PMID- 25550697
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141231
LR  - 20200929
IS  - 1598-2629 (Print)
IS  - 2092-6685 (Electronic)
IS  - 1598-2629 (Linking)
VI  - 14
IP  - 6
DP  - 2014 Dec
TI  - Characterization of Proinflammatory Responses and Innate Signaling Activation in 
      Macrophages Infected with Mycobacterium scrofulaceum.
PG  - 307-20
LID - 10.4110/in.2014.14.6.307 [doi]
AB  - Mycobacterium scrofulaceum is an environmental and slow-growing atypical 
      mycobacterium. Emerging evidence suggests that M. scrofulaceum infection is 
      associated with cervical lymphadenitis in children and pulmonary or systemic 
      infections in immunocompromised adults. However, the nature of host innate immune 
      responses to M. scrofulaceum remains unclear. In this study, we examined the 
      innate immune responses in murine bone marrow-derived macrophages (BMDMs) 
      infected with different M. scrofulaceum strains including ATCC type strains and 
      two clinically isolated strains (rough and smooth types). All three strains 
      resulted in the production of proinflammatory cytokines in BMDMs mediated through 
      toll-like receptor-2 and the adaptor MyD88. Activation of MAPKs (extracellular 
      signal-regulated kinase 1/2, and p38, and c-Jun N-terminal kinase) and nuclear 
      receptor (NF)-kappaB together with intracellular reactive oxygen species generation 
      were required for the expression of proinflammatory cytokines in BMDMs. In 
      addition, the rough morphotypes of M. scrofulaceum clinical strains induced 
      higher levels of proinflammatory cytokines, MAPK and NF-kappaB activation, and ROS 
      production than other strains. When mice were infected with different M. 
      scrofulaceum strains, those infected with the rough strain showed the greatest 
      hepatosplenomegaly, granulomatous lesions, and immune cell infiltration in the 
      lungs. Notably, the bacterial load was higher in mice infected with rough 
      colonies than in mice infected with ATCC or smooth strains. Collectively, these 
      data indicate that rough M. scrofulaceum induces higher inflammatory responses 
      and virulence than ATCC or smooth strains.
FAU - Kim, Ki-Hye
AU  - Kim KH
AD  - Center of Inflammation, Infection & Immunity, Institute for Biomedical Sciences, 
      Georgia State University, Atlanta, GA, USA.
FAU - Kim, Tae-Sung
AU  - Kim TS
AD  - Department of Microbiology, Chungnam National University School of Medicine, 
      Daejeon 301-747, Korea. ; Infection Signaling Network Research Center, Chungnam 
      National University School of Medicine, Daejeon 301-747, Korea.
FAU - Lee, Joy G
AU  - Lee JG
AD  - Department of Microbiology, Chungnam National University School of Medicine, 
      Daejeon 301-747, Korea. ; Infection Signaling Network Research Center, Chungnam 
      National University School of Medicine, Daejeon 301-747, Korea.
FAU - Park, Jeong-Kyu
AU  - Park JK
AD  - Department of Microbiology, Chungnam National University School of Medicine, 
      Daejeon 301-747, Korea. ; Infection Signaling Network Research Center, Chungnam 
      National University School of Medicine, Daejeon 301-747, Korea.
FAU - Yang, Miso
AU  - Yang M
AD  - Department of Microbiology, Chungnam National University School of Medicine, 
      Daejeon 301-747, Korea. ; Infection Signaling Network Research Center, Chungnam 
      National University School of Medicine, Daejeon 301-747, Korea.
FAU - Kim, Jin-Man
AU  - Kim JM
AD  - Infection Signaling Network Research Center, Chungnam National University School 
      of Medicine, Daejeon 301-747, Korea. ; Department of Pathology, Chungnam National 
      University School of Medicine, Daejeon 301-747, Korea.
FAU - Jo, Eun-Kyeong
AU  - Jo EK
AD  - Department of Microbiology, Chungnam National University School of Medicine, 
      Daejeon 301-747, Korea. ; Infection Signaling Network Research Center, Chungnam 
      National University School of Medicine, Daejeon 301-747, Korea.
FAU - Yuk, Jae-Min
AU  - Yuk JM
AD  - Department of Infection Biology, Chungnam National University School of Medicine, 
      Daejeon 301-747, Korea. ; Infection Signaling Network Research Center, Chungnam 
      National University School of Medicine, Daejeon 301-747, Korea.
LA  - eng
PT  - Journal Article
DEP - 20141222
PL  - Korea (South)
TA  - Immune Netw
JT  - Immune network
JID - 101137270
PMC - PMC4275388
OTO - NOTNLM
OT  - MAPKs
OT  - MyD88
OT  - Mycobacterium scrofulaceum
OT  - Nuclear receptor-kappaB
OT  - Proinflammatory cytokines
OT  - TLR
COIS- The authors have no financial conflict of interest.
EDAT- 2015/01/01 06:00
MHDA- 2015/01/01 06:01
PMCR- 2014/12/01
CRDT- 2015/01/01 06:00
PHST- 2014/10/03 00:00 [received]
PHST- 2014/11/12 00:00 [revised]
PHST- 2014/11/20 00:00 [accepted]
PHST- 2015/01/01 06:00 [entrez]
PHST- 2015/01/01 06:00 [pubmed]
PHST- 2015/01/01 06:01 [medline]
PHST- 2014/12/01 00:00 [pmc-release]
AID - 10.4110/in.2014.14.6.307 [doi]
PST - ppublish
SO  - Immune Netw. 2014 Dec;14(6):307-20. doi: 10.4110/in.2014.14.6.307. Epub 2014 Dec 
      22.