PMID- 25549249
OWN - NLM
STAT- MEDLINE
DCOM- 20150828
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 12
DP  - 2014
TI  - Identification of protein network alterations upon retinal ischemia-reperfusion 
      injury by quantitative proteomics using a Rattus norvegicus model.
PG  - e116453
LID - 10.1371/journal.pone.0116453 [doi]
LID - e116453
AB  - Retinal ischemia is a common feature associated with several ocular diseases, 
      including diabetic retinopathy. In this study, we investigated the effect of a 
      retinal ischemia and reperfusion (I/R) injury on protein levels via a 
      quantitative shotgun strategy using stable isotope dimethyl labeling combined 
      with LC-MS/MS analysis. Based on the relative quantitation data of 1088 proteins, 
      234 proteins showed a greater than 1.5-fold change following I/R injury, 194 of 
      which were up-regulated and 40 were down-regulated. Gene ontology analysis 
      revealed that after I/R injury, there was an increase in the metabolic-process 
      related proteins but a decline in cell communication, system process and 
      transport-related proteins. A ribosome protein network and a secreted protein 
      network consisting of many protease inhibitors were identified among the 
      up-regulated proteins, despite a suppression of the mammalian target of rapamycin 
      (mTOR) pathway following the I/R injury. A synaptic-related protein network was 
      found to be significantly down-regulated, implicating a functional reduction of 
      neurons following a retinal I/R injury. Our results provide new systems-biology 
      clues for the study of retinal ischemia.
FAU - Tian, Han
AU  - Tian H
AD  - College of Life Sciences, Wuhan University, Wuhan, China.
FAU - Wang, Leilei
AU  - Wang L
AD  - College of Life Sciences, Wuhan University, Wuhan, China.
FAU - Cai, Ruiqi
AU  - Cai R
AD  - College of Life Sciences, Wuhan University, Wuhan, China.
FAU - Zheng, Ling
AU  - Zheng L
AD  - College of Life Sciences, Wuhan University, Wuhan, China.
FAU - Guo, Lin
AU  - Guo L
AD  - College of Life Sciences, Wuhan University, Wuhan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141230
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chromatography, Liquid/methods
MH  - Disease Models, Animal
MH  - Gene Expression Regulation
MH  - *Gene Regulatory Networks
MH  - Humans
MH  - Male
MH  - Proteomics/*methods
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/*metabolism
MH  - Retinal Diseases/*metabolism
MH  - Signal Transduction
MH  - Systems Biology/*methods
MH  - Tandem Mass Spectrometry/methods
PMC - PMC4280217
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/12/31 06:00
MHDA- 2015/09/01 06:00
PMCR- 2014/12/30
CRDT- 2014/12/31 06:00
PHST- 2014/08/26 00:00 [received]
PHST- 2014/12/08 00:00 [accepted]
PHST- 2014/12/31 06:00 [entrez]
PHST- 2014/12/31 06:00 [pubmed]
PHST- 2015/09/01 06:00 [medline]
PHST- 2014/12/30 00:00 [pmc-release]
AID - PONE-D-14-38274 [pii]
AID - 10.1371/journal.pone.0116453 [doi]
PST - epublish
SO  - PLoS One. 2014 Dec 30;9(12):e116453. doi: 10.1371/journal.pone.0116453. 
      eCollection 2014.