PMID- 25547701
OWN - NLM
STAT- MEDLINE
DCOM- 20150908
LR  - 20181202
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 94
IP  - 38
DP  - 2014 Oct 21
TI  - [Effects of insulin on pigment epithelium-derived factor expression in adipocytes 
      of type 2 diabetic mellitus in rats].
PG  - 2992-5
AB  - OBJECTIVE: To explore the effects of insulin therapy on the expression of pigment 
      epithelium-derived factor (PEDF) in adipocytes of type 2 diabetic mellitus (T2DM) 
      in rats. METHODS: A total of 22 newly diagnosed type 2 diabetics received a 
      2-week intensive insulin therapy. The levels of fasting plasma glucose (FPG), 
      serum triglyceride and PEDF were measured before and after therapy. T2DM was 
      induced by a high-fat diet and a low-dose streptozotocin (STZ). The 
      Spraque-Dawley rats were divided randomly into diabetic, insulin treatment and 
      gliclazide treatment groups. Another group with a chow diet was designated as 
      normal controls. Differentiated 3T3-L1 adipocytes were then incubated with tumor 
      necrosis factor-alpha (TNF-alpha) and (or) insulin for 24 h. Quantitative reverse 
      transcription-polymerase chain reaction (qRT-PCR) and Western blot were used to 
      detect the expression of PEDF in adipose tissue or adipocytes. The PEDF levels in 
      both sera and cell supernatant were measured by enzyme-linked immunosorbent assay 
      (ELISA). Glucose uptake was detected after treatment of PEDF or anti-PEDF 
      antibody simultaneously together with insulin in mature 3T3-L1 adipocytes. 
      RESULTS: Insulin therapy decreased the serum levels of FPG and triglyceride of 
      T2DM patients ((12.9 +/- 2.8) vs (5.9 +/- 1.4) mmol/L, (3.1 +/- 1.8) vs (1.7 +/- 0.8) 
      mmol/L, P < 0.05) while the serum level of PEDF decreased significantly after 
      therapy ((22.85 +/- 5.73) vs (18.38 +/- 5.28) microg/L, P < 0.05). Consistently the serum 
      level of PEDF of diabetic rats was remarkably higher than that of normal controls 
      and insulin-treated group ((28.6 +/- 0.5) vs (25.4 +/- 0.6) and (25.3 +/- 0.6) microg/L, P 
      < 0.05). And the elevated levels of PEDF, TNF-alpha mRNA and protein in adipose 
      tissue (P < 0.05) could be reduced by insulin treatment (P < 0.05). However, no 
      obvious change was detected in gliclazide treatment group. Further evidences 
      suggested that TNF-alpha could induce more secretion and expression of PEDF in 3T3-L1 
      adipocyte while this effect became ameliorated by insulin treatment. Furthermore, 
      decreased capacity of glucose uptake by PEDF might be reversed by anti-PEDF 
      antibody in 3T3-L1 adipocytes (P < 0.05). CONCLUSIONS: Insulin can down-regulate 
      the expression of PEDF in adipocytes of T2DM and improve the glucose uptake of 
      adipocytes. It may be one of the mechanisms through which insulin therapy 
      improves peripheral insulin resistance.
FAU - Xu, Fen
AU  - Xu F
AD  - Department of Endocrinology, Third Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou 510630, China.
FAU - Lin, Beisi
AU  - Lin B
FAU - Zhou, Yinli
AU  - Zhou Y
FAU - Deng, Hongrong
AU  - Deng H
FAU - Bi, Yan
AU  - Bi Y
FAU - Liang, Hua
AU  - Liang H
AD  - Email: letitia.yw@163.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Eye Proteins)
RN  - 0 (Insulin)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Serpins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (pigment epithelium-derived factor)
SB  - IM
MH  - 3T3-L1 Cells
MH  - *Adipocytes
MH  - Adipose Tissue
MH  - Animals
MH  - Cell Differentiation
MH  - Diabetes Mellitus, Experimental
MH  - *Diabetes Mellitus, Type 2
MH  - Diet, High-Fat
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Eye Proteins
MH  - Insulin
MH  - Mice
MH  - Nerve Growth Factors
MH  - Rats
MH  - Serpins
MH  - Tumor Necrosis Factor-alpha
EDAT- 2014/12/31 06:00
MHDA- 2015/09/09 06:00
CRDT- 2014/12/31 06:00
PHST- 2014/12/31 06:00 [entrez]
PHST- 2014/12/31 06:00 [pubmed]
PHST- 2015/09/09 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2014 Oct 21;94(38):2992-5.