PMID- 25553799
OWN - NLM
STAT- MEDLINE
DCOM- 20160211
LR  - 20150312
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 6
IP  - 3
DP  - 2015 Mar
TI  - Enhanced action of apigenin and naringenin combination on estrogen receptor 
      activation in non-malignant colonocytes: implications on sorghum-derived 
      phytoestrogens.
PG  - 749-55
LID - 10.1039/c4fo00300d [doi]
AB  - Activation of estrogen receptor-beta (ERbeta) is an important mechanism for colon 
      cancer prevention. Specific sorghum varieties that contain flavones were shown to 
      activate ER in non-malignant colonocytes at low concentrations. This study aimed 
      to determine positive interactions among estrogenic flavonoids most relevant in 
      sorghum. Apigenin and naringenin were tested separately and in combination for 
      their ability to influence ER-mediated cell growth in non-malignant young adult 
      mouse colonocytes (YAMC). Sorghum extracts high in specific flavanones and 
      flavones were also tested. Apigenin reduced ER-mediated YAMC cell growth 
      comparable to physiological levels of estradiol (E(2), 1 nM) at 1 muM; naringenin 
      had similar effect at 10 muM. However, when combined, 0.1 muM apigenin plus 0.05 muM 
      naringenin produced similar effect as 1 nM E(2); these concentrations represented 
      1/10th and 1/200th, respectively, of the active concentrations of apigenin and 
      naringenin, demonstrating a strong enhanced action. A sorghum extract higher in 
      flavones (apigenin and luteolin) (4.8 mg g(-1)) was more effective (5 mug mL(-1)) 
      at activating ER in YAMC than a higher flavanone (naringenin and eriodictyol) 
      (28.1 mg g(-1)) sorghum extract (10 mug mL(-1)). Enhanced actions observed for 
      apigenin and naringenin were adequate to explain the level of effects produced by 
      the high flavone and flavanone sorghum extracts. Strong positive interactions 
      among sorghum flavonoids may enhance their ability to contribute to colon cancer 
      prevention beyond what can be modeled using target compounds in isolation.
FAU - Yang, Liyi
AU  - Yang L
AD  - Cereal Quality Laboratory, Soil & Crop Science Department, Texas A&M University, 
      College Station, Texas, USA. jawika@ag.tamu.edu.
FAU - Allred, Kimberly F
AU  - Allred KF
FAU - Dykes, Linda
AU  - Dykes L
FAU - Allred, Clinton D
AU  - Allred CD
FAU - Awika, Joseph M
AU  - Awika JM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 0 (Anticarcinogenic Agents)
RN  - 0 (Estrogen Receptor Antagonists)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Flavanones)
RN  - 0 (Phytoestrogens)
RN  - 0 (Pigments, Biological)
RN  - 0 (Plant Extracts)
RN  - 7V515PI7F6 (Apigenin)
RN  - HN5425SBF2 (naringenin)
RN  - KUX1ZNC9J2 (Luteolin)
RN  - Q520486B8Y (eriodictyol)
SB  - IM
MH  - Animals
MH  - Anticarcinogenic Agents/analysis/chemistry/isolation & purification/*pharmacology
MH  - Apigenin/agonists/chemistry/isolation & purification/*pharmacology
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Colon/*drug effects/metabolism
MH  - Colonic Neoplasms/metabolism/prevention & control
MH  - Drug Synergism
MH  - Estrogen Receptor Antagonists/pharmacology
MH  - Estrogen Receptor beta/agonists/antagonists & inhibitors/metabolism
MH  - Flavanones/agonists/analysis/chemistry/isolation & purification/*pharmacology
MH  - Functional Food/analysis
MH  - Intestinal Mucosa/*drug effects/metabolism
MH  - Luteolin/analysis/isolation & purification/pharmacology
MH  - Mice
MH  - Osmolar Concentration
MH  - Phytoestrogens/agonists/chemistry/isolation & purification/*pharmacology
MH  - Pigments, Biological/biosynthesis
MH  - Plant Extracts/chemistry/isolation & purification/pharmacology
MH  - Seeds/chemistry/metabolism
MH  - Sorghum/*chemistry/metabolism
EDAT- 2015/01/03 06:00
MHDA- 2016/02/13 06:00
CRDT- 2015/01/03 06:00
PHST- 2015/01/03 06:00 [entrez]
PHST- 2015/01/03 06:00 [pubmed]
PHST- 2016/02/13 06:00 [medline]
AID - 10.1039/c4fo00300d [doi]
PST - ppublish
SO  - Food Funct. 2015 Mar;6(3):749-55. doi: 10.1039/c4fo00300d.