maitani Y
AD  - a Institute of Medicinal Chemistry, Hoshi University , Shinagawa-ku , Tokyo , 
      Japan.
FAU - Yonemochi, Etsuo
AU  - Yonemochi E
AD  - a Institute of Medicinal Chemistry, Hoshi University , Shinagawa-ku , Tokyo , 
      Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150904
PL  - England
TA  - J Liposome Res
JT  - Journal of liposome research
JID - 9001952
RN  - 0 (Cations)
RN  - 0 (Drug Carriers)
RN  - 0 (Liposomes)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Animals
MH  - Cations/administration & dosage/chemistry
MH  - Drug Carriers/*administration & dosage/*chemistry/pharmacokinetics
MH  - Female
MH  - Gene Silencing
MH  - Humans
MH  - Injections, Intravenous
MH  - Liposomes
MH  - Lung Neoplasms/*metabolism/*secondary
MH  - MCF-7 Cells
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Particle Size
MH  - RNA, Small Interfering/*administration & dosage/genetics/*pharmacokinetics
MH  - Surface Properties
MH  - Tissue Distribution
MH  - Tumor Cells, Cultured
OTO - NOTNLM
OT  - Breast tumor
OT  - cationic liposome
OT  - gene knockdown
OT  - lung metastasis
OT  - siRNA delivery
EDAT- 2014/12/30 06:00
MHDA- 2016/05/10 06:00
CRDT- 2014/12/30 06:00
PHST- 2014/12/30 06:00 [entrez]
PHST- 2014/12/30 06:00 [pubmed]
PHST- 2016/05/10 06:00 [medline]
AID - 10.3109/08982104.2014.992024 [doi]
PST - ppublish
SO  - J Liposome Res. 2015;25(4):279-86. doi: 10.3109/08982104.2014.992024. Epub 2015 
      Sep 4.