PMID- 2555389
OWN - NLM
STAT- MEDLINE
DCOM- 19900109
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 27
IP  - 10
DP  - 1989 Oct
TI  - Establishment of titration system for human herpesvirus 6 and evaluation of 
      neutralizing antibody response to the virus.
PG  - 2204-7
AB  - The susceptibilities of seven T-cell lines to human herpesvirus 6 (HHV-6) 
      infection were examined. MT-4 cells were the most susceptible of these lines to 
      infection with this virus. Therefore, chemically adhered MT-4 cell monolayers 
      were used for infectious HHV-6 assay by indirect immunofluorescent-antibody (IFA) 
      staining. When cell monolayers were fixed 30 to 45 h postinfection, the foci 
      stained with IFA were easy to count and a linear relationship was observed 
      between the number of foci and the virus concentration. MT-4 cell monolayers were 
      also used for a focus reduction neutralizing-antibody test. In this test, sera 
      from patients in the convalescent stage of exanthem subitum all showed 
      significant neutralizing activity (1:80 to 1:320), whereas sera from patients in 
      the acute stage of disease showed no detectable neutralizing activity. The titers 
      of neutralizing antibody correlated well with the levels of anti-HHV-6 antibodies 
      detected by IFA.
FAU - Asada, H
AU  - Asada H
AD  - Department of Virology, Osaka University, Japan.
FAU - Yalcin, S
AU  - Yalcin S
FAU - Balachandra, K
AU  - Balachandra K
FAU - Higashi, K
AU  - Higashi K
FAU - Yamanishi, K
AU  - Yamanishi K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Antibodies, Viral/analysis/biosynthesis
MH  - Cell Line
MH  - Exanthema Subitum/immunology/microbiology
MH  - Herpesviridae/classification/immunology/*isolation & purification
MH  - Herpesviridae Infections/immunology
MH  - Humans
MH  - Infant
MH  - Neutralization Tests
MH  - T-Lymphocytes/immunology
PMC - PMC266994
EDAT- 1989/10/01 00:00
MHDA- 1989/10/01 00:01
PMCR- 1989/10/01
CRDT- 1989/10/01 00:00
PHST- 1989/10/01 00:00 [pubmed]
PHST- 1989/10/01 00:01 [medline]
PHST- 1989/10/01 00:00 [entrez]
PHST- 1989/10/01 00:00 [pmc-release]
AID - 10.1128/jcm.27.10.2204-2207.1989 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1989 Oct;27(10):2204-7. doi: 10.1128/jcm.27.10.2204-2207.1989.