PMID- 25558272
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150105
LR  - 20200928
IS  - 0974-5963 (Print)
IS  - 1742-6413 (Electronic)
IS  - 1742-6413 (Linking)
VI  - 11
DP  - 2014
TI  - Diagnostic accuracy of bronchial brush cytology and the added value of 
      immunohistochemistry and fluorescence in situ hybridization of pulmonary 
      neuroendocrine tumors.
PG  - 32
LID - 10.4103/1742-6413.146120 [doi]
LID - 32
AB  - BACKGROUND: Bronchial brush (BB) cytology carries low sensitivity for detecting 
      neuroendocrine carcinomas (NECs), including typical carcinoid (TC) tumors of the 
      lung. We aimed to investigate the detection of neuroendocrine tumors including TC 
      through BB routine cytology cell block (CB), immunohistochemistry (IHC), and 
      fluorescence in situ hybridization (FISH). MATERIALS AND METHODS: A SNOMED search 
      showed 187 lung biopsy or resection specimens from 2008 through 2011 containing 
      neuroendocrine or carcinoid in the diagnosis. Residual BB specimens retained in 
      PreservCyt were used to prepare a ThinPrep slide for FISH analysis. CBs were 
      stained with H and E and IHC for chromogranin and synaptophysin. RESULTS: Of the 
      187 cases, 16 had residual BB material available within 1 year of diagnosis and 
      were used in CB preparation for IHC and FISH slides. Cytologic evaluation 
      determined 1 case positive for malignancy (small cell lung carcinoma [SCLC]), 1 
      suspicious for adenocarcinoma, and 14 negative for malignancy. On the basis of 
      histologic diagnosis, FISH was performed. SCLC showed polysomy (86% abnormal 
      cells); 2 TC tumors showed a gain of 7p12 (15% abnormal cells) and a gain of 5q15 
      (72% abnormal cells), respectively. Two cases had CBs with positive 
      immunoreactivity for chromogranin and synaptophysin. The sensitivity for 
      detection of NEC was 18.8%, 15.4%, and 25% for cytologic evaluation, CB, and 
      FISH, respectively. CONCLUSION: Neuroendocrine tumors, including TC are difficult 
      to detect with BB cytologic evaluation, most likely because tumor cells lack in 
      the specimen. Assessment of further studies is needed to explore the role of 
      cytology and ancillary methods for detection of these tumors.
FAU - Reynolds, Jordan P
AU  - Reynolds JP
AD  - Address: Pathology and Laboratory Medicine Institute, Cleveland Clinic, 
      Cleveland, OH, United States.
FAU - Voss, Jesse S
AU  - Voss JS
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, 
      United States.
FAU - Brankley, Shannon M
AU  - Brankley SM
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, 
      United States.
FAU - Caudill, Jill M
AU  - Caudill JM
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, 
      United States.
FAU - Henry, Michael R
AU  - Henry MR
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, 
      United States.
FAU - Clayton, Amy C
AU  - Clayton AC
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, 
      United States.
FAU - Halling, Kevin C
AU  - Halling KC
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, 
      United States.
FAU - Nassar, Aziza
AU  - Nassar A
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Jacksonville, FL, 
      United States.
LA  - eng
PT  - Journal Article
DEP - 20141203
PL  - United States
TA  - Cytojournal
JT  - CytoJournal
JID - 101231642
PMC - PMC4278144
OTO - NOTNLM
OT  - Ancillary studies
OT  - carcinoid tumor
OT  - cytology
OT  - endobronchial sampling
OT  - pulmonary
EDAT- 2015/01/06 06:00
MHDA- 2015/01/06 06:01
PMCR- 2014/12/03
CRDT- 2015/01/06 06:00
PHST- 2014/07/11 00:00 [received]
PHST- 2014/10/09 00:00 [accepted]
PHST- 2015/01/06 06:00 [entrez]
PHST- 2015/01/06 06:00 [pubmed]
PHST- 2015/01/06 06:01 [medline]
PHST- 2014/12/03 00:00 [pmc-release]
AID - CJ-11-32 [pii]
AID - 10.4103/1742-6413.146120 [doi]
PST - epublish
SO  - Cytojournal. 2014 Dec 3;11:32. doi: 10.4103/1742-6413.146120. eCollection 2014.