PMID- 25552276
OWN - NLM
STAT- MEDLINE
DCOM- 20151125
LR  - 20191210
IS  - 1943-2631 (Electronic)
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 199
IP  - 3
DP  - 2015 Mar
TI  - A HIF-independent mediator of transcriptional responses to oxygen deprivation in 
      Caenorhabditis elegans.
PG  - 739-48
LID - 10.1534/genetics.114.173989 [doi]
AB  - The adaptive response to hypoxia is accompanied by widespread transcriptional 
      changes that allow for prolonged survival in low oxygen. Many of these changes 
      are directly regulated by the conserved hypoxia-inducible factor-1 (HIF-1) 
      complex; however, even in its absence, many oxygen-sensitive transcripts in 
      Caenorhabditis elegans are appropriately regulated in hypoxia. To identify 
      mediators of these non-HIF-dependent responses, we established a hif-1 mutant 
      reporter line that expresses GFP in hypoxia or when worms are treated with the 
      hypoxia mimetic cobalt chloride (CoCl2). The reporter is selective and HIF 
      independent, in that it remains insensitive to a number of cellular stresses, but 
      is unaffected by mutation of the prolyl hydroxylase egl-9, suggesting that the 
      regulators of this response pathway are different from those controlling the HIF 
      pathway. We used the HIF-independent reporter to screen a transcription factor 
      RNA interference (RNAi) library and identified genes that are required for 
      hypoxia-sensitive and CoCl2-induced GFP expression. We identified the zinc finger 
      protein BLMP-1 as a mediator of the HIF-independent response. We show that 
      mutation of blmp-1 renders animals sensitive to hypoxic exposure and that blmp-1 
      is required for appropriate hypoxic-induced expression of HIF-independent 
      transcripts. Further, we demonstrate that BLMP-1 is necessary for an increase of 
      hypoxia-dependent histone acetylation within the promoter of a non-HIF-dependent 
      hypoxia response gene.
CI  - Copyright (c) 2015 by the Genetics Society of America.
FAU - Padmanabha, Divya
AU  - Padmanabha D
AD  - Department of Biochemistry and Molecular Biology, Virginia Commonwealth 
      University School of Medicine, Richmond, Virginia 23298 Massey Cancer Center, 
      Virginia Commonwealth University School of Medicine, Richmond, Virginia 23298.
FAU - Padilla, Pamela A
AU  - Padilla PA
AD  - Department of Biological Sciences, University of North Texas, Denton, Texas 
      76203.
FAU - You, Young-Jai
AU  - You YJ
AD  - Department of Biochemistry and Molecular Biology, Virginia Commonwealth 
      University School of Medicine, Richmond, Virginia 23298.
FAU - Baker, Keith D
AU  - Baker KD
AUID- ORCID: 0000-0002-6755-1697
AD  - Department of Biochemistry and Molecular Biology, Virginia Commonwealth 
      University School of Medicine, Richmond, Virginia 23298 Massey Cancer Center, 
      Virginia Commonwealth University School of Medicine, Richmond, Virginia 23298 
      kdbaker@vcu.edu.
LA  - eng
GR  - R56 DK090984/DK/NIDDK NIH HHS/United States
GR  - 1R56DK090984-01A1/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20141230
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (HIF-1 protein, C elegans)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (blmp-1 protein, C elegans)
SB  - IM
MH  - Animals
MH  - Caenorhabditis elegans/*genetics/metabolism
MH  - Caenorhabditis elegans Proteins/genetics/*physiology
MH  - Cell Hypoxia
MH  - *Gene Expression Regulation
MH  - Mutation
MH  - Repressor Proteins
MH  - Signal Transduction
MH  - Transcription Factors/genetics/*physiology
MH  - *Transcription, Genetic
PMC - PMC4349068
OTO - NOTNLM
OT  - BLMP-1
OT  - C. elegans
OT  - HIF independent
OT  - hypoxia
EDAT- 2015/01/02 06:00
MHDA- 2015/12/15 06:00
PMCR- 2015/12/30
CRDT- 2015/01/02 06:00
PHST- 2015/01/02 06:00 [entrez]
PHST- 2015/01/02 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2015/12/30 00:00 [pmc-release]
AID - genetics.114.173989 [pii]
AID - 173989 [pii]
AID - 10.1534/genetics.114.173989 [doi]
PST - ppublish
SO  - Genetics. 2015 Mar;199(3):739-48. doi: 10.1534/genetics.114.173989. Epub 2014 Dec 
      30.