PMID- 25545806
OWN - NLM
STAT- MEDLINE
DCOM- 20160318
LR  - 20150617
IS  - 1742-8149 (Electronic)
IS  - 1464-7273 (Linking)
VI  - 18
IP  - 2
DP  - 2015 Jun
TI  - A study of aneuploidy and DNA fragmentation in spermatozoa of three men with sex 
      chromosome mosaicism including a 45,X cell line.
PG  - 96-9
LID - 10.3109/14647273.2014.988663 [doi]
AB  - Meiotic segregation of mosaic males with a 45,X cell line has been little 
      examined. In this study, we evaluated the risk of aneuploid gametes using 
      fluorescence in situ hybridization (FISH) and DNA fragmentation in ejaculated 
      spermatozoa of three men with sex chromosome mosaicism including a 45,X cell 
      line. Triple- and dual-color FISH were performed. Sperm DNA fragmentation was 
      detected using the TUNEL assay. A significantly increased frequency of XY disomic 
      spermatozoa was observed for patients (P)1 and P2. A significant increase in 
      diploidy and autosomal aneuploidy was found in P2 and P3, respectively. The rate 
      of DNA fragmentation was not different from that observed in a control group. 
      Data from the literature are scarce (only 3 cases reported), making comparison of 
      the present data difficult, especially as the frequencies of the cell lines 
      comprising the mosaicism differed between patients. Furthermore, the proportion 
      of the different cell lines can differ from one tissue to another in the same 
      patient. Whether the relative levels of the several cell lines present in the 
      mosaicism can influence the rate of aneuploid spermatozoa remains unknown.
FAU - Nguyen, Minh Huong
AU  - Nguyen MH
AD  - Laboratoire d'Histologie, Embryologie et Cytogenetique, Faculte de Medecine et 
      des Sciences de la Sante, Universite de Bretagne Occidentale , Brest , France.
FAU - Morel, Frederic
AU  - Morel F
FAU - Bujan, Louis
AU  - Bujan L
FAU - May-Panloup, Pascale
AU  - May-Panloup P
FAU - De Braekeleer, Marc
AU  - De Braekeleer M
FAU - Perrin, Aurore
AU  - Perrin A
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20141229
PL  - England
TA  - Hum Fertil (Camb)
JT  - Human fertility (Cambridge, England)
JID - 100888143
SB  - IM
MH  - *Aneuploidy
MH  - Cell Line
MH  - *DNA Fragmentation
MH  - Humans
MH  - Male
MH  - *Mosaicism
MH  - Sex Chromosome Disorders/*pathology
MH  - Sex Chromosomes
MH  - Spermatozoa/*pathology
OTO - NOTNLM
OT  - DNA fragmentation
OT  - Sex chromosome mosaicism
OT  - aneuploidy
OT  - meiotic segregation
EDAT- 2014/12/30 06:00
MHDA- 2016/03/19 06:00
CRDT- 2014/12/30 06:00
PHST- 2014/12/30 06:00 [entrez]
PHST- 2014/12/30 06:00 [pubmed]
PHST- 2016/03/19 06:00 [medline]
AID - 10.3109/14647273.2014.988663 [doi]
PST - ppublish
SO  - Hum Fertil (Camb). 2015 Jun;18(2):96-9. doi: 10.3109/14647273.2014.988663. Epub 
      2014 Dec 29.