PMID- 25559146
OWN - NLM
STAT- MEDLINE
DCOM- 20160321
LR  - 20181202
IS  - 1477-2566 (Electronic)
IS  - 1465-3249 (Linking)
VI  - 17
IP  - 4
DP  - 2015 Apr
TI  - Preoperative exercise facilitates abundant bone marrow collection in patients 
      with type 2 diabetes for mononuclear cell therapy.
PG  - 454-7
LID - S1465-3249(14)00858-5 [pii]
LID - 10.1016/j.jcyt.2014.11.007 [doi]
AB  - BACKGROUND AIMS: Traditional bone marrow (BM) collection is inadequate for 
      separation of abundant mononuclear cells (MNCs). We aimed to investigate the 
      effects of preoperative exercise on BM collection in patients with type 2 
      diabetes mellitus (T2DM). METHODS: Sixty patients with T2DM were randomly 
      assigned to either a control group or an exercise group (n = 30 each). The 
      patients in the exercise group exercised before the collection. All patients 
      underwent routine surgical care. The collected BM volume, operation duration, 
      collecting speed, puncture times and pain scores were recorded. BM samples were 
      tested before and after MNCs separation for CD34+ flow cytometry and whole blood 
      cell count. RESULTS: The collected BM volumes were significantly larger and 
      collection speed was faster in the exercise group (379.77 +/- 4.93 mL and 1.40 +/- 
      0.14 mL/s) than those in the control group (356.67 +/- 15.36 mL and 0.89 +/- 0.16 
      mL/s, P = 0.00 for both). Puncture times were significantly less and pain scores 
      were lower in the exercise group (2.07 +/- 0.25 and 2.67 +/- 1.56) than those in the 
      control group (2.50 +/- 0.63 and 3.43 +/- 1.76, P = 0.00 and 0.02, respectively). 
      CD34+ cells and whole blood cell count variables were comparable in the 2 groups. 
      CONCLUSIONS: Preoperative exercise facilitates BM collection by increasing 
      collected volume, improving collecting speed, relieving patients' pains and 
      ensuring MNC quality.
CI  - Copyright (c) 2015 International Society for Cellular Therapy. Published by 
      Elsevier Inc. All rights reserved.
FAU - Wu, Zhixian
AU  - Wu Z
AD  - Organ Transplant Institute, Fuzhou General Hospital (Dongfang Hospital), Xiamen 
      University, Fuzhou, People's Republic of China.
FAU - Luo, Fang
AU  - Luo F
AD  - Organ Transplant Institute, Fuzhou General Hospital (Dongfang Hospital), Xiamen 
      University, Fuzhou, People's Republic of China.
FAU - Wu, Zhifen
AU  - Wu Z
AD  - Obstetrics and Gynecology Department, Quanzhou First Hospital, Quanzhou, People's 
      Republic of China.
FAU - Tao, Xiaoqin
AU  - Tao X
AD  - Organ Transplant Institute, Fuzhou General Hospital (Dongfang Hospital), Xiamen 
      University, Fuzhou, People's Republic of China.
FAU - Zou, Xuelian
AU  - Zou X
AD  - Organ Transplant Institute, Fuzhou General Hospital (Dongfang Hospital), Xiamen 
      University, Fuzhou, People's Republic of China.
FAU - Tan, Jianming
AU  - Tan J
AD  - Organ Transplant Institute, Fuzhou General Hospital (Dongfang Hospital), Xiamen 
      University, Fuzhou, People's Republic of China. Electronic address: 
      doctortjm@yahoo.com.
LA  - eng
SI  - ClinicalTrials.gov/NCT00767260
PT  - Journal Article
PT  - Randomized Controlled Trial
DEP - 20150102
PL  - England
TA  - Cytotherapy
JT  - Cytotherapy
JID - 100895309
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bone Marrow Cells/*cytology
MH  - Cell Separation/*methods
MH  - *Cell- and Tissue-Based Therapy
MH  - Diabetes Mellitus, Type 2/*physiopathology/therapy
MH  - Exercise/*physiology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*cytology/transplantation
MH  - Preoperative Care
OTO - NOTNLM
OT  - bone marrow
OT  - exercise
OT  - mononuclear cells
OT  - type 2 diabetes
EDAT- 2015/01/07 06:00
MHDA- 2016/03/22 06:00
CRDT- 2015/01/07 06:00
PHST- 2014/09/09 00:00 [received]
PHST- 2014/11/16 00:00 [revised]
PHST- 2014/11/18 00:00 [accepted]
PHST- 2015/01/07 06:00 [entrez]
PHST- 2015/01/07 06:00 [pubmed]
PHST- 2016/03/22 06:00 [medline]
AID - S1465-3249(14)00858-5 [pii]
AID - 10.1016/j.jcyt.2014.11.007 [doi]
PST - ppublish
SO  - Cytotherapy. 2015 Apr;17(4):454-7. doi: 10.1016/j.jcyt.2014.11.007. Epub 2015 Jan 
      2.