PMID- 25547245
OWN - NLM
STAT- MEDLINE
DCOM- 20160613
LR  - 20211119
IS  - 1860-1499 (Electronic)
IS  - 1860-1499 (Linking)
VI  - 48
IP  - 3
DP  - 2015 Sep
TI  - Expression of interleukin-34 and colony stimulating factor-1 in the stimulated 
      periodontal ligament cells with tumor necrosis factor-alpha.
PG  - 169-76
LID - 10.1007/s00795-014-0094-8 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) directly and indirectly plays a crucial role in 
      osteoclastogenesis. However, the indirect effects of TNF-alpha on colony-stimulating 
      factor-1 receptor (CSF-1R)-mediated osteoclastogenesis achieved via periodontal 
      ligament (PDL) cells are not fully understood. We herein examined the potency of 
      osteoclast differentiation and maturation induced by fivefold supernatants in the 
      stimulated human PDL cells with a physiologically high concentration (10 ng/mL) 
      of recombinant TNF-alpha to human peripheral blood monocytes/macrophages in the 
      simultaneous presence of the receptor activator of nuclear factor kappa-B ligand. 
      The number of tartrate-resistant acid phosphatase-positive cells with multiple 
      nuclei, but not those with a single nucleus, was decreased by approximately 50% 
      by neutralization with rabbit IgG against either interleukin-34 (IL-34) or CSF-1. 
      Small and large amounts of IL34 and CSF1 transcripts were measured in the 
      stimulated PDL cells using real-time polymerase chain reaction. The corresponding 
      amounts of proteins to IL34 and CSF1 transcripts were observed in the stimulated 
      PDL cells on immunohistochemical staining or Western blotting. Moreover, 0.13 
      ng/mL of IL-34 and 5.0 ng/mL of CSF-1 were measured in the supernatants of the 
      stimulated PDL cells using an enzyme-linked immunosorbent assay. IL-34 derived 
      from the stimulated PDL cells with TNF-alpha appeared to synergistically function 
      with CSF-1 in the CSF-1R-mediated maturation of osteoclastogenesis.
FAU - Kawabe, Mutsuki
AU  - Kawabe M
AD  - Department of Oral and Maxillofacial Surgery, Hyogo College of Medicine, 1-1 
      Mukogawa-cho, Nishinomiya, 663-8501, Japan. muttsu@hyo-med.ac.jp.
FAU - Ohyama, Hideki
AU  - Ohyama H
AD  - Department of Pathology, Hyogo College of Medicine, Nishinomiya, 663-8501, Japan.
FAU - Kato-Kogoe, Nahoko
AU  - Kato-Kogoe N
AD  - Department of Pathology, Hyogo College of Medicine, Nishinomiya, 663-8501, Japan.
FAU - Yamada, Naoko
AU  - Yamada N
AD  - Department of Pathology, Hyogo College of Medicine, Nishinomiya, 663-8501, Japan.
FAU - Yamanegi, Koji
AU  - Yamanegi K
AD  - Department of Pathology, Hyogo College of Medicine, Nishinomiya, 663-8501, Japan.
FAU - Nishiura, Hiroshi
AU  - Nishiura H
AD  - Department of Pathology, Hyogo College of Medicine, Nishinomiya, 663-8501, Japan.
FAU - Hirano, Hirotugu
AU  - Hirano H
AD  - Department of Laboratory Medicine, Toneyama National Hospital, Osaka, 560-8552, 
      Japan.
FAU - Kishimoto, Hiromitsu
AU  - Kishimoto H
AD  - Department of Oral and Maxillofacial Surgery, Hyogo College of Medicine, 1-1 
      Mukogawa-cho, Nishinomiya, 663-8501, Japan.
FAU - Nakasho, Keiji
AU  - Nakasho K
AD  - Department of Pathology, Hyogo College of Medicine, Nishinomiya, 663-8501, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141230
PL  - Japan
TA  - Med Mol Morphol
JT  - Medical molecular morphology
JID - 101239023
RN  - 0 (IL34 protein, human)
RN  - 0 (Interleukins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - *Cell Differentiation
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukins/analysis/genetics/*metabolism
MH  - Macrophage Colony-Stimulating Factor/analysis/genetics/*metabolism
MH  - Periodontal Ligament/*cytology/drug effects/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Stimulation, Chemical
MH  - Tumor Necrosis Factor-alpha/*physiology
OTO - NOTNLM
OT  - Colony-stimulating factor-1
OT  - Interleukin-34
OT  - Osteoclastogenesis
OT  - Periodontal ligament cells
OT  - Tumor necrosis factor-alpha
EDAT- 2014/12/31 06:00
MHDA- 2016/06/14 06:00
CRDT- 2014/12/31 06:00
PHST- 2014/11/14 00:00 [received]
PHST- 2014/12/17 00:00 [accepted]
PHST- 2014/12/31 06:00 [entrez]
PHST- 2014/12/31 06:00 [pubmed]
PHST- 2016/06/14 06:00 [medline]
AID - 10.1007/s00795-014-0094-8 [pii]
AID - 10.1007/s00795-014-0094-8 [doi]
PST - ppublish
SO  - Med Mol Morphol. 2015 Sep;48(3):169-76. doi: 10.1007/s00795-014-0094-8. Epub 2014 
      Dec 30.