PMID- 25557089
OWN - NLM
STAT- MEDLINE
DCOM- 20160104
LR  - 20220318
IS  - 1469-8137 (Electronic)
IS  - 0028-646X (Linking)
VI  - 206
IP  - 2
DP  - 2015 Apr
TI  - Characterization of two CENH3 genes and their roles in wheat evolution.
PG  - 839-51
LID - 10.1111/nph.13235 [doi]
AB  - Wheat evolution is complex as a result of successive rounds of 
      allopolyploidization and continuous selection during domestication. Diploid and 
      tetraploid wheat species (Triticum spp.) were used as model systems in which to 
      study the role of centromere-specific histone H3 variant (CENH3) in wheat 
      evolution. We characterized two types of CENH3 genes, named alphaCENH3 and betaCENH3, 
      each of which has three slightly different copies derived from the AA, BB and DD 
      genomes. Specific antibodies were raised against the two CENH3 proteins and were 
      co-localized to centromeres with subtle differences. In most tetraploid wheat 
      species, CENH3 genes are more highly expressed from the AA genome. In wild 
      tetraploids, betaCENH3 has a much lower expression level than alphaCENH3, while in 
      cultivated tetraploids betaCENH3 transcripts are enhanced to near alphaCENH3 levels. 
      Comparison of the CENH3 proteins in wild and cultivated tetraploids revealed that 
      the histone folding domain (HFD) of only betaCENH3 is under positive selection, 
      especially in the region responsible for targeting of CENH3 to the centromere. 
      Taken together, positive selection of betaCENH3 and its increased expression in 
      tetraploid cultivars are indicative of adaptive evolution. Furthermore, the 
      differences in localization between alphaCENH3 and betaCENH3 observed using fiber 
      fluorescence in situ hybridization (FISH) and immunodetection and in 
      developmental phenotypes resulting from virus-reduced gene silencing imply their 
      functional diversification during wheat evolution.
CI  - (c) 2014 The Authors. New Phytologist (c) 2014 New Phytologist Trust.
FAU - Yuan, Jing
AU  - Yuan J
AD  - State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of 
      Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, 
      China.
FAU - Guo, Xiang
AU  - Guo X
FAU - Hu, Jing
AU  - Hu J
FAU - Lv, Zhenling
AU  - Lv Z
FAU - Han, Fangpu
AU  - Han F
LA  - eng
SI  - GENBANK/JF969286
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141231
PL  - England
TA  - New Phytol
JT  - The New phytologist
JID - 9882884
RN  - 0 (Histones)
RN  - 0 (Plant Proteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Adaptation, Biological
MH  - Amino Acid Sequence
MH  - Biological Evolution
MH  - Centromere/genetics
MH  - Chromosomes, Plant/genetics
MH  - Genome, Plant/*genetics
MH  - Histones/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Plant Leaves/genetics
MH  - Plant Proteins/genetics
MH  - Polyploidy
MH  - Recombinant Proteins
MH  - Selection, Genetic
MH  - Sequence Alignment
MH  - Triticum/*genetics
OTO - NOTNLM
OT  - CENH3
OT  - centromere
OT  - gene expression
OT  - polyploid
OT  - wheat (Triticum spp.)
EDAT- 2015/01/06 06:00
MHDA- 2016/01/05 06:00
CRDT- 2015/01/06 06:00
PHST- 2014/09/27 00:00 [received]
PHST- 2014/11/13 00:00 [accepted]
PHST- 2015/01/06 06:00 [entrez]
PHST- 2015/01/06 06:00 [pubmed]
PHST- 2016/01/05 06:00 [medline]
AID - 10.1111/nph.13235 [doi]
PST - ppublish
SO  - New Phytol. 2015 Apr;206(2):839-51. doi: 10.1111/nph.13235. Epub 2014 Dec 31.