PMID- 25551469
OWN - NLM
STAT- MEDLINE
DCOM- 20151005
LR  - 20181113
IS  - 1471-230X (Electronic)
IS  - 1471-230X (Linking)
VI  - 14
DP  - 2014 Dec 31
TI  - Anti-Outer membrane protein C and anti-glycoprotein 2 antibodies in inflammatory 
      bowel disease and their association with complicated forms of Crohn's disease.
PG  - 190
LID - 10.1186/s12876-014-0190-1 [doi]
LID - 190
AB  - BACKGROUND: Precise diagnostics of inflammatory bowel disease (IBD) and 
      identification of potentially more aggressive phenotypes of Crohn's disease (CD) 
      is urgently needed. The aim of our prospective study was to assess the 
      relationship between serum anti-OmpC IgA (Outer membrane protein C), anti-GP2 
      (anti-glycoprotein 2) IgG and anti-GP2 IgA antibodies with IBD and their 
      association with complicated forms of CD. METHODS: The study included 86 patients 
      with CD, 25 patients with UC and 45 controls, blood donors. In CD group, 24/86 
      (28%) had B1 phenotype, 20/86 (23%) B2, 13/86 (15%) B3 and 29/86 (34%) B2 + B3. 
      L1 involvement was present in 13/86 (15%), L2 in 13/86 (15%), L3 in 60/86 (70%). 
      Serum anti-OmpC IgA, anti-GP2 IgG and IgA antibodies were investigated by means 
      of ELISA. The data obtained were tested statistically by means of descriptive 
      statistics, non-paired t-test, Mann-Whitney rank sum test, Spearman rank order 
      correlation and Pearson product moment correlation using SigmaStat software. 
      RESULTS: Anti-OmpC IgA were noted to be significantly higher in CD (median 32.6, 
      inter-quartile range (IQR) 18.9-60.7) compared to the controls (median 18.3, IQR 
      11.1-23.1), p < 0.001. Anti-GP2 IgG were significantly higher in CD (median 13.9, 
      IQR 8.6-25.6) compared to the controls (median 8.0, IQR 4.7-10.8), p < 0.001. 
      Anti-GP2 IgA were significantly higher in CD (median 20.1, IQR 9.1-40.4) compared 
      to the controls (median 9.8, IQR 5.6-16.9), p < 0.001. Significant difference was 
      found in anti-OmpC IgA between UC (median 26.2, IQR 20.2-36.4) and the controls 
      (median 18.3, IQR 11.1-23.1), p < 0.001. In CD anti-OmpC IgA were significantly 
      higher in B2 compared to B1: p = 0.041 and in B2 + B3 compared to B1: p = 0.036. 
      Anti-GP2 IgA were significantly higher in B2 + B3 compared to B1: p = 0.009 and 
      in B3 compared to B1: p = 0.029. In CD there was a significant difference in 
      anti-OmpC IgA between patients with surgery and without surgery, p = 0.005. 
      CONCLUSIONS: We have confirmed association between anti-OmpC IgA and IBD (CD and 
      UC) and an association between anti-GP2 (IgG and IgA) and CD. Patients with 
      complicated forms of CD have significantly higher levels of anti-OmpC IgA and 
      anti-GP2 IgA.
FAU - Kohoutova, Darina
AU  - Kohoutova D
FAU - Drahosova, Marcela
AU  - Drahosova M
FAU - Moravkova, Paula
AU  - Moravkova P
FAU - Rejchrt, Stanislav
AU  - Rejchrt S
FAU - Bures, Jan
AU  - Bures J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141231
PL  - England
TA  - BMC Gastroenterol
JT  - BMC gastroenterology
JID - 100968547
RN  - 0 (Glycoproteins)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (OmpC protein)
RN  - 0 (Porins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Crohn Disease/*diagnosis/immunology
MH  - Female
MH  - Glycoproteins/*immunology
MH  - Humans
MH  - Immunoglobulin A/*blood
MH  - Immunoglobulin G/*blood
MH  - Inflammatory Bowel Diseases/*diagnosis/immunology
MH  - Male
MH  - Middle Aged
MH  - Porins/*immunology
MH  - Prospective Studies
MH  - Young Adult
PMC - PMC4296537
EDAT- 2015/01/01 06:00
MHDA- 2015/10/06 06:00
PMCR- 2014/12/31
CRDT- 2015/01/01 06:00
PHST- 2014/01/29 00:00 [received]
PHST- 2014/10/17 00:00 [accepted]
PHST- 2015/01/01 06:00 [entrez]
PHST- 2015/01/01 06:00 [pubmed]
PHST- 2015/10/06 06:00 [medline]
PHST- 2014/12/31 00:00 [pmc-release]
AID - s12876-014-0190-1 [pii]
AID - 190 [pii]
AID - 10.1186/s12876-014-0190-1 [doi]
PST - epublish
SO  - BMC Gastroenterol. 2014 Dec 31;14:190. doi: 10.1186/s12876-014-0190-1.