PMID- 25547899
OWN - NLM
STAT- MEDLINE
DCOM- 20151207
LR  - 20171116
IS  - 1618-0631 (Electronic)
IS  - 0344-0338 (Linking)
VI  - 211
IP  - 3
DP  - 2015 Mar
TI  - High expression of vacuolar protein sorting 4B (VPS4B) is associated with 
      accelerated cell proliferation and poor prognosis in human hepatocellular 
      carcinoma.
PG  - 240-7
LID - S0344-0338(14)00344-6 [pii]
LID - 10.1016/j.prp.2014.11.013 [doi]
AB  - Vacuolar protein sorting 4B (VPS4B) is a member of ATPase family proteins that 
      have been shown to play important roles in the formation of MVBs, virus budding 
      and abscission of cytokinesis. In this study, we investigated the prognostic role 
      of VPS4B in human hepatocellular carcinoma (HCC) and its effect on the growth of 
      HCC cells. Western blot and immunohistochemistrical analyses revealed that VPS4B 
      was significantly upregulated in 98 HCC tissues, compared with adjacent 
      nontumorous samples. Meanwhile, clinicopathological variables and univariate and 
      multivariate survival analyses showed that high VPS4B expression was correlated 
      with multiple clinicopathological factors, including AJCC stage, microvascular 
      invasion, Ki-67 and a poor prognosis. More importantly, univariate and 
      multivariate survival analyses demonstrated that VPS4B served as an independent 
      prognostic factor for survival in HCC patients. Furthermore, we found that VPS4B 
      was lowly expressed in serum-starved Huh7 and HepG2 HCC cells, and was 
      progressively increased after serum-refeeding. To study whether VPS4B could 
      regulate the proliferation of HCC cells, VPS4B was knocked down in both Huh7 and 
      HepG2 cells through the transfection of VPS4B-siRNA oligos. Flow cytometry and 
      CCK-8 assay results indicated that interference of VPS4B led to cell cycle arrest 
      and reduced cell proliferation of HCC cells. Taken together, our results implied 
      that VPS4B could be a candidate prognostic biomarker as well as a potential 
      therapeutical target of HCC.
CI  - Copyright (c) 2014 Elsevier GmbH. All rights reserved.
FAU - Jiang, Dawei
AU  - Jiang D
AD  - Department of Digestion, Affiliated Hospital of Nantong University, Medical 
      College of Nantong University, Nantong, Jiangsu 226001, People's Republic of 
      China.
FAU - Hu, Baoying
AU  - Hu B
AD  - Medical College, Nantong University, Nantong, Jiangsu 226001, People's Republic 
      of China.
FAU - Wei, Lixian
AU  - Wei L
AD  - Department of Digestion, Affiliated Hospital of Nantong University, Medical 
      College of Nantong University, Nantong, Jiangsu 226001, People's Republic of 
      China.
FAU - Xiong, Yicheng
AU  - Xiong Y
AD  - Department of General Surgery, Affiliated Hospital of Nantong University, Medical 
      College of Nantong University, Nantong, Jiangsu 226001, People's Republic of 
      China.
FAU - Wang, Gang
AU  - Wang G
AD  - Department of General Surgery, Affiliated Hospital of Nantong University, Medical 
      College of Nantong University, Nantong, Jiangsu 226001, People's Republic of 
      China.
FAU - Ni, Tingting
AU  - Ni T
AD  - Department of Oncology, Affiliated Hospital of Nantong University, Medical 
      College of Nantong University, Nantong, Jiangsu 226001, People's Republic of 
      China.
FAU - Zong, Chunyan
AU  - Zong C
AD  - Department of Digestion, Affiliated Hospital of Nantong University, Medical 
      College of Nantong University, Nantong, Jiangsu 226001, People's Republic of 
      China.
FAU - Ni, Runzhou
AU  - Ni R
AD  - Department of Digestion, Affiliated Hospital of Nantong University, Medical 
      College of Nantong University, Nantong, Jiangsu 226001, People's Republic of 
      China.
FAU - Lu, Cuihua
AU  - Lu C
AD  - Department of Digestion, Affiliated Hospital of Nantong University, Medical 
      College of Nantong University, Nantong, Jiangsu 226001, People's Republic of 
      China. Electronic address: luch2216141201@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141129
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.4.- (ATPases Associated with Diverse Cellular Activities)
RN  - EC 3.6.4.6 (VPS4B protein, human)
SB  - IM
MH  - ATPases Associated with Diverse Cellular Activities
MH  - Adenosine Triphosphatases/*metabolism
MH  - Adult
MH  - Aged
MH  - Carcinoma, Hepatocellular/*metabolism/mortality/pathology
MH  - Cell Proliferation/*physiology
MH  - Endosomal Sorting Complexes Required for Transport/*metabolism
MH  - Female
MH  - Humans
MH  - Liver Neoplasms/*metabolism/mortality/pathology
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - Survival Analysis
MH  - *Up-Regulation
MH  - Young Adult
OTO - NOTNLM
OT  - Cell proliferation
OT  - Hepatocellular carcinoma
OT  - Ki-67
OT  - Prognosis
OT  - Vacuolar protein sorting 4B (VPS4B)
EDAT- 2014/12/31 06:00
MHDA- 2015/12/15 06:00
CRDT- 2014/12/31 06:00
PHST- 2014/08/02 00:00 [received]
PHST- 2014/10/26 00:00 [revised]
PHST- 2014/11/21 00:00 [accepted]
PHST- 2014/12/31 06:00 [entrez]
PHST- 2014/12/31 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - S0344-0338(14)00344-6 [pii]
AID - 10.1016/j.prp.2014.11.013 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2015 Mar;211(3):240-7. doi: 10.1016/j.prp.2014.11.013. Epub 
      2014 Nov 29.