PMID- 25555603
OWN - NLM
STAT- MEDLINE
DCOM- 20150924
LR  - 20181202
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 98
DP  - 2015 Feb
TI  - Expression profile of Toll-like receptor mRNA in pigs co-infected with porcine 
      reproductive and respiratory syndrome virus and porcine circovirus type 2.
PG  - 134-41
LID - S0034-5288(14)00344-0 [pii]
LID - 10.1016/j.rvsc.2014.12.003 [doi]
AB  - Field and experimental studies have shown that co-infection of pigs with porcine 
      reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 
      (PCV2) increases the severity of the disease. The present study investigates the 
      mRNA expression profile of Toll-like receptors (TLRs) in pigs co-infected with 
      PRRSV and PCV2. SPF pigs were infected with PRRSV, PCV2 or in a combination of 
      both. The mRNA expression levels of TLRs and related cytokines in peripheral 
      blood mononuclear cells (PBMCs) of pigs were determined by quantitative real-time 
      RT-PCR. The mRNA expression profiles of TLRs by PBMCs from pigs co-infected with 
      PRRSV and PCV2 displayed two distinct patterns: an increased expression profile 
      for TLRs2, 4 and 8, and a decreased expression profile for TLRs3, 7 and 9. An 
      up-regulated expression of IL-1beta and IL-10 mRNA and a down-regulated expression 
      of INF-alpha and TNF-alpha mRNA in PBMCs of co-infected pigs were also observed.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Tu, Pang-Yan
AU  - Tu PY
AD  - Department of Veterinary Medicine, National Pingtung University of Science and 
      Technology, Neipu, Pingtung 912, Taiwan.
FAU - Tsai, Pei-Chun
AU  - Tsai PC
AD  - Department of Veterinary Medicine, National Pingtung University of Science and 
      Technology, Neipu, Pingtung 912, Taiwan.
FAU - Lin, Yi-Hsin
AU  - Lin YH
AD  - Department of Veterinary Medicine, National Pingtung University of Science and 
      Technology, Neipu, Pingtung 912, Taiwan.
FAU - Liu, Po-Cheng
AU  - Liu PC
AD  - Department of Veterinary Medicine, National Pingtung University of Science and 
      Technology, Neipu, Pingtung 912, Taiwan.
FAU - Chang, Hsiu-luan
AU  - Chang HL
AD  - Department of Animal Science, National Pingtung University of Science and 
      Technology, Neipu, Pingtung 912, Taiwan. Electronic address: 
      hlachang@mail.npust.edu.tw.
FAU - Kuo, Tsun-Yung
AU  - Kuo TY
AD  - Department of Biotechnology and Animal Science, National Ilan University, Ilan 
      26041, Taiwan.
FAU - Chung, Wen-Bin
AU  - Chung WB
AD  - Department of Veterinary Medicine, National Pingtung University of Science and 
      Technology, Neipu, Pingtung 912, Taiwan. Electronic address: 
      wbchung@mail.npust.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141210
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Animals
MH  - Circoviridae Infections/immunology/*veterinary/virology
MH  - Circovirus/immunology
MH  - Coinfection/immunology/*veterinary/virology
MH  - Cytokines/genetics/immunology
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*immunology
MH  - Leukocytes, Mononuclear/*immunology/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/immunology
MH  - RNA, Messenger/genetics/metabolism
MH  - Random Allocation
MH  - Swine
MH  - Swine Diseases/*immunology/virology
MH  - *Toll-Like Receptors/genetics/immunology
OTO - NOTNLM
OT  - Co-infection
OT  - Pigs
OT  - Porcine circovirus type 2
OT  - Porcine reproductive and respiratory syndrome virus
OT  - Toll-like receptors
EDAT- 2015/01/04 06:00
MHDA- 2015/09/25 06:00
CRDT- 2015/01/04 06:00
PHST- 2014/07/31 00:00 [received]
PHST- 2014/11/23 00:00 [revised]
PHST- 2014/12/03 00:00 [accepted]
PHST- 2015/01/04 06:00 [entrez]
PHST- 2015/01/04 06:00 [pubmed]
PHST- 2015/09/25 06:00 [medline]
AID - S0034-5288(14)00344-0 [pii]
AID - 10.1016/j.rvsc.2014.12.003 [doi]
PST - ppublish
SO  - Res Vet Sci. 2015 Feb;98:134-41. doi: 10.1016/j.rvsc.2014.12.003. Epub 2014 Dec 
      10.