PMID- 25547710
OWN - NLM
STAT- MEDLINE
DCOM- 20150908
LR  - 20181202
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 94
IP  - 38
DP  - 2014 Oct 21
TI  - [Effects of hydrogen-rich saline on endoplasmic reticulum stress during 
      myocardial ischemia-reperfusion in rats].
PG  - 3024-8
AB  - OBJECTIVE: To explore the effects of hydrogen-rich saline on endoplasmic 
      reticulum stress during myocardial ischemia-reperfusion (I/R) in rats. METHODS: A 
      total of 150 healthy male Sprague-Dawley rats were selected and then randomly 
      divided into 5 groups of normal control (I), sham operation (II), myocardial 
      ischemia-reperfusion (III), hydrogen-rich saline (IV) and normal saline (V) (n = 
      30 each). Group I had no treatment. In group II, anterior descending branch was 
      merely exposed but not ligated. Myocardial I/R was induced by an occlusion of 
      anterior descending branch of left coronary artery for 30 min followed by 12 h 
      and 24 h of reperfusion with bimbaum. Hydrogen-rich saline 1 ml/100 g was 
      injected intraperitoneally 5 min before reperfusion in group IV. Normal saline 1 
      ml/100 g was injected intraperitoneally 5 min before reperfusion in group V. The 
      rats were sacrificed at 12 h and 24 h of reperfusion and heart tissues harvested. 
      The pathological changes of myocardial tissue were detected by hematoxylin & 
      eosin staining. The apoptotic cardiomyocytes of myocardial tissue were tested by 
      terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). 
      And the expressions of GRP78, Caspase-12, Bcl-2 and Bax in myocardial tissue were 
      detected by Western blot. RESULTS: Compared with groups I and II, the numbers of 
      apoptotic cardiomyocytes, the expression of GRP78, Caspase-12 and Bax in 
      myocardial tissue significantly increased and the expression of Bcl-2 in 
      myocardial tissue significantly decreased in groups III, IV and V. Compared with 
      group III and V, the numbers of apoptotic cardiomyocytes, the expression of 
      GRP78, Caspase-12 and Bax in myocardial tissue significantly decreased while the 
      expression of Bcl-2 in myocardial tissue significantly increased in group IV. 
      CONCLUSIONS: Hydrogen-rich saline may decrease cell apoptosis and attenuate 
      myocardial reperfusion injury through inhibiting endoplasmic reticulum stress. 
      The mechanism may be associated with decreasing the expression of GRP78, 
      Caspase-12 and Bax and increasing the expression of Bcl-2 in myocardial tissue.
FAU - Zhao, Yue
AU  - Zhao Y
AD  - Department of Cardiology, Daqing Oil Field General Hospital, Daqing 163001, 
      China.
FAU - Tang, Yulong
AU  - Tang Y
AD  - Department of Cardiology, Daqing Oil Field General Hospital, Daqing 163001, 
      China.
FAU - Suo, Chuantao
AU  - Suo C
AD  - Department of Cardiology, Daqing Oil Field General Hospital, Daqing 163001, 
      China.
FAU - Liu, Dayi
AU  - Liu D
AD  - Department of Cardiology, Daqing Oil Field General Hospital, Daqing 163001, 
      China.
FAU - Li, Shiyang
AU  - Li S
AD  - Department of Cardiology, Daqing Oil Field General Hospital, Daqing 163001, 
      China.
FAU - Li, Hui
AU  - Li H
AD  - Department of Cardiology, Daqing Oil Field General Hospital, Daqing 163001, 
      China. Email: 791685879@qq.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - EC 3.4.22.- (Casp12 protein, rat)
RN  - EC 3.4.22.- (Caspase 12)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Caspase 12
MH  - Endoplasmic Reticulum Stress
MH  - Hydrogen
MH  - *Ischemia
MH  - Male
MH  - *Myocardial Reperfusion Injury
MH  - Proto-Oncogene Proteins c-bcl-2
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sodium Chloride
MH  - bcl-2-Associated X Protein
EDAT- 2014/12/31 06:00
MHDA- 2015/09/09 06:00
CRDT- 2014/12/31 06:00
PHST- 2014/12/31 06:00 [entrez]
PHST- 2014/12/31 06:00 [pubmed]
PHST- 2015/09/09 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2014 Oct 21;94(38):3024-8.