PMID- 25557667
OWN - NLM
STAT- MEDLINE
DCOM- 20150921
LR  - 20240109
IS  - 1879-0445 (Electronic)
IS  - 0960-9822 (Linking)
VI  - 25
IP  - 2
DP  - 2015 Jan 19
TI  - ELF3-PIF4 interaction regulates plant growth independently of the Evening 
      Complex.
PG  - 187-193
LID - S0960-9822(14)01418-3 [pii]
LID - 10.1016/j.cub.2014.10.070 [doi]
AB  - The circadian clock plays a pivotal role in the control of Arabidopsis hypocotyl 
      elongation by regulating rhythmic expression of the bHLH factors PHYTOCHROME 
      INTERACTING FACTOR 4 and 5 (PIF4 and 5). Coincidence of increased PIF4/PIF5 
      transcript levels with the dark period allows nuclear accumulation of these 
      factors, and in short days it phases maximal hypocotyl growth at dawn. During 
      early night, PIF4 and PIF5 transcription is repressed by the Evening Complex (EC) 
      proteins EARLY FLOWERING3 (ELF3), EARLY FLOWERING4 (ELF4), and LUX ARRHYTHMO 
      (LUX). While ELF3 has an essential role in EC complex assembly, several lines of 
      evidence indicate that this protein controls plant growth via other mechanisms 
      that are presently unknown. Here, we show that the ELF3 and PIF4 proteins 
      interact in an EC-independent manner, and that this interaction prevents PIF4 
      from activating its transcriptional targets. We also show that PIF4 
      overexpression leads to ELF3 protein destabilization, and that this effect is 
      mediated indirectly by negative feedback regulation of photoactive PHYTOCHROME B 
      (phyB). Physical interaction of the phyB photoreceptor with ELF3 has been 
      reported, but its functional relevance remains poorly understood. Our findings 
      establish that phyB is needed for ELF3 accumulation in the light, most likely by 
      competing for CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1)-mediated ubiquitination and 
      the proteasomal degradation of ELF3. Our results explain the short hypocotyl 
      phenotype of ELF3 overexpressors, despite their normal clock function, and 
      provide a molecular framework for understanding how warm temperatures promote 
      hypocotyl elongation and affect the endogenous clock.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Nieto, Cristina
AU  - Nieto C
AD  - Departamento Genetica Molecular de Plantas, Centro Nacional de Biotecnologia 
      (CSIC), Campus de Cantoblanco, Darwin 3, 28049 Madrid, Spain.
FAU - Lopez-Salmeron, Vadir
AU  - Lopez-Salmeron V
AD  - Departamento Genetica Molecular de Plantas, Centro Nacional de Biotecnologia 
      (CSIC), Campus de Cantoblanco, Darwin 3, 28049 Madrid, Spain.
FAU - Daviere, Jean-Michel
AU  - Daviere JM
AD  - Departamento Genetica Molecular de Plantas, Centro Nacional de Biotecnologia 
      (CSIC), Campus de Cantoblanco, Darwin 3, 28049 Madrid, Spain.
FAU - Prat, Salome
AU  - Prat S
AD  - Departamento Genetica Molecular de Plantas, Centro Nacional de Biotecnologia 
      (CSIC), Campus de Cantoblanco, Darwin 3, 28049 Madrid, Spain. Electronic address: 
      sprat@cnb.csic.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141231
PL  - England
TA  - Curr Biol
JT  - Current biology : CB
JID - 9107782
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (ELF3 protein, Arabidopsis)
RN  - 0 (PIF4 protein, Arabidopsis)
RN  - 0 (Transcription Factors)
RN  - 136250-22-1 (Phytochrome B)
SB  - IM
MH  - Arabidopsis/*genetics/*growth & development
MH  - Arabidopsis Proteins/*genetics/metabolism
MH  - Basic Helix-Loop-Helix Transcription Factors/*genetics/metabolism
MH  - Circadian Clocks
MH  - Gene Expression Regulation, Plant
MH  - Light
MH  - Phytochrome B/metabolism
MH  - Polymerase Chain Reaction
MH  - Transcription Factors/*genetics/metabolism
EDAT- 2015/01/06 06:00
MHDA- 2015/09/22 06:00
CRDT- 2015/01/06 06:00
PHST- 2014/08/25 00:00 [received]
PHST- 2014/10/22 00:00 [revised]
PHST- 2014/10/22 00:00 [accepted]
PHST- 2015/01/06 06:00 [entrez]
PHST- 2015/01/06 06:00 [pubmed]
PHST- 2015/09/22 06:00 [medline]
AID - S0960-9822(14)01418-3 [pii]
AID - 10.1016/j.cub.2014.10.070 [doi]
PST - ppublish
SO  - Curr Biol. 2015 Jan 19;25(2):187-193. doi: 10.1016/j.cub.2014.10.070. Epub 2014 
      Dec 31.