PMID- 25555467
OWN - NLM
STAT- MEDLINE
DCOM- 20150929
LR  - 20181202
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 401
IP  - 1-2
DP  - 2015 Mar
TI  - The effect of extended passaging on the phenotype and osteogenic potential of 
      human umbilical cord mesenchymal stem cells.
PG  - 155-64
LID - 10.1007/s11010-014-2303-0 [doi]
AB  - Retaining biological characteristics in the extended passaging is crucial for 
      human umbilical cord mesenchymal stem cells (hUCMSCs) in tissue engineering. We 
      aimed to assess morphology, viability, MSC marker expression, and osteogenic 
      activity of hUCSMCs after extended passaging. Passages 4 (P4) and 16 (P16) 
      hUCMSCs displayed similar morphology and viability. The flow cytometry results 
      showed that CD73, CD90, and CD105 were highly expressed at P1-P16. CD166 
      expression decreased progressively from 90 % at P2 to 61.5 % at P5 (p < 0.05), 
      followed by stable expression through P16. Results from calcium deposition 
      alkaline phosphatase activity and RT-PCR assay showed that both P4 and P16 
      hUCMSCs differentiated down an osteogenic lineage, with no significant difference 
      in osteogenic capacity (p < 0.05). High-passage UMCSCs maintained stable 
      expression of MSC CD markers as well as stable osteogenic activity. hUCMSCs may 
      thus be suitable for tissue engineering and regenerative medicine applications.
FAU - Shi, Zhe
AU  - Shi Z
AD  - Department of Orthopaedic Surgery, The Third Affilliated Hospital, Southern 
      Medical University, Guangzhou, China.
FAU - Zhao, Liang
AU  - Zhao L
FAU - Qiu, Gengtao
AU  - Qiu G
FAU - He, Ruixuan
AU  - He R
FAU - Detamore, Michael S
AU  - Detamore MS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150103
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Biomarkers)
SB  - IM
MH  - Biomarkers/*metabolism
MH  - Cell Culture Techniques/*methods
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Female
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology
MH  - *Osteogenesis
MH  - Phenotype
MH  - Pregnancy
MH  - Tissue Engineering
MH  - Umbilical Cord/*cytology
EDAT- 2015/01/04 06:00
MHDA- 2015/09/30 06:00
CRDT- 2015/01/04 06:00
PHST- 2014/07/31 00:00 [received]
PHST- 2014/12/10 00:00 [accepted]
PHST- 2015/01/04 06:00 [entrez]
PHST- 2015/01/04 06:00 [pubmed]
PHST- 2015/09/30 06:00 [medline]
AID - 10.1007/s11010-014-2303-0 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2015 Mar;401(1-2):155-64. doi: 10.1007/s11010-014-2303-0. Epub 
      2015 Jan 3.