PMID- 25555495
OWN - NLM
STAT- MEDLINE
DCOM- 20160829
LR  - 20150419
IS  - 1768-3114 (Electronic)
IS  - 0369-8114 (Linking)
VI  - 63
IP  - 2
DP  - 2015 Apr
TI  - Family-based association study of HLA class II with type 1 diabetes in Moroccans.
PG  - 80-4
LID - S0369-8114(14)00173-4 [pii]
LID - 10.1016/j.patbio.2014.12.001 [doi]
AB  - BACKGROUND: The T1D is a multifactorial disease; with a strong genetic control. 
      The human leukocyte antigen (HLA) system plays a crucial role in the autoimmune 
      process leading to childhood diabetes. About 440,000 of the childhood population 
      of the world (1.8 billion children under 14 years of age), have type 1 diabetes, 
      and each year an additional 70,000 develop this disorder. The objective of this 
      study was to investigate the distribution of HLA class II in Moroccan families of 
      diabetic children to identify susceptibility alleles of the Moroccan population. 
      SUBJECTS AND METHODS: We included in this study, Moroccan families who have at 
      least one child with T1D. The age of onset of diabetes was less than 15 years. 
      HLA class II (DRB1* and DQB1*) was carried out by molecular biology techniques 
      (PCR-SSP and PCR-SSO). The FBAT test (family-based association test) was used to 
      highlight the association between T1D and the HLA-DRB1* and -DQB1* polymorphism. 
      RESULTS: The association of HLA class II (DRB1*, DQB1*) in type 1 diabetes was 
      analyzed in fifty-one Moroccan families, including 90 diabetics. The results 
      revealed that the most susceptible haplotypes are the DRB1*03:01-DQB1*02:01, 
      DRB1*04:05-DQB1*03:02 (Z=3.674, P=0.000239; Z=2.828, P=0.004678, respectively). 
      And the most protective haplotype is the DRB1*15-DQB1*06. CONCLUSION: This is the 
      first family-based association study searching for an association between HLA 
      class II and T1D in a Moroccan population. Despite the different ethnic groups 
      forming Morocco, Moroccan diabetics share the most susceptible and protective HLA 
      haplotypes with other Caucasians populations, specifically the European and 
      Mediterranean populations.
CI  - Copyright (c) 2014. Published by Elsevier SAS.
FAU - Drissi Bourhanbour, A
AU  - Drissi Bourhanbour A
AD  - UPR d'immunologie, faculte de medecine et de pharmacie, Rabat Institut, avenue 
      Mohamed Belarbi El Alaoui, BP 6203, 10000 Rabat, Morocco; Unite d'immunologie, 
      service de transfusion sanguine et d'hemovigilance, hopital d'enfants-Rabat, 
      Rabat Institut, CHU Ibn-Sina, rue Lamfadel Cherkaoui, BP 6527, Rabat, Morocco. 
      Electronic address: drissiasmaa@yahoo.fr.
FAU - Benseffaj, N
AU  - Benseffaj N
AD  - UPR d'immunologie, faculte de medecine et de pharmacie, Rabat Institut, avenue 
      Mohamed Belarbi El Alaoui, BP 6203, 10000 Rabat, Morocco; Unite d'immunologie, 
      service de transfusion sanguine et d'hemovigilance, hopital d'enfants-Rabat, 
      Rabat Institut, CHU Ibn-Sina, rue Lamfadel Cherkaoui, BP 6527, Rabat, Morocco.
FAU - Ouadghiri, S
AU  - Ouadghiri S
AD  - UPR d'immunologie, faculte de medecine et de pharmacie, Rabat Institut, avenue 
      Mohamed Belarbi El Alaoui, BP 6203, 10000 Rabat, Morocco; Unite d'immunologie, 
      service de transfusion sanguine et d'hemovigilance, hopital d'enfants-Rabat, 
      Rabat Institut, CHU Ibn-Sina, rue Lamfadel Cherkaoui, BP 6527, Rabat, Morocco.
FAU - Razine, R
AU  - Razine R
AD  - Laboratoire de biostatistique, recherche clinique et epidemiologie, faculte de 
      medecine et de pharmacie, Rabat Institut, avenue Mohamed Belarbi El Alaoui, BP 
      6203, 10000 Rabat, Morocco.
FAU - Touzani, A
AU  - Touzani A
AD  - Service de diabetologie pediatrique, hopital d'enfants-Rabat, Rabat Institut, CHU 
      Ibn-Sina, rue Lamfadel Cherkaoui, BP 6527, Rabat, Morocco.
FAU - Belafraj, A
AU  - Belafraj A
AD  - Service de diabetologie pediatrique, hopital d'enfants-Rabat, Rabat Institut, CHU 
      Ibn-Sina, rue Lamfadel Cherkaoui, BP 6527, Rabat, Morocco.
FAU - Essakalli, M
AU  - Essakalli M
AD  - UPR d'immunologie, faculte de medecine et de pharmacie, Rabat Institut, avenue 
      Mohamed Belarbi El Alaoui, BP 6203, 10000 Rabat, Morocco; Unite d'immunologie, 
      service de transfusion sanguine et d'hemovigilance, hopital d'enfants-Rabat, 
      Rabat Institut, CHU Ibn-Sina, rue Lamfadel Cherkaoui, BP 6527, Rabat, Morocco.
LA  - eng
PT  - Journal Article
DEP - 20141230
PL  - France
TA  - Pathol Biol (Paris)
JT  - Pathologie-biologie
JID - 0265365
RN  - 0 (HLA-D Antigens)
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DRB1 Chains)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Child, Preschool
MH  - Diabetes Mellitus, Type 1/epidemiology/*genetics
MH  - *Family
MH  - Female
MH  - Gene Frequency
MH  - *Genes, MHC Class II
MH  - *Genetic Predisposition to Disease
MH  - Genome-Wide Association Study/*methods
MH  - HLA-D Antigens/*genetics
MH  - HLA-DQ beta-Chains/genetics
MH  - HLA-DRB1 Chains/genetics
MH  - Humans
MH  - Male
MH  - Morocco/epidemiology
MH  - Polymorphism, Genetic
MH  - Young Adult
OTO - NOTNLM
OT  - Family-based association study
OT  - HLA class II
OT  - HLA classe II
OT  - Haplotypes
OT  - Type I diabetes
OT  - diabete type 1
OT  - Etude d'association familiale
EDAT- 2015/01/04 06:00
MHDA- 2016/08/30 06:00
CRDT- 2015/01/04 06:00
PHST- 2014/04/02 00:00 [received]
PHST- 2014/12/01 00:00 [accepted]
PHST- 2015/01/04 06:00 [entrez]
PHST- 2015/01/04 06:00 [pubmed]
PHST- 2016/08/30 06:00 [medline]
AID - S0369-8114(14)00173-4 [pii]
AID - 10.1016/j.patbio.2014.12.001 [doi]
PST - ppublish
SO  - Pathol Biol (Paris). 2015 Apr;63(2):80-4. doi: 10.1016/j.patbio.2014.12.001. Epub 
      2014 Dec 30.