PMID- 25556717
OWN - NLM
STAT- MEDLINE
DCOM- 20151217
LR  - 20220408
IS  - 1398-9995 (Electronic)
IS  - 0105-4538 (Linking)
VI  - 70
IP  - 4
DP  - 2015 Apr
TI  - IgE+ B cells are scarce, but allergen-specific B cells with a memory phenotype 
      circulate in patients with allergic rhinitis.
PG  - 420-8
LID - 10.1111/all.12563 [doi]
AB  - BACKGROUND: Despite the critical role of immunoglobulin E (IgE) in allergy, 
      circulating IgE+ B cells are scarce. Here, we describe in patients with allergic 
      rhinitis B cells with a memory phenotype responding to a prototypic aeroallergen. 
      METHODS: Fifteen allergic rhinitis patients with grass pollen allergy and 13 
      control subjects were examined. Blood mononuclear cells stained with 
      carboxyfluorescein diacetate succinimidyl ester (CFSE) were cultured with Bahia 
      grass pollen. Proliferation and phenotype were assessed by multicolour flow 
      cytometry. RESULTS: In blood of allergic rhinitis patients with high serum IgE to 
      grass pollen, most IgE(hi) cells were CD123+ HLA-DR(-) basophils, with IgE for 
      the major pollen allergen (Pas n 1). Both B and T cells from pollen-allergic 
      donors showed higher proliferation to grass pollen than nonallergic donors (P = 
      0.002, and 0.010, respectively), whereas responses to vaccine antigens and 
      mitogen did not differ between groups. Allergen-driven B cells that divided 
      rapidly (CD19(mid) CD3(-) CFSE(lo) ) showed higher CD27 (P = 0.008) and lower 
      CD19 (P = 0.004) and CD20 (P = 0.004) expression than B cells that were slow to 
      respond to allergen (CD19(hi) CD3(-) CFSE(mid) ). Moreover, rapidly dividing 
      allergen-driven B cells (CD19(mid) CFSE(lo) CD27(hi) ) showed higher expression 
      of the plasmablast marker CD38 compared with B cells (CD19(hi) CFSE(mid) CD27(lo) 
      ) that were slow to divide. CONCLUSION: Patients with pollen allergy but not 
      control donors have a population of circulating allergen-specific B cells with 
      the phenotype and functional properties of adaptive memory B-cell responses. 
      These cells could provide precursors for allergen-specific IgE production upon 
      allergen re-exposure.
CI  - (c) 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Wong, K J
AU  - Wong KJ
AD  - Lung and Allergy Research Centre, School of Medicine, The University of 
      Queensland, Woolloongabba, Qld, Australia.
FAU - Timbrell, V
AU  - Timbrell V
FAU - Xi, Y
AU  - Xi Y
FAU - Upham, J W
AU  - Upham JW
FAU - Collins, A M
AU  - Collins AM
FAU - Davies, J M
AU  - Davies JM
LA  - eng
PT  - Journal Article
DEP - 20150202
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Allergens)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Allergens/*immunology/metabolism
MH  - B-Lymphocyte Subsets/*immunology/*metabolism
MH  - Case-Control Studies
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/blood/*immunology/metabolism
MH  - Immunoglobulin G/blood/immunology
MH  - *Immunologic Memory
MH  - Lymphocyte Activation
MH  - Male
MH  - Middle Aged
MH  - Phenotype
MH  - Poaceae/adverse effects
MH  - Pollen/immunology
MH  - Protein Binding/immunology
MH  - Rhinitis, Allergic/diagnosis/*immunology/*metabolism
MH  - Rhinitis, Allergic, Seasonal/diagnosis/immunology/metabolism
MH  - Young Adult
OTO - NOTNLM
OT  - B cells
OT  - allergic rhinitis
OT  - grass pollen
OT  - immunoglobulin E
OT  - immunoglobulin E B cells
EDAT- 2015/01/06 06:00
MHDA- 2015/12/19 06:00
CRDT- 2015/01/06 06:00
PHST- 2014/12/19 00:00 [accepted]
PHST- 2015/01/06 06:00 [entrez]
PHST- 2015/01/06 06:00 [pubmed]
PHST- 2015/12/19 06:00 [medline]
AID - 10.1111/all.12563 [doi]
PST - ppublish
SO  - Allergy. 2015 Apr;70(4):420-8. doi: 10.1111/all.12563. Epub 2015 Feb 2.